{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T05:10:09Z","timestamp":1744261809900,"version":"3.40.4"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"unspecified","delay-in-days":99,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>The East Asian region has seen the first discovery of trematodes of the genus <jats:italic>Psilotrema<\/jats:italic> in the muskrat <jats:italic>Ondatra zibethicus.<\/jats:italic> When studying the morphology of the trematodes, two morphotypes were identified among individuals of <jats:italic>Psilotrema<\/jats:italic> spp., the differences between which at the morphological level are equivalent to those between species. The affiliation of one of these morphotypes with the bird parasite <jats:italic>Psilotrema limosum<\/jats:italic> was established based on partial sequences of genes coding for 28S ribosomal RNA, <jats:italic>nad1<\/jats:italic>, and <jats:italic>cox1<\/jats:italic> mitochondrial DNA. Based on the analysis of these three markers, we classified individuals with this morphotype as <jats:italic>P. limosum<\/jats:italic>, despite some morphological features of worms infecting birds. At the same time, the worms with second morphotype had no interspecies differences based on the first two markers, while the <jats:italic>cox1<\/jats:italic> gene confirmed the separation of these samples on a species level. These trematodes were described as a new species, <jats:italic>Psilotrema elegans<\/jats:italic> n. sp., based on both morphological and molecular data.<\/jats:p>","DOI":"10.1017\/s0022149x25000318","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T04:34:31Z","timestamp":1744259671000},"source":"Crossref","is-referenced-by-count":0,"title":["First detection of <i>Psilotrema limosum<\/i> in muskrat in East Asia and a description of <i>Psilotrema elegans<\/i> n. sp."],"prefix":"10.1017","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7706-3771","authenticated-orcid":false,"given":"K.A.","family":"Kalinina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu.V.","family":"Tatonova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.V.","family":"Besprozvannykh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.Yu.","family":"Shchelkanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"key":"S0022149X25000318_r22","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"S0022149X25000318_r23","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00163-0"},{"key":"S0022149X25000318_r16","doi-asserted-by":"publisher","DOI":"10.3390\/ani11082444"},{"key":"S0022149X25000318_r10","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2022.102554"},{"key":"S0022149X25000318_r8","first-page":"17","article-title":"Les parasites internes du rat musque-Ondatra zibethica (L.) des environs de Bydgoszcz (Pologne)","volume":"2","author":"Grabda","year":"1954","journal-title":"Acta Parasitologica Polonica"},{"key":"S0022149X25000318_r12","first-page":"103","article-title":"New data on the helminths of the muskrat (Ondatra zibethicus) in Lithuania Estonian","volume":"58","author":"Mazeika","year":"2009","journal-title":"Journal of Ecology"},{"key":"S0022149X25000318_r26","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025546001611"},{"key":"S0022149X25000318_r25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2015.11.001"},{"key":"S0022149X25000318_r18","first-page":"89","article-title":"Contributions to the knowledge of the internal parasites of muskrat (Ondatra zibethica L., 1776) living along the river Tisza","volume":"2","author":"Sey","year":"1966","journal-title":"Tiscia"},{"key":"S0022149X25000318_r9","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-1095-8"},{"key":"S0022149X25000318_r15","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg180"},{"key":"S0022149X25000318_r17","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X19001093"},{"key":"S0022149X25000318_r3","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2370.1"},{"key":"S0022149X25000318_r5","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"S0022149X25000318_r21","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182020001444"},{"key":"S0022149X25000318_r7","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2109"},{"key":"S0022149X25000318_r19","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1007\/s00436-022-07456-0","article-title":"Assessing the population structure of trematode Metagonimus suifunensis using three mitochondrial markers","volume":"121","author":"Shumenko","year":"2022","journal-title":"Parasitology Research"},{"key":"S0022149X25000318_r27","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2144\/00291bm09","article-title":"Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT)","volume":"29","author":"Truett","year":"2000","journal-title":"Biotechniques"},{"key":"S0022149X25000318_r24","first-page":"186","volume-title":"Interrelationships of Platyhelminthes","author":"Tkach","year":"2001"},{"key":"S0022149X25000318_r20","unstructured":"Skryabin, KI (1947) Trematodes of Animals and Humans, vol. 1 Fundamentals of Trematodology: Monograph. Publishing House of the USSR Academy of Sciences (in Russian)."},{"key":"S0022149X25000318_r29","first-page":"1291","article-title":"Characterization of the complete mitochondrial genome of Notocotylus sp. (Trematoda, Notocotylidae) and its phylogenetic implications","volume":"4","author":"Xu","year":"2020","journal-title":"Parasitology Research"},{"key":"S0022149X25000318_r11","first-page":"99","article-title":"The trematode fauna of rodents and insectivora mammalia in Hungary. Part 3. The occurrence of Psilotrema simillimum and Psilotrema spiculigerum equals Psilotrema marki new synonym in rodents","volume":"7","author":"Matskasi","year":"1974","journal-title":"Parasitologia Hungarica"},{"key":"S0022149X25000318_r14","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182097002217"},{"key":"S0022149X25000318_r4","doi-asserted-by":"publisher","DOI":"10.2478\/s11687-013-0131-5"},{"key":"S0022149X25000318_r1","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1645\/21-25","article-title":"Description and phylogenetic affinities of a new species of Neopsilotrema (Digenea: Psilostomidae) from lesser scaup, aythya affinis (anseriformes: anatidae)","volume":"107","author":"Achatz","year":"2021","journal-title":"Journal of Parasitology"},{"key":"S0022149X25000318_r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2010.08.003"},{"key":"S0022149X25000318_r13","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X19000828"},{"key":"S0022149X25000318_r2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.09.005"},{"key":"S0022149X25000318_r28","first-page":"6","article-title":"Psilotrema zibethica n. sp. from Ondatra zibethica","volume":"56","author":"Varenov","year":"1965","journal-title":"Scientific Notes of the Gorky State Pedagogical Institute"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X25000318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T04:34:33Z","timestamp":1744259673000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X25000318\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":29,"alternative-id":["S0022149X25000318"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x25000318","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e50"}}