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In addition, lymnaeid snails collected from the study area were examined microscopically for developmental stages of<jats:italic>Fasciola<\/jats:italic>spp. Abattoir inspection revealed that 51 out of 458 cattle livers (11.1%) contained adult flukes, which were identified morphologically as<jats:italic>Fasciola hepatica.<\/jats:italic>Examination of the cattle faecal samples revealed that 142 out of 503 (28.2%) contained<jats:italic>Fasciola<\/jats:italic>eggs. The collected snails, identified as<jats:italic>Galba truncatula<\/jats:italic>and<jats:italic>Radix natalensis,<\/jats:italic>showed larval stages of<jats:italic>Fasciola<\/jats:italic>in 71 out of 731 (9.7%)<jats:italic>G. truncatula<\/jats:italic>, while<jats:italic>R. natalensis<\/jats:italic>showed no infection. Specific duplex polymerase chain reaction (PCR) targeting the mitochondrial<jats:italic>cox1<\/jats:italic>gene of<jats:italic>F. hepatica<\/jats:italic>and<jats:italic>Fasciola gigantica<\/jats:italic>was carried out on DNA extracted from pooled infected snails and adult worms. The<jats:italic>F. hepatica<\/jats:italic>size amplicon (1031 bp) was obtained from both the infected<jats:italic>G. truncatula<\/jats:italic>and the adult worms isolated from cattle livers from different districts. The amplicon sequences were identical to the published sequences of<jats:italic>F. hepatica<\/jats:italic>mitochondrial<jats:italic>cox1<\/jats:italic>gene. In conclusion, the zoonotic importance of<jats:italic>Fasciola<\/jats:italic>infection and appropriate hygienic measures must be taken into consideration in Dakhla Oasis, Egypt.<\/jats:p>","DOI":"10.1017\/s0022149x17000086","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T09:30:53Z","timestamp":1486373453000},"page":"56-63","source":"Crossref","is-referenced-by-count":13,"title":["<i>Fasciola hepatica<\/i>infections in cattle and the freshwater snail<i>Galba truncatula<\/i>from Dakhla Oasis, Egypt"],"prefix":"10.1017","volume":"92","author":[{"given":"W.M.","family":"Arafa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.I.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.A.M.","family":"Snousi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kh.M.","family":"El-Dakhly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.J.","family":"Holman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.M.","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.M.","family":"Aboelhadid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"S0022149X17000086_ref8","first-page":"2","article-title":"Economic losses from fascioliasis in slaughtered animals: at abattoir levels","volume":"5","author":"Atallah","year":"2008","journal-title":"Minufiya Veterinary Journal"},{"key":"S0022149X17000086_ref43","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0429"},{"key":"S0022149X17000086_ref50","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.260"},{"key":"S0022149X17000086_ref20","unstructured":"Diab, M.R. 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