{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T20:56:26Z","timestamp":1774990586180,"version":"3.50.1"},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T00:00:00Z","timestamp":1473897600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sparganosis is one of the top three tissue-dwelling heterologous helminthic diseases, along with cysticercosis and paragonimiasis, in Korea. Due to a lack of effective early diagnosis and treatment methods, this parasitic disease is regarded as a public health threat. This study evaluated reactivity, against sparganum extracts, of sera from inhabitants of Cheorwon-gun, Goseong-gun and Ongjin-gun in Korea. The sera from 836 subjects were subjected to enzyme-linked immunosorbent assay and immunoblot analysis. The sera from 18 (5.8%) and 15 (5.1%) inhabitants in Cheorwon-gun (<jats:italic>n<\/jats:italic> = 312) and Goseong-gun (<jats:italic>n<\/jats:italic> = 294), respectively, exhibited highly positive reactions to the sparganum antigen, whereas only two (0.9%) inhabitants in Ongjin-gun (<jats:italic>n<\/jats:italic> = 230) showed positivity. We sought antigenic proteins for serodiagnosis of positive sera by immunoproteomic approaches. Total sparganum lysates were separated by two-dimensional electrophoresis and then subjected to immunoblot analysis with mixed sparganosis-positive sera. We found seven antigenic spots and identified paramyosin as an antigenic protein by liquid chromatography\u2013mass spectrometry. By two-dimensional (2D)-based mass analysis and immunoblotting against sparganosis-positive sera, paramyosin was identified as a candidate antigen for serodiagnosis of sparganosis.<\/jats:p>","DOI":"10.1017\/s0022149x16000584","type":"journal-article","created":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T07:37:06Z","timestamp":1473925026000},"page":"642-646","source":"Crossref","is-referenced-by-count":1,"title":["Seropositivity and identification of paramyosin for sparganosis in the Kangwon and Incheon provinces of the Republic of Korea"],"prefix":"10.1017","volume":"91","author":[{"given":"M.-R.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.-W.","family":"Ju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.-W.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.-S.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.-Y.","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.-H.","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,9,15]]},"reference":[{"key":"S0022149X16000584_ref20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1996.d01-4.x"},{"key":"S0022149X16000584_ref18","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2006.44.2.151"},{"key":"S0022149X16000584_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S0022149X16000584_ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2206-2"},{"key":"S0022149X16000584_ref15","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-368"},{"key":"S0022149X16000584_ref12","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700613"},{"key":"S0022149X16000584_ref11","first-page":"576","article-title":"Identification 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analysis of the excretory\u2013secretory proteins from Spirometra mansoni sparganum","volume":"8","author":"Dan","year":"2013","journal-title":"Iranian Journal of Parasitology"},{"key":"S0022149X16000584_ref2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0178"}],"container-title":["Journal of 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