{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T21:54:11Z","timestamp":1747173251517,"version":"3.40.5"},"reference-count":17,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"unspecified","delay-in-days":275,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect <jats:italic>Trichinella<\/jats:italic> spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable <jats:italic>Trichinella spiralis<\/jats:italic> larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015\/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.<\/jats:p>","DOI":"10.1017\/s0022149x2200061x","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T10:39:15Z","timestamp":1664793555000},"source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of <i>Trichinella<\/i> larvae in meat"],"prefix":"10.1017","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7870-2713","authenticated-orcid":false,"given":"S.","family":"Vasilev","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Suvajdzic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Mitic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lj.","family":"Sabljic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Karabasil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lj.","family":"Sofronic Milosavljevic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Vasilev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"S0022149X2200061X_ref10","first-page":"136","article-title":"Occupational rhinitis due to pepsin","volume":"16","author":"Marqu\u00e8s","year":"2006","journal-title":"Journal of Investigational Allergology and Clinical Immunology"},{"key":"S0022149X2200061X_ref4","first-page":"7","article-title":"Commission implementing regulation (EU) 2015\/1375 of 10 August 2015 laying down specific rules on official controls for Trichinella in meat","volume":"212","year":"2015","journal-title":"Official Journal of the European Union L"},{"key":"S0022149X2200061X_ref3","doi-asserted-by":"publisher","DOI":"10.2298\/VETGL1306329D"},{"key":"S0022149X2200061X_ref16","doi-asserted-by":"publisher","DOI":"10.2298\/VETGL181220006V"},{"key":"S0022149X2200061X_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2019.107807"},{"key":"S0022149X2200061X_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2018.01.001"},{"key":"S0022149X2200061X_ref6","unstructured":"International Commission on Trichinellosis guidelines (2022). Available at http:\/\/trichinellosis.org\/Guidelines.html"},{"key":"S0022149X2200061X_ref9","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.12.2896"},{"key":"S0022149X2200061X_ref2","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/0091-6749(84)90513-X","article-title":"Occupational asthma due to pepsin","volume":"73","author":"Cartier","year":"1984","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"S0022149X2200061X_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2018.04.001"},{"key":"S0022149X2200061X_ref7","unstructured":"ISO\/IEC 17043:2010. General requirements for proficiency testing."},{"key":"S0022149X2200061X_ref15","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.vetpar.2013.01.031","article-title":"Trichinella detection: identification and statistical evaluation of sources of error in the magnetic stirrer method for pooled sample digestion","volume":"194","author":"Riehn","year":"2013","journal-title":"Veterinary Parasitology"},{"key":"S0022149X2200061X_ref17","doi-asserted-by":"publisher","DOI":"10.18485\/meattech.2021.62.2.7"},{"key":"S0022149X2200061X_ref12","first-page":"55354","article-title":"Magnetic stirrer method for the detection of Trichinella larvae in muscle samples","volume":"3","author":"Mayer-Scholl","year":"2017","journal-title":"Journal of Visualized Experiments"},{"key":"S0022149X2200061X_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2016.04.009"},{"key":"S0022149X2200061X_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00041"},{"key":"S0022149X2200061X_ref8","unstructured":"ISO standard 18743:2015. Microbiology of the food chain - Detection of Trichinella larvae in meat by artificial digestion method."}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X2200061X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T10:39:24Z","timestamp":1664793564000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X2200061X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":17,"alternative-id":["S0022149X2200061X"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x2200061x","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"type":"print","value":"0022-149X"},{"type":"electronic","value":"1475-2697"}],"subject":[],"published":{"date-parts":[[2022]]},"article-number":"e71"}}