{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:25:23Z","timestamp":1762298723975},"reference-count":32,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T00:00:00Z","timestamp":1511395200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Trichinella<\/jats:italic> species are widely distributed on all continents with the exception of Antarctica, although the full spectrum of <jats:italic>Trichinella<\/jats:italic> species found in sub-Saharan African countries, and their hosts, has not been fully documented. This study was conducted to determine the prevalence of <jats:italic>Trichinella<\/jats:italic> in wildlife from the Greater Kruger National Park (GKNP) and adjacent areas located in the Limpopo and Mpumalanga provinces of South Africa, and to identify the species and\/or genotypes of <jats:italic>Trichinella<\/jats:italic> larvae isolated from muscle tissues, using molecular techniques. A review of <jats:italic>Trichinella<\/jats:italic> spp. and their wildlife hosts reported during 1964\u20132011 was also conducted and the results were compared with our current study. Ninety samples representing 15 mammalian, two bird and three reptile species were screened for <jats:italic>Trichinella<\/jats:italic> infection during 2012\u20132016, using artificial digestion. Isolates detected were identified using a multiplex polymerase chain reaction (PCR) amplification of the internal transcriber spacers ITS1 and ITS2, and expansion segment V (ESV) regions of ribosomal DNA, followed by molecular analysis of the sequences. Twenty samples from seven wildlife species were positive for <jats:italic>Trichinella<\/jats:italic> spp. larvae, with an overall prevalence of 21.1% (20\/90). The prevalence was higher in carnivores (18.9%, 18\/90) than in omnivores (2.2%, 2\/90). Analysis of sequences showed that eight of the isolates \u2013 two from spotted hyaena (<jats:italic>Crocuta crocuta<\/jats:italic>) (2\/8), three from lion (<jats:italic>Panthera leo<\/jats:italic>) (3\/13), one from leopard (<jats:italic>Panthera pardus<\/jats:italic>) (1\/6), one from small spotted genet (<jats:italic>Genetta genetta<\/jats:italic>) (1\/2) and one Nile monitor lizard (<jats:italic>Varanus niloticus<\/jats:italic>) (1\/2) \u2013 conformed to <jats:italic>Trichinella zimbabwensis<\/jats:italic>. One isolate from a hyaena was grouped under the encapsulated species clade comprising <jats:italic>T. nelsoni<\/jats:italic> and genotype <jats:italic>Trichinella<\/jats:italic> T8 reported to be present in South Africa. This is the first report confirming natural infection by <jats:italic>T. zimbabwensis<\/jats:italic> in hyaena, leopard, genet and Nile monitor lizard, adding to the body of knowledge on the epidemiology of <jats:italic>Trichinella<\/jats:italic> infections in the Greater Kruger National Park of South Africa. Ten <jats:italic>Trichinella<\/jats:italic>-like larval isolates recovered after digestion from four wildlife species in this study (2012\u20132016) revealed inconclusive results due to DNA degradation resulting from poor storage or too few larvae for analysis, in comparison to 20 unidentified isolates from five wildlife species during the 1964\u20132011 period.<\/jats:p>","DOI":"10.1017\/s0022149x17001079","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T05:14:17Z","timestamp":1511414057000},"page":"50-56","source":"Crossref","is-referenced-by-count":10,"title":["Prevalence and molecular identification of <i>Trichinella<\/i> species isolated from wildlife originating from Limpopo and Mpumalanga provinces of South Africa"],"prefix":"10.1017","volume":"93","author":[{"given":"S.","family":"Mukaratirwa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.J.","family":"La 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