{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T03:35:22Z","timestamp":1784259322092,"version":"3.55.0"},"reference-count":76,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"unspecified","delay-in-days":152,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Echinococcus granulosus<\/jats:italic>is the causative agent of cystic echinococcosis, which has serious impacts on human and\/or animal health, resulting in significant economic losses.<jats:italic>Echinococcus granulosus<\/jats:italic>comprises a number of intra-specific variants or strains at the genetic level. In Saudi Arabia, few studies were performed on genetic variations in<jats:italic>Echinococcus<\/jats:italic>species. Therefore, the present study aimed to investigate the phenotypic and genetic characterization of hydatid cysts harboured by sheep and camels in Al-Madinah Al-Munawarah. Samples of hydatid cysts were collected from local sheep (<jats:italic>n<\/jats:italic>= 25) and camels (<jats:italic>n<\/jats:italic>= 8). The morphological criteria of protoscoleces were investigated. To investigate the molecular characterization, random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR), single-stranded conformation polymorphism (SSCP) were carried out. DNA was extracted from individual fertile cysts and subjected to RAPD-PCR analysis (using five arbitrary primers) and PCR amplification of cytochrome<jats:italic>c<\/jats:italic>oxidase I (<jats:italic>cox1<\/jats:italic>) and 12S ribosomal ribonucleic acid (12S rRNA) genes. The PCR products were subjected to SSCP analysis for genetic discrimination in<jats:italic>E. granulosus<\/jats:italic>isolates. In addition, partially sequencing of the mitochondrial DNA<jats:italic>cox1<\/jats:italic>genes was achieved for assessing the phylogenetic positions of collected isolates using some global published sequence data of<jats:italic>cox1<\/jats:italic>genes. The rostellar hooks of camel and local sheep isolates show remarkable variability in their dimensions. Five distinct SSCP patterns were identified in the 12S rRNA gene, showing intraspecific variations in<jats:italic>E. granulosus<\/jats:italic>of camels and local sheep. Sequencing of (<jats:italic>cox1<\/jats:italic>) genes of both local sheep and camels exhibit high similarity with those of the same gene (<jats:italic>E. granulosus sensu stricto<\/jats:italic>) published in NCBI BLAST.<\/jats:p>","DOI":"10.1017\/s0022149x20000395","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T13:33:22Z","timestamp":1591018402000},"source":"Crossref","is-referenced-by-count":9,"title":["Molecular characterization of<i>Echinococcus granulosus<\/i>in livestock of Al-Madinah (Saudi Arabia)"],"prefix":"10.1017","volume":"94","author":[{"given":"N.M.","family":"AL-Mutairi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5072-1063","authenticated-orcid":false,"given":"H.A.","family":"Taha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6296-0937","authenticated-orcid":false,"given":"A.H.","family":"Nigm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,6,1]]},"reference":[{"key":"S0022149X20000395_ref76","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182098002856"},{"key":"S0022149X20000395_ref73","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(99)90043-3"},{"key":"S0022149X20000395_ref71","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90065-2"},{"key":"S0022149X20000395_ref68","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2016.04.005"},{"key":"S0022149X20000395_ref66","first-page":"1031","article-title":"Hydatidosis 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