{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:27:58Z","timestamp":1770899278471,"version":"3.50.1"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"unspecified","delay-in-days":92,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Whipworm infection or trichuriasis caused by <jats:italic>Trichuris trichiura<\/jats:italic> is of major public health concern in sub-Saharan Africa, particularly among pre-school and school-going children. It is among the neglected tropical diseases targeted for elimination through mass drug administration (MDA). One of the outcomes of MDA is a rapid decline in levels of infection intensity, making it difficult to monitor effectiveness of control measures using the conventional Kato\u2013Katz procedure, which relies on the microscopic detection of parasite ova in faecal samples. In the present study, a loop-mediated isothermal amplification (LAMP) test was developed for the detection of <jats:italic>T. trichiura<\/jats:italic> infection in faecal samples. LAMP technology offers greater sensitivity and specificity than the microscopy-based tests. A set of four specific primers targeting the internal transcribed spacer 2 region of the ribosomal DNA were designed using Primer Explorer software. DNA was extracted from faecal samples using the alkaline lysis method (HotSHOT) and the LAMP reaction performed at 63\u00b0C for 1\u00a0h. The amplicons were visualized by both gel electrophoresis and with the naked eye following staining with SYBR green dye. Sensitivity and specificity tests were determined using the standard Kato\u2013Katz diagnostic procedure as a reference test. The developed LAMP assay reliably detected <jats:italic>T. trichiura<\/jats:italic> DNA in faecal samples, with a specificity and sensitivity of 88% and 77%, respectively. No cross-reactivity was observed with several common helminth parasites. The developed LAMP assay is an appropriate diagnostic method for the detection of <jats:italic>T. trichiura<\/jats:italic> DNA in human faecal samples due to its simplicity, low cost, high sensitivity and specificity.<\/jats:p>","DOI":"10.1017\/s0022149x2000022x","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T06:09:38Z","timestamp":1585807778000},"source":"Crossref","is-referenced-by-count":11,"title":["Development and evaluation of a loop-mediated isothermal amplification (LAMP) diagnostic test for detection of whipworm, <i>Trichuris trichiura<\/i>, in faecal samples"],"prefix":"10.1017","volume":"94","author":[{"given":"M.G.","family":"Ngari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.N.","family":"Mwangi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.P.","family":"Njoroge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kinyua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.A.","family":"Osuna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.M.","family":"Kimeu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.W.","family":"Okanya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7066-6770","authenticated-orcid":false,"given":"E.L.","family":"Agola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,4,2]]},"reference":[{"key":"S0022149X2000022X_ref23b","volume-title":"Preventive chemotherapy to control soil-transmitted helminth infections in at-risk population groups","year":"2017"},{"key":"S0022149X2000022X_ref23","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0434"},{"key":"S0022149X2000022X_ref20","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.68.652"},{"key":"S0022149X2000022X_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"S0022149X2000022X_ref6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109793"},{"key":"S0022149X2000022X_ref19","first-page":"267","article-title":"Triplex polymerase chain reaction assay for detection of major soil-transmitted helminths, Ascaris lumbricoides, Trichuris trichiura, Necator americanus, in faecal samples","volume":"45","author":"Phuphisut","year":"2014","journal-title":"The Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X2000022X_ref5a","doi-asserted-by":"publisher","DOI":"10.1080\/01621459.1993.10476353"},{"key":"S0022149X2000022X_ref23a","unstructured":"World Health Organization (2010) Working to Overcome the Global Impact of Neglected Tropical Diseases. 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