{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:26:11Z","timestamp":1777418771284,"version":"3.51.4"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T00:00:00Z","timestamp":1579564800000},"content-version":"unspecified","delay-in-days":20,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Glutathione <jats:italic>S<\/jats:italic>-transferases (GSTs) are a detoxifying enzyme family that is essential for parasite blood-feeding and survival, and represent potential targets for hookworm vaccine development. Multiple GST-encoding complementary DNAs (cDNAs) have been cloned from <jats:italic>Ancylostoma caninum<\/jats:italic> and <jats:italic>Necator americanus<\/jats:italic>, but there are no reports about the cloning of this enzyme from <jats:italic>Ancylostoma ceylanicum<\/jats:italic>, the animal-derived zoonotic hookworm. To study the molecular nature and tissue localization of GST of <jats:italic>A. ceylanicum<\/jats:italic> (<jats:italic>Ace<\/jats:italic>-GST), we designed primers based on the GST gene sequence of <jats:italic>A. ceylanicum<\/jats:italic> in GenBank, amplified the <jats:italic>Ace<\/jats:italic>-GST cDNA by reverse transcription polymerase chain reaction, and analysed its homology and genetic evolution relationship. The amplified product was cloned into the pET-32a vector and transformed into <jats:italic>Escherichia coli<\/jats:italic> BL21 (DE3) for expression. To prepare anti-GST polyclonal antibodies, the recombinant protein was purified and used to immunize Kunming mice. The level of immunoglobulin G (IgG) antibody in the serum of immunized mice was detected by indirect enzyme-linked immunosorbent assay, and the <jats:italic>Ace<\/jats:italic>-GST localization in adult worm was determined using the immunofluorescence method. The results showed that the full-length cDNA encoding <jats:italic>Ace<\/jats:italic>-GST was 468 bp, which had the highest homology with <jats:italic>Ac<\/jats:italic>-GST-1 (60.1%) and clustered into one branch (<jats:italic>v<\/jats:italic>-class) with <jats:italic>Ac<\/jats:italic>-GST-1 and <jats:italic>Na<\/jats:italic>-GST-1 in a phylogenetic tree. Mice immunized with recombinant <jats:italic>Ace<\/jats:italic>-GST showed specific IgG antibody response. Immunolocalization revealed that natural <jats:italic>Ace<\/jats:italic>-GST is mainly located in the epidermis, muscle and intestine of the adult. These results may lay a foundation for further studies on the biological function of <jats:italic>Ace<\/jats:italic>-GST.<\/jats:p>","DOI":"10.1017\/s0022149x20000012","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T02:37:50Z","timestamp":1579574270000},"source":"Crossref","is-referenced-by-count":5,"title":["Molecular characterization and tissue localization of glutathione <i>S<\/i>-transferase from adult <i>Ancylostoma ceylanicum<\/i>"],"prefix":"10.1017","volume":"94","author":[{"given":"J.X.","family":"Hang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.M.I.","family":"Abuzeid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.Q.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.X.","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9393-1573","authenticated-orcid":false,"given":"G.Q.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,1,21]]},"reference":[{"key":"S0022149X20000012_ref29","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00848-09"},{"key":"S0022149X20000012_ref28","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6903-6911.2005"},{"key":"S0022149X20000012_ref27","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1795-8"},{"key":"S0022149X20000012_ref25","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2780-2790.2004"},{"key":"S0022149X20000012_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.05.001"},{"key":"S0022149X20000012_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2007.08.005"},{"key":"S0022149X20000012_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)01015-3"},{"key":"S0022149X20000012_ref26","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20181132"},{"key":"S0022149X20000012_ref13","doi-asserted-by":"publisher","DOI":"10.1021\/bi100567j"},{"key":"S0022149X20000012_ref17","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.22.6282"},{"key":"S0022149X20000012_ref5","doi-asserted-by":"publisher","DOI":"10.1017\/S003118209900517X"},{"key":"S0022149X20000012_ref1","first-page":"343","article-title":"Efficacy of mebendazole and levamisole alone or in combination against intestinal nematode infections after repeated targeted mebendazole treatment in Zanzibar","volume":"81","author":"Albonico","year":"2003","journal-title":"Bulletin of the World Health Organization"},{"key":"S0022149X20000012_ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0020067"},{"key":"S0022149X20000012_ref20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13056"},{"key":"S0022149X20000012_ref9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.45.120403.095857"},{"key":"S0022149X20000012_ref8","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200600148X"},{"key":"S0022149X20000012_ref15","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/868050"},{"key":"S0022149X20000012_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2010.01.005"},{"key":"S0022149X20000012_ref3","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000061369"},{"key":"S0022149X20000012_ref4","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1067"},{"key":"S0022149X20000012_ref11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000053"},{"key":"S0022149X20000012_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01174-9"},{"key":"S0022149X20000012_ref14","doi-asserted-by":"publisher","DOI":"10.1126\/science.8424176"},{"key":"S0022149X20000012_ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001522"},{"key":"S0022149X20000012_ref16","first-page":"4","article-title":"The global burden of disease","volume":"106","author":"Murphy","year":"2013","journal-title":"Irish Medical Journal"},{"key":"S0022149X20000012_ref19","doi-asserted-by":"publisher","DOI":"10.3109\/03602532.2011.558093"},{"key":"S0022149X20000012_ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004246"},{"key":"S0022149X20000012_ref6","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000006454"},{"key":"S0022149X20000012_ref22","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf516"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X20000012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T02:38:48Z","timestamp":1579574328000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X20000012\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":29,"alternative-id":["S0022149X20000012"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x20000012","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]},"article-number":"e118"}}