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In the present study, a simple and rapid filtration-based test, dot immune\u2013gold filtration assay (DIGFA) was developed using a partially purified antigen of<jats:italic>Gnathostoma<\/jats:italic>third-stage larvae (L3). A total of 180 serum samples were tested to evaluate the diagnostic potential of DIGFA for gnathostomiasis. The diagnostic sensitivity and specificity were 96.7% (29\/30) and 100% (25\/25), respectively. The cross-reactivity with sera from other helminthiasis patients ranged from 0 to 4%, with an average of 1.6% (2\/125). DIGFA using a partially purified L3 antigen was not only simple and rapid, but also more accurate than standard assays for the diagnosis of human gnathostomiasis. DIGFA may represent a promising tool for application in laboratories or in the field, without requiring any instrumentation.<\/jats:p>","DOI":"10.1017\/s0022149x17001080","type":"journal-article","created":{"date-parts":[[2017,11,16]],"date-time":"2017-11-16T14:00:53Z","timestamp":1510840853000},"page":"26-32","source":"Crossref","is-referenced-by-count":5,"title":["A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of 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