{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:51:36Z","timestamp":1793386296871,"version":"4.5.0"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"unspecified","delay-in-days":113,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p><jats:italic>Ochetosoma heterocoelium<\/jats:italic> (Travassos, 1921) was collected from the mouth and oesophagus of a pit viper, <jats:italic>Bothrops moojeni<\/jats:italic> Hoge, 1966 in S\u00e3o Sebasti\u00e3o do Para\u00edso Farm (21\u00b051\u201948.26\" S, 48\u00b026\u201956.78\" W), municipality of Boa Esperan\u00e7a do Sul, S\u00e3o Paulo State, Brazil. In this study, we provide the first molecular characterisation of this digenean using 28S rDNA and COI sequences, and its phylogenetic position within the Plagiorchiida is assessed. Furthermore, new morphological features are added to the diagnosis of the species, and scanning electron microscopy photomicrographs are presented. Sequences of the 28S rRNA gene of <jats:italic>O. heterocoelium<\/jats:italic> were successfully obtained and aligned with 35 digenean species belonging to Plagiorchiida. Only three congeners \u2013 <jats:italic>O. aniarum<\/jats:italic> (Leidy, 1890), <jats:italic>O elongatum<\/jats:italic> (Seo <jats:italic>et al.<\/jats:italic> 2024), and <jats:italic>O. kansense<\/jats:italic> (Crow, 1913) \u2013 have been sequenced for this molecular marker. The newly sequenced individuals of <jats:italic>O. heterocoelium<\/jats:italic> are 98.7% and 99.4% similar to <jats:italic>O. aniarum<\/jats:italic> and <jats:italic>O. kansensis<\/jats:italic>, respectively, and <jats:italic>Ochetosoma<\/jats:italic> is not recovered as a monophyletic group. <jats:italic>Dasymetra nicolli<\/jats:italic> Holl and Allison, 1935 and <jats:italic>Lechriorchis tygarti<\/jats:italic> Talbot, 1933 are nested with <jats:italic>Ochetosoma.<\/jats:italic><\/jats:p>","DOI":"10.1017\/s0022149x25000343","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T07:20:28Z","timestamp":1745479228000},"source":"Crossref","is-referenced-by-count":1,"title":["First integrative assessment of <i>Ochetosoma heterocoelium<\/i> (Travassos, 1921) (Digenea: Ochetosomatidae) parasite of <i>Bothrops moojeni<\/i> Hoge, 1966 (Reptilia: Viperidae) from Brazil"],"prefix":"10.1017","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1946-4409","authenticated-orcid":false,"given":"E.","family":"Emmerich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Alcantara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"\u00dangari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"O\u2019Dwyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"P\u00e9rez-Ponce de Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"S0022149X25000343_r7","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-113"},{"key":"S0022149X25000343_r13","doi-asserted-by":"publisher","DOI":"10.22201\/ib.20078706e.2009.003.156"},{"key":"S0022149X25000343_r3","first-page":"11","article-title":"Ochetosoma heterocoelium (Travassos, 1921) (Trematoda: Digenea: Ochetosomatidae) in a new host","volume":"52","author":"Corr\u00eaa","year":"1990","journal-title":"Mem\u00f3rias do Instituto Butantan"},{"key":"S0022149X25000343_r12","first-page":"67","article-title":"Discuss\u00e3o em t\u00f4rno dos g\u00eaneros Ochetosoma Braun, 1901, e Renifer Pratt, 1902 (Trematoda)","volume":"18","author":"Le\u00e3o","year":"1945","journal-title":"Mem\u00f3rias do Instituto Butantan"},{"key":"S0022149X25000343_r14","doi-asserted-by":"publisher","DOI":"10.1645\/22-87"},{"key":"S0022149X25000343_r32","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0022149X25000343_r11","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"S0022149X25000343_r6","first-page":"159","article-title":"Ochetosomatid digeneans from Illinois snakes","volume":"82","author":"Dyer","year":"1989","journal-title":"Transactions of the Illinois State Academy of Science"},{"key":"S0022149X25000343_r37","first-page":"59","article-title":"Contribui\u00e7\u00f5es para o conhecimento da fauna helmintolojica brasileira. XII: sobre as especies brazileiras da sub-familia Brachycoelinae","volume":"5","author":"Travassos","year":"1921","journal-title":"Archivos da Escola Superior de Agricultura e Medicina Veterinaria, Nictheroy"},{"key":"S0022149X25000343_r21","doi-asserted-by":"publisher","DOI":"10.22201\/ib.20078706e.2012.2.949"},{"key":"S0022149X25000343_r18","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182097002217"},{"key":"S0022149X25000343_r2","doi-asserted-by":"publisher","DOI":"10.2307\/3222133"},{"key":"S0022149X25000343_r34","first-page":"411","volume-title":"Keys to the Trematoda","volume":"3","author":"Tkach","year":"2008"},{"key":"S0022149X25000343_r20","doi-asserted-by":"publisher","DOI":"10.1515\/ap-2018-0062"},{"key":"S0022149X25000343_r26","doi-asserted-by":"publisher","DOI":"10.1016\/j.ode.2005.12.005"},{"key":"S0022149X25000343_r4","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2641.1"},{"key":"S0022149X25000343_r5","first-page":"191","article-title":"\u00c9tude de quelques tr\u00e9matodes Nord-Americains (avec note sur la position systematique de Parorchis Nicoll 1907) suivie d\u2019une revision des genres Galactosomum Looss 1899 et Ochetosoma Braun 1901","volume":"82","author":"Dubois","year":"1959","journal-title":"Bulletin de la Soci\u00e9t\u00e9 des Sciences Naturelles de Neuch\u00e2tel"},{"key":"S0022149X25000343_r10","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts199"},{"key":"S0022149X25000343_r28","doi-asserted-by":"publisher","DOI":"10.3354\/dao02365"},{"key":"S0022149X25000343_r22","doi-asserted-by":"publisher","DOI":"10.1654\/4548.1"},{"key":"S0022149X25000343_r17","first-page":"1","volume-title":"Gateway Computing Environments Workshop (GCE)","author":"Miller","year":"2010"},{"key":"S0022149X25000343_r27","first-page":"1","volume-title":"Atlas zu der Reise im n\u00f6rdlichen Afrika","author":"R\u00fcppell","year":"1828"},{"key":"S0022149X25000343_r29","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2024.102947"},{"key":"S0022149X25000343_r38","first-page":"807","article-title":"Range extension and first state record of the snake parasite Renifer heterocoelium (Trematoda: Digenea) in southern Brazil","volume":"16","author":"Vicente-Ferreira","year":"2023","journal-title":"Herpetology Notes"},{"key":"S0022149X25000343_r30","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-79301999000100007"},{"key":"S0022149X25000343_r35","first-page":"267","article-title":"On the phylogenetic relationships of some members of Macroderoididae and Ochetosomatidae (Digenea, Plagiorchioidea)","volume":"46","author":"Tkach","year":"2001","journal-title":"Acta Parasitologica"},{"key":"S0022149X25000343_r1","doi-asserted-by":"publisher","DOI":"10.37828\/em.2022.54.8"},{"key":"S0022149X25000343_r25","unstructured":"Rambaut, A (2009) Molecular evolution, phylogenetics and epidemiology: Fig-Tree. 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