{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T04:05:22Z","timestamp":1746245122776,"version":"3.40.4"},"reference-count":46,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"unspecified","delay-in-days":121,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>In Japan, three trematode species of the genus <jats:italic>Azygia<\/jats:italic> have been recorded: <jats:italic>A. gotoi<\/jats:italic>, <jats:italic>A. perryii<\/jats:italic>, and <jats:italic>A. rhinogobii.<\/jats:italic> Here, we report the first detection of <jats:italic>A. hwangtsiyui<\/jats:italic> in Japan, identified from the introduced snakehead <jats:italic>Channa argus.<\/jats:italic> This trematode was previously known only from the snakeheads in mainland China. Between 2015 and 2024, we sampled snakeheads and snails in Japan, collecting adult trematodes and rediae. Adult trematodes were identified morphologically as <jats:italic>A. hwangtsiyui<\/jats:italic>, a determination corroborated by molecular analyses of the COI and 28S rDNA regions. Rediae extracted from the snail <jats:italic>Sinotaia quadrata histrica<\/jats:italic> were also identified molecularly as <jats:italic>A. hwangtsiyui<\/jats:italic>, indicating the snail acts as an intermediate host. Juvenile trematodes from the short-spined Japanese trident goby <jats:italic>Tridentiger brevispinis<\/jats:italic> were also morphologically identified as <jats:italic>A. hwangtsiyui<\/jats:italic>, indicating that this goby acts as a paratenic host. Given the snakehead\u2019s introduction to Japan between 1923 and 1924, the absence of records of later introductions, and the presence of both intermediate and paratenic hosts in Japan at that time, <jats:italic>A. hwangtsiyui<\/jats:italic> was likely introduced concurrently with the snakehead. As the detection of three COI haplotypes suggests multiple introductions, subsequent introductions via the aquarium trade or other pathways involving the transport of freshwater fish and mollusks from Asia to Japan are also plausible.<\/jats:p>","DOI":"10.1017\/s0022149x25000379","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T11:51:10Z","timestamp":1746186670000},"source":"Crossref","is-referenced-by-count":0,"title":["Introduction of <i>Azygia hwangtsiyui<\/i> (Trematoda: Azygidae) to Japan with its life cycle information"],"prefix":"10.1017","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6955-5694","authenticated-orcid":false,"given":"T.","family":"Waki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nitta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Ansai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Kitazawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Hagiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sekine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Takano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,5,2]]},"reference":[{"key":"S0022149X25000379_r14","volume-title":"The Process of Introduced New Alien Fish Species","author":"Maruyama","year":"1987"},{"key":"S0022149X25000379_r5","first-page":"269","article-title":"On a new species of the trematode genus Azygia","volume":"30","author":"Fujita","year":"1918","journal-title":"Doutsugaku Zasshi"},{"key":"S0022149X25000379_r17","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2024.102924"},{"key":"S0022149X25000379_r32","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010086329619"},{"key":"S0022149X25000379_r7","first-page":"95","article-title":"BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symposium Series"},{"key":"S0022149X25000379_r21","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2017.01.015"},{"key":"S0022149X25000379_r24","doi-asserted-by":"publisher","DOI":"10.1006\/mpev.2000.0802"},{"key":"S0022149X25000379_r41","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2005.12.017"},{"key":"S0022149X25000379_r19","first-page":"016","article-title":"Rabdochona angusticauda sp. n. 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