{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:53:20Z","timestamp":1777442000024,"version":"3.51.4"},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T00:00:00Z","timestamp":1507075200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Schistosomiasis \u2013 caused by trematodes from the genus<jats:italic>Schistosoma<\/jats:italic>\u2013 affects more than 200 million people worldwide. Growing resistance to therapy with praziquantel (PZQ) has encouraged the search for novel treatments against this neglected disease. The compound 7-epiclusianone (7-epi) \u2013 isolated from \u2018bacupari\u2019 (the fruit of the<jats:italic>Gracinia brasiliensis<\/jats:italic>tree) \u2013 has promising activity against<jats:italic>Schistosoma mansoni in vitro<\/jats:italic>, damaging the parasite's tegument. However, the target and mechanism of action of 7-epi have not been identified. Here, we examined the possibility that 7-epi harms the tegument by inhibiting parasite superoxide dismutase (SOD), which protects the tegument from damage by reactive oxygen species produced by host immune cells. Molecular docking analysis<jats:italic>in silico<\/jats:italic>suggested strong interactions between 7-epi and<jats:italic>S. mansoni<\/jats:italic>cytosolic superoxide dismutase (SmCtSOD) at allosteric cavities.<jats:italic>Schistosoma mansoni<\/jats:italic>couples were cultivated<jats:italic>ex vivo<\/jats:italic>with 12.44\u2013198.96 \u03bc<jats:sc>m<\/jats:sc>7-epi for 24 h, and then parasite extracts were tested for lipid peroxidation (as a surrogate for oxidative stress), and SOD activity and expression. Lipid peroxidation levels increased after incubation with concentrations \u226599.48 \u03bc<jats:sc>m<\/jats:sc>7-epi, and this compound reduced SOD activity at concentrations \u226524.87 \u03bcm. However, contact with 7-epi did not alter SOD expression, by quantitative real-time polymerase chain reaction (qRT-PCR). Our results show that the inhibition of SmCtSOD is partly responsible for the tegument detachment observed after incubation with 7-epi, but is not the only cause of the antiparasitic action of this compound<jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0022149x17000700","type":"journal-article","created":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T09:56:59Z","timestamp":1507111019000},"page":"535-543","source":"Crossref","is-referenced-by-count":4,"title":["The natural compound 7-epiclusianone inhibits superoxide dismutase activity in<i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"92","author":[{"given":"M.S.","family":"Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.P.","family":"Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.T.","family":"de Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.M.M.","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.L.","family":"Martins-Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.A.","family":"Colombo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.C.","family":"Elias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.H.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.J.","family":"Marques","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,10,4]]},"reference":[{"key":"S0022149X17000700_ref40","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4670"},{"key":"S0022149X17000700_ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1996.d01-6.x"},{"key":"S0022149X17000700_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.01.007"},{"key":"S0022149X17000700_ref41","unstructured":"World Health Organization (2016) Schistosomiasis. 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