{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T10:50:43Z","timestamp":1782903043436,"version":"3.54.5"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T00:00:00Z","timestamp":1540771200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enterobiasis, caused by the nematode <jats:italic>Enterobius vermicularis<\/jats:italic>, is a common health problem among schoolchildren in Thailand. We provide the first molecular identification of this nematode from Thai schoolchildren and document genetic variation among <jats:italic>E. vermicularis<\/jats:italic> eggs using sequence analyses of the mitochondrial cytochrome <jats:italic>c<\/jats:italic> oxidase subunit 1 (<jats:italic>cox<\/jats:italic>1) gene and the nuclear ribosomal DNA second internal transcribed spacer (ITS2). A cross-sectional parasitological survey was conducted in schoolchildren (n = 491) in five regions of Thailand between May 2015 and December 2016. The diagnosis of <jats:italic>Enterobius<\/jats:italic> infection was made using the adhesive tape perianal swab technique. <jats:italic>Enterobius<\/jats:italic> eggs were recovered from 43 participants (8.75%). DNA was extracted from these eggs and the <jats:italic>cox<\/jats:italic>1 gene and partial ITS2 region amplified using the polymerase chain reaction (PCR). Nineteen amplified PCR products of the <jats:italic>cox<\/jats:italic>1 gene (441 bp) and 18 of the ITS2 region (623 bp) were subsequently sequenced. All sequences were identified as belonging to <jats:italic>E. vermicularis<\/jats:italic> based on database searches. Phylogenetic analysis and a median-joining network of available <jats:italic>E. vermicularis cox<\/jats:italic>1 sequences showed 66 haplotypes. We found haploclusters (types A and B) represented among the Thai sequences. Six haplotypes from Thailand fell into type A (of Nakano <jats:italic>et al.<\/jats:italic>, 2006) (along with sequences from Japan and Korea) and five haplotypes into type B (with sequences from Japan, Iran, Czech Republic, Greece, Denmark and Sudan). The overall haplotype diversity (Hd) was 0.9888. Transmission of worms with type B haplotypes from primates to humans in Asia or from humans in Europe possibly occurs in Thailand.<\/jats:p>","DOI":"10.1017\/s0022149x18000962","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T04:57:34Z","timestamp":1540789054000},"source":"Crossref","is-referenced-by-count":15,"title":["Genetic variation of Enterobius vermicularis among schoolchildren in Thailand"],"prefix":"10.1017","volume":"94","author":[{"given":"K.","family":"Tomanakan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Sanpool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Chamavit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Lulitanond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-8783","authenticated-orcid":false,"given":"P.M.","family":"Intapan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6726-3759","authenticated-orcid":false,"given":"W.","family":"Maleewong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2018,10,29]]},"reference":[{"key":"S0022149X18000962_ref31","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02454-10"},{"key":"S0022149X18000962_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0238-4"},{"key":"S0022149X18000962_ref19","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-015-0058-9"},{"key":"S0022149X18000962_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp187"},{"key":"S0022149X18000962_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00157-1"},{"key":"S0022149X18000962_ref16","doi-asserted-by":"publisher","DOI":"10.1515\/helm-2017-0042"},{"key":"S0022149X18000962_ref15","first-page":"1161","volume":"69","year":"2004","journal-title":"American 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