{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T23:11:25Z","timestamp":1720912285173},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human gnathostomiasis is a food-borne zoonosis caused by a tissue nematode of the genus <jats:italic>Gnathostoma<\/jats:italic>. The disease is highly endemic in Asia, including Thailand. The freshwater swamp eel (<jats:italic>Monopterus albus<\/jats:italic>), the second intermediate host of the gnathostome nematode, has an important role in transmitting the infection in Thailand. Surveys on the infective larvae of <jats:italic>Gnathostoma spinigerum<\/jats:italic> based on morphological features in freshwater swamp eels have been performed continuously and reported in Thailand. However, there is still limited molecular data on intra-species variations of the parasite. In this study, a total of 19 third-stage larvae of morphologically identified <jats:italic>G. spinigerum<\/jats:italic> were collected from 437 liver samples of freshwater swamp eels purchased from a large wholesale market in Bangkok, Thailand. Molecular characterization based on mitochondrial cytochrome <jats:italic>c<\/jats:italic> oxidase subunit I (COI) sequences was performed to elucidate their genetic variations and phylogenetic relationship. Among the 19 infective larvae recovered from these eels, 16 were sequenced successfully. Phylogenetic analyses inferred from the partial COI gene showed the presence of three distinct COI haplotypes. Our findings confirm the presence of <jats:italic>G. spinigerum<\/jats:italic> as the main species in Thailand.<\/jats:p>","DOI":"10.1017\/s0022149x16000857","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T04:37:02Z","timestamp":1480307822000},"page":"767-771","source":"Crossref","is-referenced-by-count":4,"title":["Genetic diversity of infective larvae of <i>Gnathostoma spinigerum<\/i> (Nematoda: Gnathostomatidae) in freshwater swamp eels from Thailand"],"prefix":"10.1017","volume":"91","author":[{"given":"P.","family":"Eamsobhana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Wanachiwanawin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Roongruangchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.L.","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.S.","family":"Yong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,11,28]]},"reference":[{"key":"S0022149X16000857_ref2","first-page":"887","article-title":"A case of cutaneous larva migrans caused by Gnathostoma spinigerum","volume":"53","author":"Aozasa","year":"2011","journal-title":"Hifu(ka No Rinsho"},{"key":"S0022149X16000857_ref8","first-page":"405","volume-title":"Gnathostoma","author":"Nawa","year":"2015"},{"key":"S0022149X16000857_ref7","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2016.002"},{"key":"S0022149X16000857_ref1","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005315"},{"key":"S0022149X16000857_ref11","first-page":"148","article-title":"Seasonal variation in the intensity of Gnathostoma larvae in swamp eels (Fluta alba) sold in a local market in Bangkok","volume":"29","author":"Rojekittikhun","year":"1988","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X16000857_ref4","volume-title":"A monograph on the genus Gnathostoma and gnathostomiasis in Thailand","author":"Daengsvang","year":"1980"},{"key":"S0022149X16000857_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2004.12.002"},{"key":"S0022149X16000857_ref15","first-page":"220","article-title":"Gnathostomiasis in Thailand: a survey on intermediate hosts of Gnathostoma spp. with special reference to a new type of larvae found in Fluta alba","volume":"22","author":"Setasuban","year":"1991","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X16000857_ref10","first-page":"144","article-title":"Gnathostome infection in swamp eels, Fluta alba, in central Thailand","volume":"29","author":"Nuamtanong","year":"1998","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X16000857_ref6","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0284"},{"key":"S0022149X16000857_ref3","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026036"},{"key":"S0022149X16000857_ref5","first-page":"124","volume-title":"(Ed.) The 25th Anniversary of the Faculty of Tropical Medicine, Mahidol University","author":"Daengsvang","year":"1986"},{"key":"S0022149X16000857_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.04.020"},{"key":"S0022149X16000857_ref13","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2012.50.2.113"},{"key":"S0022149X16000857_ref14","doi-asserted-by":"publisher","DOI":"10.1179\/000349802125000295"},{"key":"S0022149X16000857_ref16","first-page":"133","article-title":"A survey of infective larvae of Gnathostoma in eels sold in Ho Chi Minh City","volume":"31","author":"Xuan","year":"2000","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X16000857_ref12","first-page":"7","article-title":"A survey on the infective larvae of Gnathostoma spinigerum in freshwater fish sold in the local markets of Bangkok","volume":"12","author":"Rojekittikhun","year":"1989","journal-title":"Journal of Tropical Medicine and Parasitology"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X16000857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T15:57:31Z","timestamp":1555430251000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X16000857\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,28]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["S0022149X16000857"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x16000857","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,28]]}}}