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Helminthol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>Here, we present a comprehensive morphological and molecular phylogenetic analysis of <jats:italic>Clinostomum<\/jats:italic> sp. (Digenea: Clinostomidae) metacercariae parasitizing two freshwater fish species from Southeast Brazil: <jats:italic>Serrasalmus spilopleura<\/jats:italic> (piranha) and <jats:italic>Callichthys callichthys<\/jats:italic> (tambuat\u00e1). The morphological examination revealed distinct characteristics of metacercariae in each host. Using the cytochrome c oxidase I (COI) gene barcode region, we obtained DNA sequences that allowed for accurate phylogenetic placement. Phylogenetic analyses revealed that <jats:italic>Clinostomum<\/jats:italic> sp. HM41 (metacercariae), isolated from <jats:italic>S. spilopleura<\/jats:italic>, exhibited 86% similarity to <jats:italic>Ithyoclinostomum yamagutii<\/jats:italic>, while <jats:italic>Clinostomum<\/jats:italic> sp. HM125 (metacercariae), from <jats:italic>C. callichthys<\/jats:italic>, showed 98.7% similarity to <jats:italic>Clinostomum<\/jats:italic> sp. Cr_Ha1. The phylogenetic trees constructed through Bayesian Inference and Maximum Likelihood methods indicated high biodiversity within the <jats:italic>Clinostomum<\/jats:italic> genus and strong support for distinct lineages. These findings enhance our understanding of the diversity and ecological distribution of <jats:italic>Clinostomum<\/jats:italic> species in South American freshwater environments.<\/jats:p>","DOI":"10.1017\/s0022149x24000993","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T05:42:42Z","timestamp":1738215762000},"source":"Crossref","is-referenced-by-count":4,"title":["Morphological and molecular phylogeny of <i>Clinostomum<\/i> sp. (Digenea: Clinostomidae) metacercariae, using DNA barcode from a South American freshwater fish"],"prefix":"10.1017","volume":"99","author":[{"given":"T.","family":"Gi\u00e3o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.I.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4139-7839","authenticated-orcid":false,"given":"L.","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.J.","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"de Azevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Abdallah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"S0022149X24000993_r28","first-page":"257","article-title":"Food of central Amazonian fishes. 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