{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T07:40:09Z","timestamp":1736149209792,"version":"3.32.0"},"reference-count":21,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"unspecified","delay-in-days":371,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Gastrointestinal tract (GIT) nematode infections have a significant negative impact on the well-being and productivity of animals. While it is common for a host to be co-infected with multiple species of nematode parasites simultaneously, there is a lack of effective tools to study the composition of these complex parasite communities. We describe the application of the \u201cnemabiome\u201d amplicon sequencing to study parasitic GIT nematode communities in captive wildlife at the National Zoological Garden, South African National Biodiversity Institute. A total of 13 samples from Roan antelope (n=1), Eland (n=1), Sable antelope (n=9), Arabian oryx (n=1), and Blue duiker (n=1) were analysed for the presence of GIT nematode infections. A total of 256 operational taxonomical units (OTUs) were generated and compared to a curated database with \u226599% identity to sequences in the NCBI database. The OTUs found represented nematode species which are commonly associated with wild ruminants. These belonged to members of the genera <jats:italic>Haemonchus, Trichostrongylus<\/jats:italic>, and <jats:italic>Cooperia<\/jats:italic>, with a few OTUs classified as unidentified genera detected. These were further classified to species level, with <jats:italic>Haemonchus contortus and Trichostrongylus colubriformis<\/jats:italic> having high relative abundance across all captive ungulate species. Utilizing amplicon sequencing to examine the nemabiome holds great potential for enhancing our basic knowledge of the biology of gastrointestinal nematodes and monitoring infections. This improved understanding could guide the development of more efficacious preventive and control measures against these significant parasites, which negatively impact animal health and productivity on a global scale.<\/jats:p>","DOI":"10.1017\/s0022149x24000804","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T06:58:03Z","timestamp":1736146683000},"source":"Crossref","is-referenced-by-count":0,"title":["Short communication: Exploring gastrointestinal tract nemabiomes in captive ungulates at the SANBI National Zoological Gardens: A next-generation sequencing baseline approach"],"prefix":"10.1017","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5707-9221","authenticated-orcid":false,"given":"V.","family":"Phetla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Masenya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Chaisi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"S0022149X24000804_r5","first-page":"265","article-title":"Parasites of South African wildlife. XIV. Helminths of nyalas (Tragelaphus angasii) in the Mkuzi Game Reserve, KwaZulu-Natal","volume":"63","author":"Boomker","year":"1996","journal-title":"Onderstepoort Journal of Veterinary Research"},{"key":"S0022149X24000804_r7","first-page":"95","article-title":"Prevalence of gastrointestinal helminths in domestic ungulates from a semi-arid region of Maharashtra, India","volume":"46","author":"Chaudhari","year":"2022","journal-title":"Journal of Parasitic Diseases"},{"key":"S0022149X24000804_r9","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"S0022149X24000804_r13","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-11561-x"},{"key":"S0022149X24000804_r14","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X24000361"},{"key":"S0022149X24000804_r16","first-page":"105","article-title":"The use of ITS-2 rDNA nemabiome metabarcoding to enhance anthelmintic resistance diagnosis and surveillance of ovine gastrointestinal nematodes","volume":"14","author":"Queiroz","year":"2020","journal-title":"International Journal for Parasitology: Drugs and Drug Resistance"},{"key":"S0022149X24000804_r1","first-page":"138","article-title":"The prevalence of gastrointestinal parasites in wild and domestic animals in Radom National Park; South Darfur state, Sudan","volume":"59","author":"Abuessailla","year":"2013","journal-title":"Assiut Veterinary Medical Journal"},{"key":"S0022149X24000804_r12","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0553"},{"key":"S0022149X24000804_r2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143559"},{"key":"S0022149X24000804_r10","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05449-7"},{"key":"S0022149X24000804_r6","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"S0022149X24000804_r19","unstructured":"Sulliotti, V (2023) Protostrongylids nematodes as a tool to study the biogeography of wild mammals. The University of Bologna. PhD dissertation. Accessed 05 April 2024."},{"key":"S0022149X24000804_r17","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2584"},{"key":"S0022149X24000804_r18","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90021-P"},{"key":"S0022149X24000804_r20","first-page":"137","article-title":"Helminths and botfly larvae of wild ungulates on a game ranch in Central Province, Zambia","volume":"65","author":"Zieger","year":"1998","journal-title":"Onderspoort Journal of Veterinary Research"},{"key":"S0022149X24000804_r15","first-page":"337","article-title":"Pest or guest: a ruminant nemabiome interactome through the application of trait-based ecology","volume":"10","author":"Pittman","year":"2020","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"S0022149X24000804_r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2017.06.006"},{"key":"S0022149X24000804_r4","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-05087-5"},{"key":"S0022149X24000804_r8","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.10.2525"},{"key":"S0022149X24000804_r11","first-page":"130","article-title":"Synoptic review of Nematoda (Trichocephalida, Enoplida, Trichinellida) in captive and feral ungulates from the Americas","volume":"86","author":"Hoberg","year":"2015","journal-title":"Revista Mexicana de Biodiversidad"},{"key":"S0022149X24000804_r21","volume-title":"North-West University","author":"Mosala","year":"2017"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X24000804","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T06:58:07Z","timestamp":1736146687000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X24000804\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":21,"alternative-id":["S0022149X24000804"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x24000804","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e89"}}