{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:45:25Z","timestamp":1792691125812,"version":"4.5.0"},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T00:00:00Z","timestamp":1452729600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study we present the first approach to exploration of the genetic diversity of Cuban <jats:italic>Fasciola hepatica<\/jats:italic> populations using microsatellite markers, coupled with observed prevalence in slaughterhouses. Nine populations of flukes recovered from cows and buffalos were studied in the central-western region of Cuba. The observed infection rates of definitive hosts (bovines) were 70\u2013100% in most cases. An important amount of polymorphism was found in the four loci explored. However, no apparent genetic differences were found between populations from different provinces or bovine species. The absence of deviations from Hardy\u2013Weinberg equilibrium suggests a high rate of cross-fertilization between <jats:italic>F. hepatica<\/jats:italic> individuals. This result was confirmed when all multilocus genotypes were tested for clonal reproduction and only four individuals differed statistically (<jats:italic>P<\/jats:italic><jats:sub>sex<\/jats:sub>&lt;\u00a00.05). High values of expected heterozygosity coupled with highly probable mixing among strains make the metapopulation genetically diversified but similar in terms of certain alleles (low <jats:italic>F<\/jats:italic><jats:sub>ST<\/jats:sub> values). These results suggest a close relationship between parasite diversity and cattle management in Cuba. Our findings should be taken into consideration by veterinary authorities to help mitigate fasciolosis transmission.<\/jats:p>","DOI":"10.1017\/s0022149x15001029","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T10:18:56Z","timestamp":1452766736000},"page":"719-725","source":"Crossref","is-referenced-by-count":15,"title":["Genetic and infective diversity of the liver fluke <i>Fasciola hepatica<\/i> (Trematoda: Digenea) from Cuba"],"prefix":"10.1017","volume":"90","author":[{"given":"A.A.","family":"V\u00e1zquez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lounnas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Alba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Milesi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Hurtrez-Bouss\u00e8s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2016,1,14]]},"reference":[{"key":"S0022149X15001029_ref32","first-page":"248","article-title":"Distribuci\u00f3n y preferencia de h\u00e1bitats de moluscos hospederos intermediarios de Fasciola hepatica en Cuba","volume":"61","author":"V\u00e1zquez","year":"2009","journal-title":"Revista Cubana de Medicina Tropical"},{"key":"S0022149X15001029_ref29","first-page":"107","article-title":"Fascioliasis due to Fasciola hepatica and Fasciola gigantica infection: an update on this \u2018neglected\u2019 neglected tropical disease","volume":"42","author":"Tolan","year":"2011","journal-title":"LabMedicine"},{"key":"S0022149X15001029_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-5347(03)00223-4"},{"key":"S0022149X15001029_ref10","doi-asserted-by":"publisher","DOI":"10.4081\/gh.2006.281"},{"key":"S0022149X15001029_ref30","first-page":"127","article-title":"Genetic characterization of Cuban Creole cattle using molecular tools","volume":"29","author":"Uffo","year":"2012","journal-title":"Biotecnolog\u00eda Aplicada"},{"key":"S0022149X15001029_ref24","first-page":"45","article-title":"B\u00fafalos de Agua en Cuba: Or\u00edgen y evoluci\u00f3n","volume":"3","author":"Mitat","year":"2009","journal-title":"Asociaci\u00f3n Cubana de Producci\u00f3n Animal"},{"key":"S0022149X15001029_ref23","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2001.01284.x"},{"key":"S0022149X15001029_ref7","doi-asserted-by":"crossref","first-page":"429","DOI":"10.4269\/ajtmh.2003.69.429","article-title":"Hyperendemic fascioliasis associated with schistosomiasis villages in the Nile Delta of Egypt","volume":"69","author":"Esteban","year":"2003","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0022149X15001029_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.10.010"},{"key":"S0022149X15001029_ref36","first-page":"152","volume-title":"Sustaining the drive to overcome the global impact of neglected tropical diseases: second WHO report on neglected tropical diseases","year":"2013"},{"key":"S0022149X15001029_ref21","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3464-6"},{"key":"S0022149X15001029_ref35","unstructured":"WHO (2007) The \u2018neglected\u2019 neglected worms. Actions Against Worms Newsletter December, 1\u20138."},{"key":"S0022149X15001029_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(09)69002-3"},{"key":"S0022149X15001029_ref27","doi-asserted-by":"publisher","DOI":"10.1111\/jeb.12174"},{"key":"S0022149X15001029_ref28","doi-asserted-by":"publisher","DOI":"10.7208\/chicago\/9780226797670.001.0001"},{"key":"S0022149X15001029_ref12","unstructured":"Goudet J. (2001) FSTAT, a program to estimate and test gene diversities and fixation indices. Available at http:\/\/www.unil.ch\/izea\/softwares\/fstat.html (accessed accessed 19 May 2012)."},{"key":"S0022149X15001029_ref13","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X10000350"},{"key":"S0022149X15001029_ref2","first-page":"381","article-title":"Bridging gaps in the molecular phylogeny of the Lymnaeidae (Gastropoda: Pulmonata), vectors of fascioliasis","volume":"10","author":"Correa","year":"2010","journal-title":"Evolutionary Biology"},{"key":"S0022149X15001029_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2009.10.005"},{"key":"S0022149X15001029_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.01.009"},{"key":"S0022149X15001029_ref19","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001007715"},{"key":"S0022149X15001029_ref20","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/j.0014-3820.2001.tb00633.x","article-title":"Genetic variation in a host\u2013parasite association: potential for coevolution and frequency-dependent selection","volume":"55","author":"Johachim","year":"2001","journal-title":"Evolution"},{"key":"S0022149X15001029_ref9","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1807-0"},{"key":"S0022149X15001029_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.03.014"},{"key":"S0022149X15001029_ref11","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1996.0148"},{"key":"S0022149X15001029_ref8","unstructured":"Estoup A. & Martin O. (1996) Marqueurs microsatellites: isolement \u00e0 l'aide de sondes non-radioactives, caract\u00e9rization et mise au point. Available at http:\/\/www.inapg.inra.fr\/dsa\/microsat\/microsat.htm (accessed accessed 23 September 2004)."},{"key":"S0022149X15001029_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.07.022"},{"key":"S0022149X15001029_ref18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2004.00786.x"},{"key":"S0022149X15001029_ref14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.9.3566"},{"key":"S0022149X15001029_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.05.026"},{"key":"S0022149X15001029_ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2435.2010.01753.x"},{"key":"S0022149X15001029_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01442-3"},{"key":"S0022149X15001029_ref25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1989.tb04220.x"},{"key":"S0022149X15001029_ref1","unstructured":"Arnaud-Haond S. & Belkhir K. (2007) GenClone 2.0: a computer program to analyse genotypic data, test for clonality and describe spatial clonal organization. Available at http:\/\/si-wagner.ualg.pt\/ccmar\/maree\/software.php?soft\u00a0=\u00a0genclon (accessed accessed 20 July 2015)."},{"key":"S0022149X15001029_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.07.003"},{"key":"S0022149X15001029_ref33","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X13000382"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X15001029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,18]],"date-time":"2019-04-18T17:05:45Z","timestamp":1555607145000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X15001029\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,14]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["S0022149X15001029"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x15001029","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,14]]}}}