{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:52:23Z","timestamp":1761490343624},"reference-count":11,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T00:00:00Z","timestamp":1500854400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Urocanic acid (UCA) is known as a major chemoattractant for <jats:italic>Strongyloides stercoralis<\/jats:italic> infective third-stage larvae (L3). Since <jats:italic>Brugia pahangi<\/jats:italic> is a skin-penetrating parasitic nematode similar to <jats:italic>S. stercoralis<\/jats:italic>, UCA was expected to be a chemoattractant for <jats:italic>B. pahangi<\/jats:italic> L3. Thus, the chemoattractant activity of UCA for <jats:italic>B. pahangi<\/jats:italic> L3 was assessed. The chemotactic responses of <jats:italic>B. pahangi<\/jats:italic> L3 to UCA or acetic acid (CH<jats:sub>3<\/jats:sub>COOH) dissolved in amine solutions were assessed using an agar-plate assay. A test solution of 200 m<jats:sc>m<\/jats:sc> UCA dissolved in aqueous 270 m<jats:sc>m<\/jats:sc> tris(hydroxymethyl)aminomethane (Tris) significantly attracted <jats:italic>B. pahangi<\/jats:italic> L3 compared with deionized water (DW), while neither a solution of 200 m<jats:sc>m<\/jats:sc> UCA dissolved in aqueous 230 m<jats:sc>m<\/jats:sc> ammonia (NH<jats:sub>3<\/jats:sub>) nor 290 m<jats:sc>m<\/jats:sc> triethylamine (TEA) significantly attracted L3. Similarly, a test solution of 200 m<jats:sc>m<\/jats:sc> CH<jats:sub>3<\/jats:sub>COOH dissolved with 200 m<jats:sc>m<\/jats:sc> Tris significantly attracted L3, but neither a test solution of 200 m<jats:sc>m<\/jats:sc> CH<jats:sub>3<\/jats:sub>COOH plus 200 m<jats:sc>m<\/jats:sc> NH<jats:sub>3<\/jats:sub> nor 200 m<jats:sc>m<\/jats:sc> TEA attracted L3. Furthermore, L3 were significantly attracted to 200 m<jats:sc>m<\/jats:sc> Tris alone, compared with DW, but avoided 200 m<jats:sc>m<\/jats:sc> NH<jats:sub>3<\/jats:sub> and 200 m<jats:sc>m<\/jats:sc> TEA. Moreover, the chemoattractant activity of Tris for L3 was observed even at a low concentration of 25 m<jats:sc>m<\/jats:sc>, and it was observed in a mild alkaline condition but not in an acidic condition. The present study reveals that Tris is a potential chemoattractant for <jats:italic>B. pahangi<\/jats:italic> L3 while UCA is not. This finding will contribute to an understanding of the mechanisms of skin-penetrating infection of filarial L3.<\/jats:p>","DOI":"10.1017\/s0022149x17000645","type":"journal-article","created":{"date-parts":[[2017,7,23]],"date-time":"2017-07-23T23:59:31Z","timestamp":1500854371000},"page":"317-321","source":"Crossref","is-referenced-by-count":4,"title":["Chemoattractant activity of tris-(hydroxymethyl)aminomethane for <i>Brugia pahangi<\/i> infective third-stage larvae"],"prefix":"10.1017","volume":"92","author":[{"given":"Y.","family":"Mitsui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.E.","family":"Ure\u00f1a-Tatis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Aoki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,7,24]]},"reference":[{"key":"S0022149X17000645_ref11","doi-asserted-by":"publisher","DOI":"10.1080\/01621459.1927.10502953"},{"key":"S0022149X17000645_ref9","doi-asserted-by":"publisher","DOI":"10.1007\/BF00928357"},{"key":"S0022149X17000645_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5769(00)00039-8"},{"key":"S0022149X17000645_ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610193104"},{"key":"S0022149X17000645_ref6","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X11000605"},{"key":"S0022149X17000645_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2007.12.006"},{"key":"S0022149X17000645_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(67)90060-0"},{"key":"S0022149X17000645_ref1","first-page":"252","volume-title":"Biostatistics: A foundation for analysis in the health sciences","author":"Daniel","year":"1999"},{"key":"S0022149X17000645_ref10","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927408"},{"key":"S0022149X17000645_ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-0838-1"},{"key":"S0022149X17000645_ref3","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182003003433"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X17000645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,14]],"date-time":"2019-04-14T17:00:49Z","timestamp":1555261249000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X17000645\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,24]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["S0022149X17000645"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x17000645","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,24]]}}}