{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T21:53:42Z","timestamp":1747173222556,"version":"3.40.5"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T00:00:00Z","timestamp":1588896000000},"content-version":"unspecified","delay-in-days":128,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult forms of members of the Callodistomidae always parasitize the gallbladder of freshwater fishes and occur in Africa and America. This study provides a description of a new South American species belonging in<jats:italic>Prosthenhystera<\/jats:italic>from the gallbladder of a characid fish (<jats:italic>Bryconamericus ikaa<\/jats:italic>), and ribosomal gene sequences (28S rDNA and ITS1-5.8S-ITS2) are used to demonstrate molecular differences between the new species and congeners as well as explore interrelationships among congeners. Additionally, the first cytological analysis is conducted for a member of the family to determine chromosome number and arrangement.<jats:italic>Prosthenhystera gattii<\/jats:italic>n. sp. most closely resembles<jats:italic>Prosthenhystera caballeroi<\/jats:italic>in morphology, but the vitellarium is more extensive reaching anterior to the caecal bifurcation in the new species and the uterus is confined to the hindbody in<jats:italic>P. gattii<\/jats:italic>n. sp., whereas it extends to the level of the pharynx in<jats:italic>P. caballeroi<\/jats:italic>. Also, the testes, cirrus sac, seminal receptacle and the ratio of body length to width are larger in<jats:italic>P. gattii<\/jats:italic>n. sp. Independent Bayesian inference analyses of 28S rDNA and ITS1-5.8S-ITS2 sequence fragments produced phylograms that showed<jats:italic>P. gattii<\/jats:italic>n. sp. is more similar to<jats:italic>Prosthenhystera obesa<\/jats:italic>+<jats:italic>Prosthenhystera oonastica<\/jats:italic>than<jats:italic>P<\/jats:italic>.<jats:italic>caballeroi +<\/jats:italic>two unidentified species of<jats:italic>Prosthenhystera<\/jats:italic>, but with poor posterior probability support for the node in the ITS1-5.8S-ITS2-based phylogram. Further, the genetic distance between<jats:italic>P. oonastica<\/jats:italic>and<jats:italic>P. gattii<\/jats:italic>n. sp. are the largest among<jats:italic>Prosthenhystera<\/jats:italic>spp. Cytological analysis revealed ten metacentric chromosomes, which is fewer than the 12\u201318 chromosomes present in species from the closely related Gorgoderidae.<\/jats:p>","DOI":"10.1017\/s0022149x20000322","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T08:02:09Z","timestamp":1588924929000},"source":"Crossref","is-referenced-by-count":6,"title":["<i>Prosthenhystera gattii<\/i>n. sp. (Digenea: Callodistomidae), a gallbladder parasite of<i>Bryconamericus ikaa<\/i>from the lower Iguaz\u00fa River, described based on combined molecular and morphological evidence"],"prefix":"10.1017","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-333X","authenticated-orcid":false,"given":"M.M.","family":"Montes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Barneche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Croci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Rodriguez Gil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.S.","family":"Curran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.R.","family":"Casciotta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.R.","family":"Martorelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"S0022149X20000322_ref28","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0022149X20000322_ref26","doi-asserted-by":"crossref","first-page":"113","DOI":"10.24039\/rnh201591787","article-title":"Chromosomes and cytogenetics of helminths (Turbellaria, Trematoda, Cestoda, Nematoda and acanthocephalan)","volume":"9","author":"Sofi","year":"2015","journal-title":"Neotropical Helminthology"},{"key":"S0022149X20000322_ref29","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-014-9531-2"},{"key":"S0022149X20000322_ref25","doi-asserted-by":"publisher","DOI":"10.1214\/aos\/1176344136"},{"key":"S0022149X20000322_ref30","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025546001611"},{"key":"S0022149X20000322_ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6409.2011.00472.x"},{"key":"S0022149X20000322_ref12","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-14-82"},{"key":"S0022149X20000322_ref22","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/sys029"},{"key":"S0022149X20000322_ref6","unstructured":"Drummond, AJ , Ashton, B , Buxton, S , Cheung, M , Cooper, A , Duran, C and Wilson, A (2016) Geneious v5.0.4. 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