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Helminthol."],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In Argentina, the family Diplostomidae is composed of eight genera:<jats:italic>Austrodiplostomum<\/jats:italic>Szidat &amp; Nani;<jats:italic>Diplostomum<\/jats:italic>von Nordmann;<jats:italic>Dolichorchis<\/jats:italic>Dubois;<jats:italic>Hysteromorpha<\/jats:italic>Lutz;<jats:italic>Neodiplostomum<\/jats:italic>Railliet;<jats:italic>Posthodiplostomum<\/jats:italic>Dubois;<jats:italic>Sphincterodiplostomum<\/jats:italic>Dubois; and<jats:italic>Tylodelphys<\/jats:italic>Diesing. During a parasitological survey of fishes from the Iguaz\u00fa National Park we detected diplostomid metacercariae in the brain of<jats:italic>Erythrinus<\/jats:italic>cf.<jats:italic>erythrinus<\/jats:italic>. Fish were caught using crab traps, transported alive to the field laboratory, cold-anaesthetized and euthanized by cervical dissection. Some metacercariae were heat-killed in water and fixed in 10% formalin and others were preserved in alcohol 96% for DNA extraction. They were sequenced for the partial segment of the 28S rDNA, internal transcribed spacer (ITS) rDNA and cytochrome c oxidase subunit I (COI) mtDNA genes. Phylogenetic reconstruction was carried out using Bayesian inference and the proportion (p) of absolute nucleotide sites (p-distance) was obtained. In the 28S rDNA tree, the metacercaria sequenced grouped as<jats:italic>Dolichorchis<\/jats:italic>sp. The COI mtDNA p-distance between the metacercariae with<jats:italic>Dolichorchis lacombeensis<\/jats:italic>was 0.01. There is a small number of ITS sequences for the Diplostomidae family deposited in the GenBank. The oral sucker, ventral sucker, holdfast organ and the distance between oral and ventral suckers are larger in the adult compared with the metacercariae. Additionally, hind-body length and width are larger in the adult due to the development of the genital complex. Further studies using an integrative approach will help confirm the affiliation of other species to the genus<jats:italic>Dolichorchis<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0022149x22000487","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T07:36:44Z","timestamp":1660808204000},"source":"Crossref","is-referenced-by-count":3,"title":["Metacercariae in the brain of<i>Erythrinus<\/i>cf.<i>erythrinus<\/i>(Characiformes: Erythrinidae) from Iguaz\u00fa National Park (Argentina): do they belong to<i>Dolichorchis lacombeensis<\/i>(Digenea, Diplostomidae)?"],"prefix":"10.1017","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-333X","authenticated-orcid":false,"given":"M.M.","family":"Montes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Croci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Santopolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Barneche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.F.","family":"Reig Cardarella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.R.","family":"Martorelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,8,18]]},"reference":[{"key":"S0022149X22000487_ref57","first-page":"141","volume-title":"The collection and preservation of animal parasites","author":"Pritchard","year":"1982"},{"key":"S0022149X22000487_ref22","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X1500005X"},{"key":"S0022149X22000487_ref75","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12358"},{"key":"S0022149X22000487_ref26","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-017-9745-1"},{"key":"S0022149X22000487_ref14","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X09990083"},{"key":"S0022149X22000487_ref1","doi-asserted-by":"publisher","DOI":"10.1645\/19-61"},{"key":"S0022149X22000487_ref55","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015696622961"},{"key":"S0022149X22000487_ref50","first-page":"20","article-title":"Systematic relationships among six species of Diplostomum Nordmann, 1832 (Digenea) based on morphological and molecular data","volume":"47(1)","author":"Niewiadomska","year":"2002","journal-title":"Acta Parasitologica"},{"key":"S0022149X22000487_ref7","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012001989"},{"key":"S0022149X22000487_ref77","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-017-5503-1"},{"key":"S0022149X22000487_ref44","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008929526011"},{"key":"S0022149X22000487_ref47","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-021-07234-4"},{"key":"S0022149X22000487_ref72","first-page":"186","volume-title":"Interrelationships of the Platyhelminthes","author":"Tkach","year":"2001"},{"key":"S0022149X22000487_ref32","first-page":"20","article-title":"Systematic relationships among six species of Diplostomum Nordmann, 1832 (Digenea) based on morphological and molecular data","volume":"47(1)","author":"Laskowski","year":"2002","journal-title":"Acta Parasitologica"},{"key":"S0022149X22000487_ref74","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-020-09928-2"},{"key":"S0022149X22000487_ref76","first-page":"1","article-title":"Molecular and morphological characterisation of the metacercariae of two species of Cardiocephaloides (Digenea: Strigeidae) infecting endemic South African klipfish (Perciformes: Clinidae)","volume":"68(1)","author":"Vermaak","year":"2021","journal-title":"Folia Parasitologica"},{"key":"S0022149X22000487_ref31","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"S0022149X22000487_ref33","first-page":"348","article-title":"Parisites of the stoan loache Barbatula conilobus (Balitoridae: Nemacheilinae) in the Dzabkhan river (Mongolia)","volume":"53","author":"Lebedeva","year":"2019","journal-title":"Parazitologiya"},{"key":"S0022149X22000487_ref51","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00049-3"},{"key":"S0022149X22000487_ref67","unstructured":"Stokes, M , Chen, F and Gunes, F (2014) An introduction to Bayesian analysis with SAS\/STAT software. pp. 1\u201325. 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