{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:07:51Z","timestamp":1790644071852,"version":"4.1.0"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2016,5,6]],"date-time":"2016-05-06T00:00:00Z","timestamp":1462492800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Trichinella<\/jats:italic>species are zoonotic nematodes that infect wild carnivores and omnivores throughout the world. We examined the prevalence and species of<jats:italic>Trichinella<\/jats:italic>infections in cougars (<jats:italic>Puma concolor couguar<\/jats:italic>) from Colorado, United States. Tongues from cougars were examined by pepsin\u2013HCl artificial digestion to detect<jats:italic>Trichinella<\/jats:italic>spp. larvae. The species or genotype of individual worms was identified by multiplex polymerase chain reaction (PCR).<jats:italic>Trichinella<\/jats:italic>spp. larvae were detected in 17 of 39 cougars (43.6% (28.7\u201359.5%)). Five of the cougars (12.8%) were infected with<jats:italic>T. murrelli<\/jats:italic>, 3 (7.7%) were infected with<jats:italic>T. pseudospiralis<\/jats:italic>, and 1 (2.6%) had<jats:italic>Trichinella<\/jats:italic>genotype T6.<jats:italic>Trichinella<\/jats:italic>spp. larvae from eight cougars were not identified at the species level, due to degraded DNA. The high prevalence of<jats:italic>Trichinella<\/jats:italic>spp. in cougars from Colorado and reports of the parasite in other populations of<jats:italic>Puma<\/jats:italic>spp. suggest that this large predator is a key mammalian reservoir.<\/jats:p>","DOI":"10.1017\/s0022149x16000262","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T10:19:42Z","timestamp":1462961982000},"page":"320-325","source":"Crossref","is-referenced-by-count":18,"title":["The occurrence of<i>Trichinella<\/i>species in the cougar<i>Puma concolor couguar<\/i>from the state of Colorado and other regions of North and South America"],"prefix":"10.1017","volume":"91","author":[{"given":"M.V.","family":"Reichard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Logan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Criffield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.E.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Paritte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.M.","family":"Messerly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Interisano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Marucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Pozio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2016,5,6]]},"reference":[{"key":"S0022149X16000262_ref17","first-page":"22","article-title":"Sylvatic trichinosis in carnivorous mammals in the Rocky Mountain region of Colorado","volume":"46","author":"Olsen","year":"1960","journal-title":"Journal of Parasitology"},{"key":"S0022149X16000262_ref7","unstructured":"ESA (Endangered Species Act). 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