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Faecal samples from 160 local dogs were collected and hookworm positive samples processed to recover larvae for further molecular characterization. DNA was extracted from pools of larvae from individual samples (n = 66), which were analysed subsequently using two different molecular approaches, polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR coupled with Sanger sequencing. The PCR-RFLP technique detected only the presence of the ubiquitous<jats:italic>Ancylostoma caninum<\/jats:italic>in the 66 samples. However, by species-specific PCR coupled with Sanger sequencing we identified ten samples with<jats:italic>A. braziliense<\/jats:italic>, two with<jats:italic>Uncinaria stenocephala<\/jats:italic>and five with<jats:italic>A. ceylanicum<\/jats:italic>. Thus, all four known species of canine hookworms were identified in Morogoro, Tanzania. To our knowledge this is the first report of the detection of the presence of<jats:italic>U. stenocephala<\/jats:italic>and<jats:italic>A. ceylanicum<\/jats:italic>in Africa using molecular techniques. In addition to their veterinary importance, canine hookworms have zoonotic potential and are of public health concern.<\/jats:p>","DOI":"10.1017\/s0022149x18000263","type":"journal-article","created":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T08:21:36Z","timestamp":1522657296000},"page":"313-318","source":"Crossref","is-referenced-by-count":17,"title":["Molecular identification of zoonotic hookworm species in dog faeces from Tanzania"],"prefix":"10.1017","volume":"93","author":[{"given":"A.","family":"Merino-Tejedor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Nejsum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.M.","family":"Mkupasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.V.","family":"Johansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Olsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2018,4,2]]},"reference":[{"key":"S0022149X18000263_ref12","doi-asserted-by":"publisher","DOI":"10.1139\/z59-030"},{"key":"S0022149X18000263_ref42","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-91"},{"key":"S0022149X18000263_ref43","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-67"},{"key":"S0022149X18000263_ref23","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-88"},{"key":"S0022149X18000263_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.07.006"},{"key":"S0022149X18000263_ref21","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.4.378"},{"key":"S0022149X18000263_ref45","volume-title":"Coordinated use of anthelminthic drugs in control interventions: a manual for health professionals and programme managers","year":"2006"},{"key":"S0022149X18000263_ref1","first-page":"139","article-title":"A survey of helminths in domestic cats in the Pretoria area of Transvaal, Republic of South Africa. 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