{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:28:19Z","timestamp":1767871699512,"version":"3.49.0"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"unspecified","delay-in-days":7,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study provides the first integrative analysis of\n                    <jats:italic>Megacoelium spinicavum<\/jats:italic>\n                    Thatcher &amp; Varella, 1981 (Digenea: Haploporidae) from the Amazon sailfin catfish\n                    <jats:italic>Pterygoplichthys pardalis<\/jats:italic>\n                    Castelnau, 1855 (Siluriformes: Loricariidae) in the Peruvian Amazon. A detailed morphological description is presented, including the first scanning electron microscopy (SEM) images of tegumental structures, which revealed two distinct types of tegumental spines: (1) small, button-like spines and (2) sharply pointed spines. Partial sequences of the 28S rDNA and mitochondrial cox1 genes were generated and analysed to investigate the phylogenetic position of\n                    <jats:italic>Megacoelium<\/jats:italic>\n                    Szidat, 1954, within the Haploporidae Nicoll, 1914. Phylogenetic analyses placed\n                    <jats:italic>M. spinicavum<\/jats:italic>\n                    within the \u2018robust species\u2019 clade of\n                    <jats:italic>Saccocoelioides<\/jats:italic>\n                    Szidat, 1954, clustering with\n                    <jats:italic>S. bacilliformis<\/jats:italic>\n                    Szidat, 1973, although with weak support. These results provide additional evidence that\n                    <jats:italic>Saccocoelioides<\/jats:italic>\n                    is not monophyletic and support restricting the genus to the \u2018minute species\u2019 clade containing the type species. The \u2018robust species\u2019 clade appears to comprise at least three divergent lineages, potentially representing distinct genera, one of which includes\n                    <jats:italic>M. spinicavum.<\/jats:italic>\n                    The absence of molecular data for\n                    <jats:italic>M. plecostomi<\/jats:italic>\n                    Szidat, 1954, the type species of\n                    <jats:italic>Megacoelium<\/jats:italic>\n                    , continues to obscure its phylogenetic placement. We highlight the need for comprehensive morphological and multilocus molecular analyses, including SEM, to clarify the taxonomic status of\n                    <jats:italic>Megacoelium<\/jats:italic>\n                    and to resolve the evolutionary relationships of chalcinotrematine digeneans in Neotropical fishes.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x25101119","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:49:46Z","timestamp":1767858586000},"source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic assessment of\n                    <i>Megacoelium spinicavum<\/i>\n                    Thatcher &amp; Varella, 1981 (Digenea: Haploporidae) from\n                    <i>Pterygoplichthys pardalis<\/i>\n                    (Siluriformes: Loricariidae) in the Peruvian Amazon Basin"],"prefix":"10.1017","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3633-1365","authenticated-orcid":false,"given":"J.D.","family":"Chero","sequence":"first","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos (UNMSM)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"\u00d1acari","sequence":"additional","affiliation":[{"name":"Universidad de Antofagasta"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1959-4350","authenticated-orcid":false,"given":"J.","family":"Yunis-Aguinaga","sequence":"additional","affiliation":[{"name":"Universidad Cient\u00edfica del Sur"},{"name":"Instituto del Mar del Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6244-2654","authenticated-orcid":false,"given":"G.","family":"Murrieta","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de la Amazonia Peruana (IIAP)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Cruces","sequence":"additional","affiliation":[{"name":"Universidad Ricardo Palma (URP)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Huaman","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos (UNMSM)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.R.","family":"Cacique","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos (UNMSM)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos (UNMSM)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Mondrag\u00f3n-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos (UNMSM)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Mart\u00ednez-Rojas","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos (UNMSM)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"S0022149X25101119_r41","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780195135848.001.0001"},{"key":"S0022149X25101119_r45","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X1600047X"},{"key":"S0022149X25101119_r14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-8286.2002.00313.x"},{"key":"S0022149X25101119_r8","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2018.11.001"},{"key":"S0022149X25101119_r25","first-page":"129","volume-title":"Keys to the 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