{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:45:45Z","timestamp":1785433545951,"version":"3.56.0"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"unspecified","delay-in-days":324,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Trichinella spiralis<\/jats:italic>\n                    (\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    ) is a zoonotic parasitic worm that has a significant impact on both public health and the livestock industry. It is widely used in experimental studies because of several unique features of its life cycle that can be completed in a single host, including distinct intestinal and muscular phases of infection, and it can be easily maintained in laboratory animals. These characteristics make\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    a valuable model for evaluating the efficacy of new drugs and vaccines against parasitic infections. The current work aims to evaluate the procedures used for\n                    <jats:italic>Trichinella<\/jats:italic>\n                    larval counting in experimental studies by comparing the magnetic stirring digestion method (MSDM) with individual muscle digestion and compression techniques under standardised, controlled conditions to assess their effectiveness and applicability. Fifteen male Swiss albino mice were orally infected with\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    and sacrificed on day 35 post-infection. They were divided into three groups: group 1\u2014MSDM for total larval count; group 2\u2014individual digestion of masseter, diaphragm, gastrocnemius, and tongue muscles; and group 3\u2014slide compression method of the same muscles. MSDM yielded the highest larval counts, with individual muscle digestion and compression methods generally producing significantly lower results, except tongue digestion, while correlation and concordance analyses identified gastrocnemius compression as an alternative despite overall poor agreement with the reference method. Therefore, MSDM is the most accurate method for larval counting in experimental studies, compared to individual muscle digestion or compression methods.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x2510093x","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:34:16Z","timestamp":1763728456000},"source":"Crossref","is-referenced-by-count":0,"title":["Towards a standardised methodology of\n                    <i>Trichinella<\/i>\n                    larval counting for research purposes"],"prefix":"10.1017","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7782-0774","authenticated-orcid":false,"given":"D.S.","family":"Ashour","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016jp5b92","id-type":"ROR","asserted-by":"publisher"}],"name":"Tanta University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.M.","family":"Elgazzar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016jp5b92","id-type":"ROR","asserted-by":"publisher"}],"name":"Tanta University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.A.","family":"Othman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016jp5b92","id-type":"ROR","asserted-by":"publisher"}],"name":"Tanta University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.S.","family":"Zoghroban","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016jp5b92","id-type":"ROR","asserted-by":"publisher"}],"name":"Tanta University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"S0022149X2510093X_r34","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-020-0741-0"},{"key":"S0022149X2510093X_r7","first-page":"593","volume-title":"Trichinella and Trichinosis","author":"Blair","year":"1983"},{"key":"S0022149X2510093X_r17","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00354-X"},{"key":"S0022149X2510093X_r2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2022.106409"},{"key":"S0022149X2510093X_r14","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00059"},{"key":"S0022149X2510093X_r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2018.10.012"},{"key":"S0022149X2510093X_r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2021.102449"},{"key":"S0022149X2510093X_r24","first-page":"199","article-title":"Comparison of different techniques and stains for direct diagnosis of Trichinella spiralis","volume":"1","author":"Mostafa","year":"2020","journal-title":"Microbes and Infectious Diseases"},{"key":"S0022149X2510093X_r27","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2015.11.001"},{"key":"S0022149X2510093X_r22","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X13000424"},{"key":"S0022149X2510093X_r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2021.e00129"},{"key":"S0022149X2510093X_r28","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.07.002"},{"key":"S0022149X2510093X_r30","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.01.031"},{"key":"S0022149X2510093X_r16","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2018.04.001"},{"key":"S0022149X2510093X_r18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.05.036"},{"key":"S0022149X2510093X_r19","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2017.07.004"},{"key":"S0022149X2510093X_r12","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.6.1043"},{"key":"S0022149X2510093X_r15","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.10.063"},{"key":"S0022149X2510093X_r1","first-page":"671","article-title":"Parasites transmitted to human by ingestion of different types of meat, El-Minia city, El-Minia governorate","volume":"45","author":"Abdel-Hafeez","year":"2015","journal-title":"Journal of the Egyptian Society of Parasitology"},{"key":"S0022149X2510093X_r31","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.12.012"},{"key":"S0022149X2510093X_r20","doi-asserted-by":"publisher","DOI":"10.1177\/104063870301500217"},{"key":"S0022149X2510093X_r33","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761997000500004"},{"key":"S0022149X2510093X_r9","doi-asserted-by":"publisher","DOI":"10.2307\/3281709"},{"key":"S0022149X2510093X_r8","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics17081069"},{"key":"S0022149X2510093X_r4","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftac040"},{"key":"S0022149X2510093X_r21","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3267-1"},{"key":"S0022149X2510093X_r32","doi-asserted-by":"publisher","DOI":"10.3390\/foods11040525"},{"key":"S0022149X2510093X_r10","unstructured":"Dupouy-Camet, J and Murrell, KD (eds) (2007) FAO\/WHO\/OIE Guidelines for the Surveillance, Management, Prevention and Control of Trichinellosis. Paris: World Organisation for Animal Health (OIE)."},{"key":"S0022149X2510093X_r29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.05.010"},{"key":"S0022149X2510093X_r11","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X21000481"},{"key":"S0022149X2510093X_r23","first-page":"55354","article-title":"Magnetic stirrer method for the detection of Trichinella larvae in muscle samples","volume":"3","author":"Mayer-Scholl","year":"2017","journal-title":"Journal of Visualized Experiments"},{"key":"S0022149X2510093X_r26","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00041"},{"key":"S0022149X2510093X_r25","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.10.075"},{"key":"S0022149X2510093X_r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2016.08.009"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X2510093X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:34:23Z","timestamp":1763728463000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X2510093X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":34,"alternative-id":["S0022149X2510093X"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x2510093x","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e123"}}