{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T03:45:51Z","timestamp":1794109551564,"version":"4.8.0"},"reference-count":56,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"unspecified","delay-in-days":65,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001138","name":"Australian Biological Resources Study","doi-asserted-by":"publisher","award":["NTRGII000024"],"award-info":[{"award-number":["NTRGII000024"]}],"id":[{"id":"10.13039\/501100001138","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The techniques employed to collect and store trematodes vary between research groups, and although these differences are sometimes necessitated by distinctions in the hosts examined, they are more commonly an artefact of instruction. As a general rule, we tend to follow what we were taught rather than explore new techniques. A major reason for this is that there are few technique papers in the published literature. Inspired by a collaborative workshop at the\n                    <jats:italic>Trematodes 2024<\/jats:italic>\n                    symposium, we outline our techniques and processes for collecting adult trematodes from fishes and discuss the improvements we have made over 40 years of dissections of 20,000+ individual marine fishes. We present these techniques for two reasons: first, to encourage unified methods across the globe, with an aim to produce optimally comparable specimens across temporal periods, across geographic localities, and between research groups; and second, as a resource for inexperienced researchers. We stress the importance of understanding differences in host biology and the expected trematode fauna, which ultimately enables organised and productive dissections. We outline our dissection method for each key organ separately, discuss handling, fixation, and storage methods to generate the most uniform and comparable samples, and explore ethical considerations, issues of accurate host identification, and the importance and potential of clear record keeping.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x24000968","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T02:46:11Z","timestamp":1741315571000},"source":"Crossref","is-referenced-by-count":13,"title":["Twenty thousand fishes under the seas: Insights into the collection and storage of trematodes from the examination of 20,000 fishes in the tropical Indo west-Pacific"],"prefix":"10.1017","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5648-3684","authenticated-orcid":false,"given":"S.C.","family":"Cutmore","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7131-1875","authenticated-orcid":false,"given":"R.A.","family":"Bray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1015-4703","authenticated-orcid":false,"given":"D.C.","family":"Huston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8071-4609","authenticated-orcid":false,"given":"S.B.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-1394","authenticated-orcid":false,"given":"T.L.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8303-8714","authenticated-orcid":false,"given":"N.Q-X.","family":"Wee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2211-1468","authenticated-orcid":false,"given":"R.Q-Y.","family":"Yong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1092-9705","authenticated-orcid":false,"given":"T.H.","family":"Cribb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,3,7]]},"reference":[{"key":"S0022149X24000968_r54","first-page":"249","article-title":"Studies on the helminth fauna of Japan. Part II","volume":"5","author":"Yamaguti","year":"1934","journal-title":"Trematodes of fishes, I. Japanese Journal of Zoology"},{"key":"S0022149X24000968_r10","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2010.08.003"},{"key":"S0022149X24000968_r49","first-page":"85","volume-title":"Metazoan Parasites in the Neotropics: A Systematic and Ecological Perspective","author":"Scholz","year":"2000"},{"key":"S0022149X24000968_r4","first-page":"359","article-title":"Hallazgo de una especie nueva del g\u00e9nero Petalodistomum Johnston, 1913 (Trematoda: Gorgoderidae) en los tiburones de las Costas de Manzanillo, Colima","volume":"16","author":"Caballeroy","year":"1945","journal-title":"Anales del Instituto de Biologia Universidad Nacional Autonoma de Mexico Serie Zoologia"},{"key":"S0022149X24000968_r55","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2017.12.003"},{"key":"S0022149X24000968_r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2018.08.003"},{"key":"S0022149X24000968_r46","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2020.04.008"},{"key":"S0022149X24000968_r2","doi-asserted-by":"publisher","DOI":"10.1080\/00222938400770211"},{"key":"S0022149X24000968_r56","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013000681"},{"key":"S0022149X24000968_r53","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.09.004"},{"key":"S0022149X24000968_r9","first-page":"225","volume-title":"Taxonomy, Ecology and Evolution of Metazoan Parasites. (Livre hommage \u00e0 Louis Euzet)","author":"Curran","year":"2003"},{"key":"S0022149X24000968_r21","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X20000383"},{"key":"S0022149X24000968_r8","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006287219312"},{"key":"S0022149X24000968_r28","first-page":"93","article-title":"Two new species of Xystretrum Linton, 1910 (Trematoda; Gorgoderidae) from fishes of Queensland, Australia","volume":"41","author":"Manter","year":"1972","journal-title":"Anales del Instituto de Biolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico (Series Zoolog\u00eda)"},{"key":"S0022149X24000968_r50","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.10.002"},{"key":"S0022149X24000968_r30","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-018-9826-9"},{"key":"S0022149X24000968_r52","first-page":"36","article-title":"A new anaporrhutine trematode (Fam. Gorgoderidae) Nagmia yamagutia n. sp. from the ovary of the ray, Dasyatis uarnak, from Bharat (India)","volume":"42","author":"Tandon","year":"1969","journal-title":"Annotationes Zoologicae Japonenses"},{"key":"S0022149X24000968_r3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1987.tb05229.x"},{"key":"S0022149X24000968_r31","first-page":"79","article-title":"Plethorchis acanthus gen. et sp. n. (Trematoda: Sanguinicolidae) in mullet, Mugil cephalus L., from Queensland, Australia","volume":"42","author":"Martin","year":"1975","journal-title":"Proceedings of the Helminthological Society of Washington"},{"key":"S0022149X24000968_r18","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.3779.5.5"},{"key":"S0022149X24000968_r15","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13495"},{"key":"S0022149X24000968_r19","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.2442.1.2"},{"key":"S0022149X24000968_r11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2023.06.006"},{"key":"S0022149X24000968_r7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2021.09.002"},{"key":"S0022149X24000968_r26","first-page":"95","article-title":"Zoogonidae (Trematoda, Digenea) from fishes of Japanese waters","volume":"32","author":"Machida","year":"2006","journal-title":"Bulletin of the National Science Museum Series A (Zoology)"},{"key":"S0022149X24000968_r27","doi-asserted-by":"publisher","DOI":"10.2307\/3277609"},{"key":"S0022149X24000968_r14","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-021-10002-8"},{"key":"S0022149X24000968_r12","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2021072"},{"key":"S0022149X24000968_r23","doi-asserted-by":"publisher","DOI":"10.1016\/j.fishres.2008.10.006"},{"key":"S0022149X24000968_r17","doi-asserted-by":"publisher","DOI":"10.1007\/BF00012194"},{"key":"S0022149X24000968_r25","first-page":"157","volume-title":"Studies in Avian Biology. The Role of Collections in Ornithology: The Extended Specimen","author":"Lutz","year":"2017"},{"key":"S0022149X24000968_r33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2020.03.016"},{"key":"S0022149X24000968_r35","unstructured":"Nagaty, HF (1937) Trematodes of fishes from the Red Sea. Part 1. Studies on the family Bucephalidae Poche, 1907. Egyptian University, Faculty of Medicine, Publication No. 12."},{"key":"S0022149X24000968_r20","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-2845-6"},{"key":"S0022149X24000968_r37","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.50.183"},{"key":"S0022149X24000968_r42","first-page":"317","article-title":"Hapalorhynchus brooksi sp. n. (Trematoda: Spirorchiidae) from the Snapping turtle (Chelydra serpentina), with notes on H. gracilis and H. stunkardi","volume":"55","author":"Platt","year":"1988","journal-title":"Proceedings of the Helminthological Society of Washington"},{"key":"S0022149X24000968_r39","unstructured":"Pallas, PS (1774) Spicilegia zoologica quibas novae imprimus et obscurae animalium species inconibus, descriptionibus atque commentariis illustrantur. Fasc. 10. Berolini."},{"key":"S0022149X24000968_r5","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-010-9229-z"},{"key":"S0022149X24000968_r6","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182022000282"},{"key":"S0022149X24000968_r29","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2024.09.012"},{"key":"S0022149X24000968_r43","doi-asserted-by":"publisher","DOI":"10.2307\/3284166"},{"key":"S0022149X24000968_r47","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.734401"},{"key":"S0022149X24000968_r48","volume-title":"The Collection and Preservation of Animal Parasites","author":"Pritchard","year":"1982"},{"key":"S0022149X24000968_r1","doi-asserted-by":"publisher","DOI":"10.1080\/00288330.2021.1914689"},{"key":"S0022149X24000968_r22","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2015.04.003"},{"key":"S0022149X24000968_r36","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.1218.1.1"},{"key":"S0022149X24000968_r16","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-022-10034-8"},{"key":"S0022149X24000968_r34","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmb.2017.03.020"},{"key":"S0022149X24000968_r41","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X17000189"},{"key":"S0022149X24000968_r44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2008.03.024"},{"key":"S0022149X24000968_r24","first-page":"11","article-title":"Helminth fauna of the Dry Tortugas. II. Trematodes","volume":"4","author":"Linton","year":"1910","journal-title":"Papers from the Tortugas Laboratory of the Carnegie Institute of Washington"},{"key":"S0022149X24000968_r40","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2260.1"},{"key":"S0022149X24000968_r45","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2010.0640"},{"key":"S0022149X24000968_r38","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-017-9733-5"},{"key":"S0022149X24000968_r51","doi-asserted-by":"publisher","DOI":"10.1654\/4155"},{"key":"S0022149X24000968_r32","first-page":"e10828","article-title":"Size bias in the documentation of marine biodiversity","volume":"2024","author":"McClain","year":"2024","journal-title":"Oikos"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X24000968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T02:46:21Z","timestamp":1741315581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X24000968\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":56,"alternative-id":["S0022149X24000968"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x24000968","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e45"}}