{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:25:36Z","timestamp":1783013136958,"version":"3.54.6"},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T00:00:00Z","timestamp":1432252800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present study, antigens from parthenogenetic females and eggs of<jats:italic>Strongyloides venezuelensis<\/jats:italic>, or anti-parthenogenetic-female and anti-egg antigens were used to detect specific IgG and immune complex responses, respectively. Serum samples from experimentally infected immunocompetent and immunosuppressed rats were analysed on days 5, 8, 13 and 21 post-infection (dpi). An enzyme-linked immunosorbent assay (ELISA) was performed using alkaline parasite extract for specific IgG detection, and anti-parthenogenetic-female or anti-egg antigens for immune complex detection. The data were analysed using analysis of variance (ANOVA), followed by a Bonferroni test. When parthenogenetic female or egg extracts were used as antigens, specific IgGs were not detected in either immunocompetent or immunosuppressed rats. When anti-parthenogenetic-female or anti-<jats:italic>S. venezuelensis<\/jats:italic>-eggs were used, immune complexes were detected for the duration of the infection in immunosuppressed animals and were only detected between 5 and 13 dpi in immunocompetent animals. The duration of infection was not significantly different between the immunocompetent and immunosuppressed groups when anti-parthenogenetic-female or anti-<jats:italic>S. venezuelensis-<\/jats:italic>eggs were used. Parthenogenetic female extracts yielded significant differences between antibody and immune complex responses in immunocompetent rats from 5 to 13 dpi, but only on day 5 dpi in immunosuppressed rats. Exposure to<jats:italic>S. venezuelensis<\/jats:italic>egg extract yielded significant differences in both antibody and immune complex detection between immunocompetent and immunosuppressed rats for the duration of the infection. In conclusion, ELISA using alternative antigens may be a successful strategy for identifying immune complexes in serum samples and diagnosing active strongyloidiasis, particularly under conditions of immunosuppression.<\/jats:p>","DOI":"10.1017\/s0022149x15000346","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T05:06:18Z","timestamp":1432271178000},"page":"342-346","source":"Crossref","is-referenced-by-count":11,"title":["Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode<i>Strongyloides venezuelensis<\/i>for the diagnosis of immunosuppression in infected rats"],"prefix":"10.1017","volume":"90","author":[{"given":"A.L.R.","family":"Gon\u00e7alves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.C.L.","family":"de Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.F.G.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.T.","family":"Ueta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Costa-Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2015,5,22]]},"reference":[{"key":"S0022149X15000346_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.01.007"},{"key":"S0022149X15000346_ref13","doi-asserted-by":"crossref","first-page":"5","DOI":"10.53393\/rial.1954.14.33246","article-title":"Nova t\u00e9cnica para isolar larvas de nemat\u00f3ides das fezes: Modifica\u00e7\u00e3o do m\u00e9todo de Baermann","volume":"14","author":"Rugai","year":"1954","journal-title":"Revista do Instituto Adolfo Lutz"},{"key":"S0022149X15000346_ref16","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00118"},{"key":"S0022149X15000346_ref9","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762003000600024"},{"key":"S0022149X15000346_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60036-6"},{"key":"S0022149X15000346_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2013.09.004"},{"key":"S0022149X15000346_ref8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"S0022149X15000346_ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050355"},{"key":"S0022149X15000346_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.07.010"},{"key":"S0022149X15000346_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(90)90173-K"},{"key":"S0022149X15000346_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.01.016"},{"key":"S0022149X15000346_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.09.011"},{"key":"S0022149X15000346_ref6","first-page":"50","article-title":"New technique for counting nematode eggs in sheep faeces","volume":"12","author":"Gordon","year":"1939","journal-title":"Journal of the Council for Scientific and Industrial Research"},{"key":"S0022149X15000346_ref15","first-page":"215","volume-title":"Immunofluorescence and related techniques","author":"Wilson","year":"1978"},{"key":"S0022149X15000346_ref10","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652013000200015"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X15000346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T04:51:25Z","timestamp":1717908685000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X15000346\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,22]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["S0022149X15000346"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x15000346","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,22]]}}}