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Rumen flukes were obtained from cattle slaughtered in the states of Tabasco and Chiapas in Mexico. Eggs were collected using a 37-\u03bcm sieve and quantified. Then, an<jats:italic>in vitro<\/jats:italic>incubation study was performed: 100 eggs were placed into the wells of polystyrene microtiter plates. Anthelmintic products were tested on the eggs at concentrations ranging from 0.0015 to 3.0 mg\/ml for rafoxanide, 0.0025 to 10.20 mg\/ml for nitroxinil and 0.0015 to 3 mg\/ml for closantel to determine the median lethal dose (LD<jats:sub>50<\/jats:sub>) and maximum lethal dose (LD<jats:sub>99<\/jats:sub>). A control group (water) was included in each plate. Three different species of rumen flukes (<jats:italic>Calicophoron brothriophoron<\/jats:italic>,<jats:italic>Calicophoron clavula<\/jats:italic>and<jats:italic>Paramphistomum cervi<\/jats:italic>) belonging to five isolates were identified. Nitroxinil had the highest efficacy against rumen fluke eggs, with an LD<jats:sub>50<\/jats:sub>of 0.11 to 65 \u03bcg\/ml, whereas rafoxanide showed the lowest efficacy with an LD<jats:sub>50<\/jats:sub>ranging from 500 to 1713 \u03bcg\/ml. Closantel showed high variability in the LD<jats:sub>50<\/jats:sub>among the different analysed isolates (17 to 122 \u03bcg\/ml). The evaluated flukicidal drugs presented differential efficacy against the development of rumen fluke eggs. The efficacy of the drugs will vary depending on the geographical area of origin of the animals.<\/jats:p>","DOI":"10.1017\/s0022149x20000607","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T08:27:22Z","timestamp":1597048042000},"source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of anthelmintic drugs against egg development of rumen flukes recovered from cattle raised in the humid tropics of Mexico"],"prefix":"10.1017","volume":"94","author":[{"given":"R.","family":"Gonz\u00e1lez-Gardu\u00f1o","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.O.","family":"Ortiz-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Alegr\u00eda-Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7482-6663","authenticated-orcid":false,"given":"O.M.","family":"Torres-Chable","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.A.","family":"Cruz-Tamayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.V.","family":"Zaragoza-Vera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"S0022149X20000607_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2018.12.010"},{"key":"S0022149X20000607_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.040"},{"key":"S0022149X20000607_ref35","doi-asserted-by":"publisher","DOI":"10.4102\/jsava.v70i2.756"},{"key":"S0022149X20000607_ref34","unstructured":"SAS (2017) SAS\/STAT user's guide (S. 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