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Helminthol."],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Trichinellosis is a global food-borne disease caused by viviparous parasitic nematodes of the genus <jats:italic>Trichinella.<\/jats:italic> Due to the lack of effective, safe therapy and the documented adverse effects of traditional therapy, this study aimed to evaluate the therapeutic effect of acetazolamide-loaded silver nanoparticles (AgNPs) on murine trichinellosis. Fifty male Swiss albino mice were divided into five groups of ten mice each: Group I, normal control group; Group II, infected with <jats:italic>T. spiralis<\/jats:italic> and not treated; Group III, infected and given AgNPs; Group IV, infected and treated with acetazolamide; and Group V, infected and treated with acetazolamide-loaded AgNPs. Mice were infected orally with 250 larvae. The efficacy was assessed by counting <jats:italic>T. spiralis<\/jats:italic> adults and larvae, measuring serum total antioxidant capacity, and observing the histopathological and ultrastructural alterations. Acetazolamide-loaded AgNPs treatment exhibited the highest percentage of reduction (84.72% and 80.74%) for the intestinal adults and the muscular larvae of <jats:italic>T. spiralis-<\/jats:italic>infected animals, respectively. Furthermore, during the intestinal and muscular phases, the serum of the same group had the best free-radical scavenging capacity (antioxidant capacity), which reduced tissue damage induced by oxidative stress. Histopathologically, the normal intestinal and muscular architecture was restored in the group treated with acetazolamide-loaded AgNPs, in addition to the reduced inflammatory infiltrate that alleviated inflammation compared to infected animals. Our results confirmed the marked destruction of the ultrastructural features of <jats:italic>T. spiralis<\/jats:italic> adults and larvae. Acetazolamide-loaded AgNPs are a promising therapy against <jats:italic>T. spiralis<\/jats:italic> infection.<\/jats:p>","DOI":"10.1017\/s0022149x23000731","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T09:32:35Z","timestamp":1700127155000},"source":"Crossref","is-referenced-by-count":5,"title":["Acetazolamide loaded-silver nanoparticles: A potential treatment for murine trichinellosis"],"prefix":"10.1017","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4039-2710","authenticated-orcid":false,"given":"E.F.","family":"Abdel Hamed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.A.","family":"Taha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.M.","family":"Abdel 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