{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:25:57Z","timestamp":1762507557298,"version":"3.40.5"},"reference-count":24,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T00:00:00Z","timestamp":1540512000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Dawestrema cycloancistrium<\/jats:italic> is the main ectoparasite causing mortality in fingerlings of pirarucu (<jats:italic>Arapaima gigas<\/jats:italic>) in Amazonian aquaculture. Very little is known about the <jats:italic>D. cycloancistrium<\/jats:italic> reproductive index and appropriate methods of collecting eggs for investigation. This study aimed to determine the oviposition rate of <jats:italic>D. cycloancistrium<\/jats:italic>. To achieve this aim, two egg quantification methods were tested: the estimative method (ME) and the total counting method (MT). Compared with the MT, the ME overestimated the number of eggs counted, which were 2943.5 \u00b1 2840.6 and 1041.5 \u00b1 533 eggs, and the oviposition rate, which was 80.1 \u00b1 58.7 and 31.4 \u00b1 16.4 eggs\/parasite\/day, for ME and MT, respectively. These results show that for studies quantifying <jats:italic>D. cycloancistrium<\/jats:italic> eggs, the total eggs in the sample must be counted, as the estimates made using subsamples are not representative. Using the MT, the oviposition rate for <jats:italic>D. cycloancistrium<\/jats:italic> was determined to be 31.4 \u00b1 16.4 eggs per adult parasite per day. The present study demonstrates the egg production capacity of a monogenean species parasite of <jats:italic>A. gigas<\/jats:italic>, providing basic biological data for <jats:italic>D. cycloancistrium<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0022149x18000901","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T06:07:43Z","timestamp":1540534063000},"source":"Crossref","is-referenced-by-count":6,"title":["Methods for quantifying eggs and oviposition rate of Dawestrema cycloancistrium (Monogenea: Dactylogyridae), monogenean parasite of Arapaima gigas (Teleostei: Osteoglossidae)"],"prefix":"10.1017","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4151-6885","authenticated-orcid":false,"given":"P.O.","family":"Maciel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7369-9391","authenticated-orcid":false,"given":"R.R.","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2018,10,26]]},"reference":[{"key":"S0022149X18000901_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s11160-017-9490-1"},{"key":"S0022149X18000901_ref20","doi-asserted-by":"publisher","DOI":"10.1590\/1519-6984.14014"},{"key":"S0022149X18000901_ref15","first-page":"1","volume":"69","year":"2016","journal-title":"Journal of Statistical Software"},{"key":"S0022149X18000901_ref9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108801"},{"key":"S0022149X18000901_ref14","first-page":"321","volume":"98","year":"1985","journal-title":"Proceedings of the Biological Society of Washington"},{"key":"S0022149X18000901_ref8","doi-asserted-by":"publisher","DOI":"10.24841\/fa.v16i1-2.280"},{"key":"S0022149X18000901_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60344-9"},{"volume-title":"South American Monogenoidea Parasites of Fishes, Amphibians and Reptiles","year":"2013","key":"S0022149X18000901_ref7"},{"key":"S0022149X18000901_ref12","doi-asserted-by":"publisher","DOI":"10.1590\/1809-43922001312316"},{"key":"S0022149X18000901_ref6","first-page":"171","volume":"14","year":"1994","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"S0022149X18000901_ref5","doi-asserted-by":"publisher","DOI":"10.3354\/dao003051"},{"key":"S0022149X18000901_ref10","doi-asserted-by":"publisher","DOI":"10.1111\/are.12541"},{"key":"S0022149X18000901_ref4","doi-asserted-by":"publisher","DOI":"10.1007\/BF00932717"},{"key":"S0022149X18000901_ref3","first-page":"557","volume":"83","year":"1997","journal-title":"Journal of Parasitology"},{"volume":"67","journal-title":"Journal of Statistical Software","year":"2016","key":"S0022149X18000901_ref2"},{"key":"S0022149X18000901_ref1","first-page":"499","volume":"79","year":"2009","journal-title":"Veterinarski Arhiv"},{"key":"S0022149X18000901_ref27","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/icr003"},{"volume-title":"Reprodu\u00e7\u00e3o e engorda do pirarucu: levantamento de processos produtivos e tecnologias","year":"2015","key":"S0022149X18000901_ref26"},{"volume-title":"R: A Language and Environment for Statistical Computing","year":"2018","key":"S0022149X18000901_ref25"},{"key":"S0022149X18000901_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2007.11.013"},{"key":"S0022149X18000901_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00338-1"},{"key":"S0022149X18000901_ref22","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182008004691"},{"key":"S0022149X18000901_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2004.11.029"},{"key":"S0022149X18000901_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2017.09.022"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X18000901","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T02:58:58Z","timestamp":1571626738000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X18000901\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,26]]},"references-count":24,"alternative-id":["S0022149X18000901"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x18000901","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"type":"print","value":"0022-149X"},{"type":"electronic","value":"1475-2697"}],"subject":[],"published":{"date-parts":[[2018,10,26]]},"article-number":"e4"}}