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Helminthol."],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this paper was to evaluate the genetic relationship between two isolates of <jats:italic>Opisthorchis<\/jats:italic> (<jats:italic>O. pedicellata<\/jats:italic> sp. 1, <jats:italic>O. pedicellata<\/jats:italic> sp. 2) from two siluroid fish hosts, based on three nuclear DNA (18S, internal transcribed spacer 1 (ITS1) and ITS2). Molecular analysis revealed that both isolates of 18S sequences showed low intraspecific variability (1.6%), while this was 1.9% for ITS1 and 2.6% for ITS2. The secondary structure of the ITS2 region exhibited a remarkable four-helix model, with helices 1 and 4 being relatively short, three U\u2013U mismatches in helix 2 and the longest helix (3) having two UGGG motifs. The phylogenetic analyses, using neighbour\u2013joining (NJ) and maximum parsimony (MP) methods of MEGA 6, demonstrate that both isolates form a sister clade with significant support (100%). Therefore, we concluded that both the isolates of <jats:italic>O. pedicellata<\/jats:italic> are of the same species but some variations may be due to the sympatric environment of variant hosts.<\/jats:p>","DOI":"10.1017\/s0022149x16000390","type":"journal-article","created":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T22:20:42Z","timestamp":1466634042000},"page":"302-311","source":"Crossref","is-referenced-by-count":4,"title":["Ribosomal DNA analysis of isolates of the liver fluke <i>Opisthorchis pedicellata<\/i> (Verma, 1927) from two siluroid fish species in India"],"prefix":"10.1017","volume":"91","author":[{"given":"K.","family":"Choudhary","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Agrawal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,6,22]]},"reference":[{"key":"S0022149X16000390_ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3616-8"},{"key":"S0022149X16000390_ref32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm827"},{"key":"S0022149X16000390_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(96)82612-9"},{"key":"S0022149X16000390_ref7","doi-asserted-by":"publisher","DOI":"10.1006\/mpev.2001.0938"},{"key":"S0022149X16000390_ref11","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.7.2932-2941.2001"},{"key":"S0022149X16000390_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(03)00118-5"},{"key":"S0022149X16000390_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2006.09.019"},{"key":"S0022149X16000390_ref15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1986.tb05751.x"},{"key":"S0022149X16000390_ref40","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"},{"key":"S0022149X16000390_ref21","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp966"},{"key":"S0022149X16000390_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90042-W"},{"key":"S0022149X16000390_ref16","doi-asserted-by":"publisher","DOI":"10.1086\/417338"},{"key":"S0022149X16000390_ref35","first-page":"2725","article-title":"MEGA6: 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