{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:58:59Z","timestamp":1777417139288,"version":"3.51.4"},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Strongyloides venezuelensis<\/jats:italic>is an intestinal nematode of rats, frequently used as a model for studying human and animal strongyloidiasis. In the present study, we evaluated parasitological, serological and molecular methods for the diagnosis of experimental<jats:italic>S. venezuelensis<\/jats:italic>in rats,<jats:italic>Rattus norvegicus<\/jats:italic>. Blood and faecal samples were collected and analysed up to 60 days post infection (pi) with adult worm recovery occurring from 5 to 45 days pi. Using an enzyme-linked immunosorbent assay (ELISA), serum levels of IgG antibodies increased up to 28 days pi, thereafter decreasing by day 60 pi. Polymerase chain reaction (PCR) assays detected<jats:italic>S. venezuelensis<\/jats:italic>DNA in faecal samples of rats from 5 to 21 days pi. The present study therefore represents the first step towards improving the diagnosis of experimental strongyloidiasis.<\/jats:p>","DOI":"10.1017\/s0022149x15000528","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T03:14:15Z","timestamp":1436843655000},"page":"422-427","source":"Crossref","is-referenced-by-count":9,"title":["Diagnosis of the strongyloid nematode<i>Strongyloides venezuelensis<\/i>in experimentally infected rats"],"prefix":"10.1017","volume":"90","author":[{"given":"P.D.","family":"Marques","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.M.","family":"Malta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.M.C.L.","family":"Meisel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Corral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.R.","family":"Pinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Costa-Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.P.","family":"Chieffi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.C.B.","family":"Gryschek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.M.","family":"Paula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"S0022149X15000528_ref12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"S0022149X15000528_ref22","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013002035"},{"key":"S0022149X15000528_ref13","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762003000600024"},{"key":"S0022149X15000528_ref3","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651997000600001"},{"key":"S0022149X15000528_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2009.02.013"},{"key":"S0022149X15000528_ref23","doi-asserted-by":"crossref","first-page":"203","DOI":"10.7476\/9788570785237.0009","volume-title":"Pesquisa na \u00e1rea biom\u00e9dica: do planejamento \u00e0 publica\u00e7\u00e3o","author":"Sopelete","year":"2005"},{"key":"S0022149X15000528_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2008.12.001"},{"key":"S0022149X15000528_ref14","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762010000100008"},{"key":"S0022149X15000528_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.01.012"},{"key":"S0022149X15000528_ref8","first-page":"50","article-title":"New technique for counting nematode eggs in sheep faeces","volume":"12","author":"Gordon","year":"1939","journal-title":"Journal of Scientific and Industrial Research"},{"key":"S0022149X15000528_ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-009-0745-1"},{"key":"S0022149X15000528_ref16","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3283551dbd"},{"key":"S0022149X15000528_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2011.05.006"},{"key":"S0022149X15000528_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01160.x"},{"key":"S0022149X15000528_ref17","first-page":"1","article-title":"Infec\u00e7\u00e3o experimental de camundongos (Mus musculus) e ratos (Rattus norvegicus) com Strongyloides venezuelensis","volume":"10","author":"Nakai","year":"2001","journal-title":"Revista Brasileira de Parasitologia Veterin\u00e1ria"},{"key":"S0022149X15000528_ref11","first-page":"163","article-title":"The anatomy and life history of Ancylostoma duodenale Dub. 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