{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T21:52:08Z","timestamp":1747173128431,"version":"3.40.5"},"reference-count":10,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"unspecified","delay-in-days":136,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>We investigated parasitic zoonoses caused by protozoans and helminths in urban and peri-urban rat populations (<jats:italic>Rattus norvegicus<\/jats:italic> and <jats:italic>R. rattus<\/jats:italic>) in Spanish cities. Rats were trapped and then dissected to remove adult helminths, and the contents of the large intestine were retrieved for the study of parasitic forms. The Midi Parasep\u00ae solvent free (SF) technique was used to concentrate the parasites in the intestinal contents. Some of the rats studied (n = 8) were infected by the rat lungworm, <jats:italic>Angiostongylus cantonensis<\/jats:italic>, whose first stage larvae (L1) are shed in rat faeces. After the concentration technique, L1 larvae were found in the sediment of 6 of the 8 positive rats. The two negative sediment samples were due to the presence of either only adult females or, in addition to males, only young females in the lungs of the rats. In view of our results, Midi Parasep\u00ae SF turned out to be a simple, rapid, inexpensive, and sensitive method to detect nematode larvae, such as the L1 larvae of <jats:italic>A. cantonensis<\/jats:italic> (or <jats:italic>A. costaricensis<\/jats:italic>), in natural and experimentally infected rats.<\/jats:p>","DOI":"10.1017\/s0022149x23000251","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T07:31:27Z","timestamp":1684308687000},"source":"Crossref","is-referenced-by-count":1,"title":["Application of the Midi Parasep\u00ae SF technique for the detection of L1 larvae of <i>Angiostrongylus cantonensis<\/i> in faeces of infected rats"],"prefix":"10.1017","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6712-9461","authenticated-orcid":false,"given":"M. Teresa","family":"Gal\u00e1n-Puchades","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"S\u00e1ez-Dur\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"G\u00f3mez-Sambl\u00e1s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Osuna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Bueno-Mar\u00ed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.V.","family":"Fuentes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"S0022149X23000251_r3","doi-asserted-by":"publisher","DOI":"10.3201\/eid2506.181027"},{"key":"S0022149X23000251_r10","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/BF00925940","article-title":"The first record of Angiostrongylus cantonensis\u00a0from Egypt","volume":"56","author":"Yousif","year":"1978","journal-title":"Zeitschrift fur Parasitenkunde"},{"key":"S0022149X23000251_r2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.06.023"},{"key":"S0022149X23000251_r8","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05338-z"},{"key":"S0022149X23000251_r1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/try010"},{"key":"S0022149X23000251_r4","first-page":"311","article-title":"One Health Approach to zoonotic parasites: molecular detection of intestinal protozoans in an urban population of Norway rats","volume":"10","author":"Gal\u00e1n-Puchades","year":"2021","journal-title":"Rattus norvegicus, in Barcelona, Spain. Pathogens"},{"key":"S0022149X23000251_r6","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12040567"},{"key":"S0022149X23000251_r7","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06776-3"},{"key":"S0022149X23000251_r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2012.10.008"},{"key":"S0022149X23000251_r5","doi-asserted-by":"publisher","DOI":"10.3201\/eid2812.220418"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X23000251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T05:29:05Z","timestamp":1690781345000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X23000251\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":10,"alternative-id":["S0022149X23000251"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x23000251","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"type":"print","value":"0022-149X"},{"type":"electronic","value":"1475-2697"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e43"}}