{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:38:31Z","timestamp":1780101511938,"version":"3.54.0"},"reference-count":54,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"unspecified","delay-in-days":75,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Polycystic echinococcosis (PE) is a zoonosis endemic in the Neotropical region of the Americas. It is caused by the larval stage of the cestode <jats:italic>Echinococcus vogeli<\/jats:italic>, which develops as harmful cysts that slowly grow in the liver, lungs and other organs of humans and other host species. Human PE diagnosis is usually based on clinical and epidemiological aspects and imaging techniques, often requiring confirmation by immunological assays. The currently available serological tests for detecting antibodies against <jats:italic>Echinococcus<\/jats:italic> spp. are mostly based on complex, variable and poorly characterized mixtures of native parasite antigens, which impairs specificity and\/or sensitivity. In this scenario, the evaluation of well-characterized alternative antigens is urgently needed for the improvement of PE diagnosis. Here, two subunits (AgB8\/1 and AgB8\/2) of the major secretory antigen from <jats:italic>Echinococcus granulosus<\/jats:italic> (antigen B (AgB)), of diagnostic value for cystic echinococcosis, were validated for PE diagnosis. These antigens, produced as pure recombinant proteins (rAgB8\/1 and rAgB8\/2) in <jats:italic>Escherichia coli<\/jats:italic>, allowed detecting specific immunoglobulin G antibodies in sera from PE patients in an enzyme-linked immunosorbent assay, with sensitivities of 83.72% and 81.40%, respectively, and specificities of 83.12% and 80.09%, respectively. The use of recombinant proteins overcomes difficulties to obtain parasite material and reduced non-specific reactions and costs. Our results demonstrated reproducibility and accuracy high enough to be considered valid according to the acceptance criteria for Food and Drug Administration assay validation. This qualifies rAgB8\/1 and rAgB8\/2 as potential substitutes for the currently used parasite crude or partially purified antigens.<\/jats:p>","DOI":"10.1017\/s0022149x22000086","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T09:24:45Z","timestamp":1647509085000},"source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of two heterologous recombinant antigens for the serological diagnosis of human polycystic echinococcosis"],"prefix":"10.1017","volume":"96","author":[{"given":"D.","family":"Daipert-Garcia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.G.","family":"Virginio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.B.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.G.","family":"Siqueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.B.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6420-3400","authenticated-orcid":false,"given":"R.","family":"Rodrigues-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"S0022149X22000086_ref19","doi-asserted-by":"publisher","DOI":"10.1515\/ap-2016-0019"},{"key":"S0022149X22000086_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.09.001"},{"key":"S0022149X22000086_ref10","first-page":"164","article-title":"Genetic variability of antigen B8\/1 among Echinococcus granulosus isolates from human, cattle, and sheep in Fars province, Southern Iran","volume":"6","author":"Esfedan","year":"2018","journal-title":"Reports of Biochemistry and Molecular Biology"},{"key":"S0022149X22000086_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2012.01.012"},{"key":"S0022149X22000086_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02602-3"},{"key":"S0022149X22000086_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00256-0"},{"key":"S0022149X22000086_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00316-4"},{"key":"S0022149X22000086_ref37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009648"},{"key":"S0022149X22000086_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.11.002"},{"key":"S0022149X22000086_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00069-3"},{"key":"S0022149X22000086_ref41","doi-asserted-by":"publisher","DOI":"10.5662\/wjm.v5.i4.185"},{"key":"S0022149X22000086_ref45","first-page":"243","article-title":"Hidatidose\/equinococose na amaz\u00f4nia oriental brasileira \u2013 atualiza\u00e7\u00e3o da casu\u00edstica","volume":"35","author":"Silva","year":"2002","journal-title":"Revista da Sociedade Brasileira de Medicina Tropical"},{"key":"S0022149X22000086_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90005-4"},{"key":"S0022149X22000086_ref49","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822004000700012"},{"key":"S0022149X22000086_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2015.12.006"},{"key":"S0022149X22000086_ref51","unstructured":"USDHHS, FDA, CDER. 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