{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T21:32:14Z","timestamp":1781991134899,"version":"3.54.5"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T00:00:00Z","timestamp":1509926400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cystic echinococcosis (CE) is a medically important parasite-caused human disease. Humans may acquire the infection accidentally by ingestion of <jats:italic>E. granulosus<\/jats:italic> eggs. The parasite has a broad range of hosts and genotypes, which may affect its aetiological and biological characteristics. The present study aimed to determine the genetic characteristics of human isolates of <jats:italic>E. granulosus<\/jats:italic> in Isfahan, Iran. In this cross-sectional study, 50 surgically removed hydatid cysts were collected from hospitalized patients in Al-Zahra Hospital, Isfahan, Iran, over a period of 2 years (2015\u20132017). DNA was extracted from cyst material, and polymerase chain reactions (PCR) were performed targeting <jats:italic>cox1<\/jats:italic> and <jats:italic>nad1<\/jats:italic> genes. Amplicons were sequenced directly and the resulting sequences were aligned and analysed. Phylogenetic and genetic diversity analyses were also performed. Among the isolates, 43 (86%), 3 (6%) and 4 (8%) out of 50 were <jats:italic>E. granulosus<\/jats:italic> (G1), <jats:italic>E. granulosus<\/jats:italic> (G3) and <jats:italic>E. intermedius<\/jats:italic> (G6), respectively. In total, nine and eight haplotypes were identified by <jats:italic>nad1<\/jats:italic> and <jats:italic>cox1<\/jats:italic> gene analysis, respectively. The haplotype diversity index was higher by <jats:italic>cox1<\/jats:italic> gene analysis (0.547) in G1 strains compared with <jats:italic>nad1<\/jats:italic> (0.433). The G1 genotype was the most predominant isolate from human cases of CE, and the presence of G6 is indicative of an important role of camels in the development of human CE in Isfahan. This is the first report of the G3 genotype causing human CE in Isfahan. Moreover, <jats:italic>cox1<\/jats:italic> gene analysis enables a higher resolution of the genetic diversity of the <jats:italic>E. granulosus<\/jats:italic> population compared with <jats:italic>nad1<\/jats:italic> gene analysis.<\/jats:p>","DOI":"10.1017\/s0022149x17000967","type":"journal-article","created":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T03:57:27Z","timestamp":1509940647000},"page":"696-702","source":"Crossref","is-referenced-by-count":21,"title":["Genetic characterization of <i>Echinococcus granulosus<\/i> strains isolated from humans based on <i>nad1<\/i> and <i>cox1<\/i> gene analysis in Isfahan, central Iran"],"prefix":"10.1017","volume":"92","author":[{"given":"R.","family":"Jafari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Sanei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Baradaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Spotin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Bagherpour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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