{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T04:33:30Z","timestamp":1751603610799,"version":"3.40.5"},"reference-count":47,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T00:00:00Z","timestamp":1565740800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human ascariasis is a neglected tropical disease of great relevance to public health and is considered the most frequent helminthiasis in poor regions. Accurately diagnosing this parasite has been challenging due to limitations of current diagnostic methods. Immunoglobulin Y (IgY) technology is a very effective alternative for the production of highly specific and profitable antibodies. This study aimed to produce and apply anti-<jats:italic>Ascaris suum<\/jats:italic>IgY antibodies in the immunodiagnosis of human ascariasis. Five immunizations comprising total saline extract from<jats:italic>A. suum<\/jats:italic>adult life forms were given at 14-day intervals to<jats:italic>Gallus gallus domesticus<\/jats:italic>hens of the Isa Brown line. Eggs and blood samples were collected weekly and fortnightly, respectively, to monitor the production of antibodies. The specificity of antibodies was confirmed by dot-blot, kinetic enzyme-linked immunosorbent assay (ELISA), avidity ELISA, immunoblotting and indirect immunofluorescence antibody tests. The application for disease diagnosis was performed through the detection of immune complexes in human serum samples by sandwich ELISA. Peaks of IgY anti-<jats:italic>A. suum<\/jats:italic>production occurred at weeks 6 and 8. IgY showed high avidity levels after the second dose of immunization, ranging from 64% to 93%, with a mean avidity index of 78.30%. Purified IgY recognized 12 bands of proteins from<jats:italic>A. suum<\/jats:italic>saline extract. Eggs, the uterine portion and cuticles of<jats:italic>A. suum<\/jats:italic>female adult are reactive in immunofluorescence. The detection of immune complexes showed diagnostic values of 80% sensitivity and 90% specificity. In conclusion, specific IgY have been shown to be a potential immunodiagnostic tool with promising future applications in human ascariasis.<\/jats:p>","DOI":"10.1017\/s0022149x19000701","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T08:41:44Z","timestamp":1565772104000},"source":"Crossref","is-referenced-by-count":11,"title":["Anti-<i>Ascaris suum<\/i>immunoglobulin Y as a novel biotechnological tool for the diagnosis of human ascariasis"],"prefix":"10.1017","volume":"94","author":[{"given":"C.A.","family":"Lopes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.S.","family":"de Faria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.E.N.","family":"de Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.P.","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.P.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.L.","family":"Bueno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.M.","family":"Rodrigues \u00c1vila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1.","family":"Ferreira J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-8511","authenticated-orcid":false,"given":"J.M.","family":"Costa-Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2019,8,14]]},"reference":[{"key":"S0022149X19000701_ref44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006114"},{"key":"S0022149X19000701_ref21","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X19000701_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.09.012"},{"key":"S0022149X19000701_ref4","first-page":"41","article-title":"Eine Einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben. Mededeel. Mith H. Geneesk. Batavia","author":"Baermann","year":"1917","journal-title":"Lab Weltevreden Feestbundel"},{"key":"S0022149X19000701_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2011.05.006"},{"key":"S0022149X19000701_ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-017-2505-z"},{"key":"S0022149X19000701_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.12.011"},{"key":"S0022149X19000701_ref34","first-page":"81","article-title":"Epidemiological and clinical features of soil-transmitted helminths","volume":"7","author":"Parija","year":"2017","journal-title":"Tropical Parasitology"},{"key":"S0022149X19000701_ref17","first-page":"493","article-title":"Antigenic relationships between the surface-exposed, secreted and somatic materials of the nematode parasites Ascaris lumbricoides, Ascaris suum, and Toxocara canis","volume":"75","author":"Kennedy","year":"1989","journal-title":"Clinical and Experimental Immunology"},{"key":"S0022149X19000701_ref2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000063988"},{"key":"S0022149X19000701_ref19","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i33.7507"},{"key":"S0022149X19000701_ref39","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-04488-9_3"},{"key":"S0022149X19000701_ref8","doi-asserted-by":"publisher","DOI":"10.1080\/17843286.2018.1436956"},{"key":"S0022149X19000701_ref16","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012001230"},{"key":"S0022149X19000701_ref37","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-37"},{"key":"S0022149X19000701_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90179-B"},{"key":"S0022149X19000701_ref31","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782014000100025"},{"key":"S0022149X19000701_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.01.178"},{"key":"S0022149X19000701_ref28","doi-asserted-by":"publisher","DOI":"10.11606\/issn.2176-7262.v30i2p234-242"},{"key":"S0022149X19000701_ref13","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.8.1970792"},{"key":"S0022149X19000701_ref40","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1994.tb08122.x"},{"key":"S0022149X19000701_ref9","first-page":"1355","article-title":"Ascaris lumbricoides, Ascaris suum or \u2018Ascaris lumbrisuum\u2019?","volume":"213","author":"Da Silva Alves","year":"2016","journal-title":"The Journal of Infectious Diseases"},{"key":"S0022149X19000701_ref20","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-food-022811-101137"},{"key":"S0022149X19000701_ref12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040391"},{"key":"S0022149X19000701_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.12.017"},{"key":"S0022149X19000701_ref27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2011.01283.x"},{"key":"S0022149X19000701_ref24","doi-asserted-by":"publisher","DOI":"10.5847\/wjem.j.issn.1920-8642.2014.03.001"},{"key":"S0022149X19000701_ref45","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5239-3"},{"key":"S0022149X19000701_ref25","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"The Journal of Biological Chemistry"},{"key":"S0022149X19000701_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2019.05.015"},{"key":"S0022149X19000701_ref30","doi-asserted-by":"publisher","DOI":"10.1186\/s12937-015-0067-3"},{"key":"S0022149X19000701_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.05.009"},{"key":"S0022149X19000701_ref26","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761919000100006"},{"key":"S0022149X19000701_ref29","first-page":"507","article-title":"Contribui\u00e7\u00e3o para o estudo do Strongyloides stercoralis e da estrongiloid\u00edase no Brasil","volume":"1","author":"Moraes","year":"1948","journal-title":"Revista de Sa\u00fade P\u00fablica"},{"key":"S0022149X19000701_ref22","doi-asserted-by":"publisher","DOI":"10.1080\/09540109809354966"},{"key":"S0022149X19000701_ref18","first-page":"86","article-title":"Laboratory diagnosis of soil transmitted helminthiasis","volume":"7","author":"Khurana","year":"2017","journal-title":"Tropical Parasitology"},{"key":"S0022149X19000701_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.01.007"},{"key":"S0022149X19000701_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80008-5"},{"key":"S0022149X19000701_ref33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004382"},{"key":"S0022149X19000701_ref7","doi-asserted-by":"publisher","DOI":"10.1385\/IR:21:1:1"},{"key":"S0022149X19000701_ref35","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00323"},{"key":"S0022149X19000701_ref38","doi-asserted-by":"publisher","DOI":"10.3109\/08830185.2013.863304"},{"key":"S0022149X19000701_ref3","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000000655"},{"key":"S0022149X19000701_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(01)00508-6"},{"key":"S0022149X19000701_ref42","doi-asserted-by":"publisher","DOI":"10.5604\/17331331.1197285"},{"key":"S0022149X19000701_ref47","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12325"},{"key":"S0022149X19000701_ref46","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00013274"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X19000701","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T18:04:22Z","timestamp":1610820262000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X19000701\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,14]]},"references-count":47,"alternative-id":["S0022149X19000701"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x19000701","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"type":"print","value":"0022-149X"},{"type":"electronic","value":"1475-2697"}],"subject":[],"published":{"date-parts":[[2019,8,14]]},"article-number":"e71"}}