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Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study describes two new species,<jats:italic>Trinigyrus anthus<\/jats:italic>n. sp. and<jats:italic>Trinigyrus carvalhoi<\/jats:italic>n. sp., from gills of<jats:italic>Hypostomus<\/jats:italic>spp. from the Upper Paran\u00e1 River basin, Brazil.<jats:italic>Trinigyrus peregrinus<\/jats:italic>is redescribed based on examination of its holotype, paratypes and new material of specimens parasitizing<jats:italic>Pterygoplichthys ambrosettii<\/jats:italic>, also from the Upper Paran\u00e1 River basin, Brazil. New morphological features were included in the diagnosis of the genus, such as the presence of a sclerotized border on the anchor base, and a weakly sclerotized fringe on the base of the male copulatory organ (MCO).<jats:italic>Trinigyrus anthus<\/jats:italic>n. sp. differs from other congeners by the shape of the MCO, presenting an enlarged base with sclerotized fringes resembling flower petals.<jats:italic>Trinigyrus carvalhoi<\/jats:italic>n. sp. and<jats:italic>T. peregrinus<\/jats:italic>are similar but can be differentiated from each other mainly by the sclerotization of the vagina (absent in the new species), and the morphology of the MCO (C-shaped versus one counterclockwise circle, respectively). For the first time, gene sequences of<jats:italic>Trinigyrus<\/jats:italic>spp. from Brazil were obtained (partial ribosomal 28S and mitochondrial cytochrome<jats:italic>c<\/jats:italic>oxidase I (mtCOI)). The genetic divergences among the new species and<jats:italic>T. peregrinus<\/jats:italic>varied from 2 to 3% (6\u201218\u00a0pb) based on sequences of 28S ribosomal DNA (rDNA), and 6\u20137% (83\u201292\u00a0pb) using mtCOI. Phylogenetic analyses based on partial 28S rDNA revealed that<jats:italic>Trinigyrus<\/jats:italic>,<jats:italic>Heteropriapulus<\/jats:italic>and<jats:italic>Unilatus<\/jats:italic>formed a monophyletic and well-supported clade of monogeneans from Neotropical freshwater loricariids, suggesting a closer relationship among these dactylogyrids and their hosts.<\/jats:p>","DOI":"10.1017\/s0022149x20000097","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T11:34:02Z","timestamp":1582198442000},"source":"Crossref","is-referenced-by-count":23,"title":["<i>Trinigyrus<\/i>spp. (Monogenea: Dactylogyridae) from Brazilian catfishes: new species, molecular data and new morphological contributions to the genus"],"prefix":"10.1017","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4733-4820","authenticated-orcid":false,"given":"L.","family":"Franceschini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1984-2414","authenticated-orcid":false,"given":"A.A.","family":"Acosta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6208-0286","authenticated-orcid":false,"given":"A.C.","family":"Zago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3407-8335","authenticated-orcid":false,"given":"M.I.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3426-6873","authenticated-orcid":false,"given":"R.J.","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,2,20]]},"reference":[{"key":"S0022149X20000097_ref52","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst064"},{"key":"S0022149X20000097_ref50","first-page":"1","article-title":"Phylogeography of Tetrancistrum nebulosi (Monogenea, Dactylogyridae) on the host of mottled spinefoot (Siganus fuscescens) in the South China Sea, inferred from mitochondrial COI and ND2 genes","volume":"27","author":"Wang","year":"2014","journal-title":"Mitochondrial DNA"},{"key":"S0022149X20000097_ref49","volume-title":"Amazon fish parasites","author":"Thatcher","year":"2006"},{"key":"S0022149X20000097_ref47","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2006.05.044"},{"key":"S0022149X20000097_ref22","doi-asserted-by":"publisher","DOI":"10.1023\/B:SYPA.0000029422.16712.9a"},{"key":"S0022149X20000097_ref41","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1365-2699.2007.01794.x","article-title":"Colonization of Pacific islands by parasites of low dispersal ability: phylogeography of two monogenean species parasitizing butterflyfishes in the South Pacific Ocean","volume":"35","author":"Plaisance","year":"2008","journal-title":"Journal of Biogeography"},{"key":"S0022149X20000097_ref20","volume-title":"Animal tissue techniques","author":"Humason","year":"1979"},{"key":"S0022149X20000097_ref35","doi-asserted-by":"publisher","DOI":"10.1109\/GCE.2010.5676129"},{"key":"S0022149X20000097_ref18","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"S0022149X20000097_ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-016-9671-7"},{"key":"S0022149X20000097_ref33","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0767-8"},{"key":"S0022149X20000097_ref48","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0022149X20000097_ref17","first-page":"113","article-title":"A review of Antarctic ichthyofaunas in the light of new fossil discoveries","volume":"29","author":"Grande","year":"1986","journal-title":"Palaeontology"},{"key":"S0022149X20000097_ref45","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg180"},{"key":"S0022149X20000097_ref11","first-page":"317","article-title":"Diversity of monogeneans parasites from Characiformes fishes in the Batalha river and Peixe's river, State of S\u00e3o Paulo, Brazil","volume":"11","author":"Dias","year":"2017","journal-title":"Neotropical Helminthology"},{"key":"S0022149X20000097_ref15","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X17000256"},{"key":"S0022149X20000097_ref3","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2019.004"},{"key":"S0022149X20000097_ref14","unstructured":"Eschmeyer, WN , Fricke, R and van der Laan, R (Eds) (2019) Catalog of fishes: Genera, species, references. 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