{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T06:13:01Z","timestamp":1793945581960,"version":"4.8.0"},"reference-count":62,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"unspecified","delay-in-days":124,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the Laboratory of Parasites of Fishes, Crustaceans and Mollusks (CEPAVE), we undertook a parasitological study on three species of fish from the Espinal and Esteros del Iber\u00e1 ecoregions of Argentina. Clinostomid metacercariae were found parasitizing<jats:italic>Characidium rachovii<\/jats:italic>,<jats:italic>Crenicichla vittata<\/jats:italic>and<jats:italic>Gymnogeophagus balzanii<\/jats:italic>. In this study, we analysed the damage that these parasites inflict on their hosts through the evaluation of histological sections. In addition,<jats:italic>Clinostomum<\/jats:italic>metacercariae were identified using morphological characters and DNA barcoding. In the pathological analysis, we observed that muscle tissue was the most affected. The inflammatory response showed vascular congestion areas and infiltration of numerous inflammatory cells, mainly lymphocytes. The molecular and morphological approach supports the presence of three new lineages of clinostomid metacercariae in Argentina. This could lead to the discovery of a high number of lineages or species of<jats:italic>Clinostomum<\/jats:italic>from South America.<\/jats:p>","DOI":"10.1017\/s0022149x20000292","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T09:52:55Z","timestamp":1588585975000},"source":"Crossref","is-referenced-by-count":17,"title":["Pathology associated with three new<i>Clinostomum<\/i>metacercariae from Argentina with morphological and DNA barcode identification"],"prefix":"10.1017","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-333X","authenticated-orcid":false,"given":"M.M.","family":"Montes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7318-7863","authenticated-orcid":false,"given":"S.E.","family":"Plaul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5346-1981","authenticated-orcid":false,"given":"Y.","family":"Croci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Waldbillig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3995-5122","authenticated-orcid":false,"given":"W.","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3698-5131","authenticated-orcid":false,"given":"E.","family":"Topa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5375-9155","authenticated-orcid":false,"given":"S.R.","family":"Martorelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"S0022149X20000292_ref61","first-page":"942","article-title":"New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes)","volume":"15","author":"Van Steenkiste","year":"2015","journal-title":"Molecular Biology Evolution"},{"key":"S0022149X20000292_ref60","first-page":"188","volume-title":"Peces de agua dulce del Uruguay","author":"Teixeira de Mello","year":"2011"},{"key":"S0022149X20000292_ref59","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0022149X20000292_ref58","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura","year":"1993","journal-title":"Molecular Biology Evolution"},{"key":"S0022149X20000292_ref57","doi-asserted-by":"publisher","DOI":"10.1139\/f69-074"},{"key":"S0022149X20000292_ref56","doi-asserted-by":"publisher","DOI":"10.5935\/1981-2965.20140007"},{"key":"S0022149X20000292_ref55","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2013.06.018"},{"key":"S0022149X20000292_ref54","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-5905-8"},{"key":"S0022149X20000292_ref53","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3457-5"},{"key":"S0022149X20000292_ref52","doi-asserted-by":"publisher","DOI":"10.1214\/aos\/1176344136"},{"key":"S0022149X20000292_ref49","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-016-9686-0"},{"key":"S0022149X20000292_ref19","doi-asserted-by":"publisher","DOI":"10.15517\/rbt.v64i2.19602"},{"key":"S0022149X20000292_ref51","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"S0022149X20000292_ref47","doi-asserted-by":"crossref","first-page":"S175","DOI":"10.4049\/jimmunol.178.Supp.94.24","article-title":"Fish lymphocytes regulate the inflammatory response via the production of chemokines","volume":"178","author":"Roca","year":"2007","journal-title":"Journal of Immunology"},{"key":"S0022149X20000292_ref45","first-page":"97","article-title":"Euclinostomum heterostomum (Rudolphi, 1809) metacercarial infection in three osphronemid fish species","volume":"10","author":"Purivirojkul","year":"2013","journal-title":"Walailak Journal of Science and Technology"},{"key":"S0022149X20000292_ref6","doi-asserted-by":"publisher","DOI":"10.1645\/17-183"},{"key":"S0022149X20000292_ref18","first-page":"181","article-title":"Human laryngitis caused by Clinostomum complanatum","volume":"76","author":"Hara","year":"2014","journal-title":"Medical Science"},{"key":"S0022149X20000292_ref16","first-page":"263","author":"Ferguson","year":"1989","journal-title":"Systemic pathology of fish a text an atlas of comparative tissue reponses in diseases of teleosts"},{"key":"S0022149X20000292_ref13","volume-title":"Peces del Iber\u00e1. 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