{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T16:44:37Z","timestamp":1770050677036,"version":"3.49.0"},"reference-count":62,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"unspecified","delay-in-days":32,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human strongyloidiasis, caused by\n                    <jats:italic>Strongyloides stercoralis<\/jats:italic>\n                    , is a neglected disease of high worldwide prevalence, with considerable potential for severe, fatal outcomes in complicated cases. Studies using the rodent parasite\n                    <jats:italic>Strongyloides venezuelensis<\/jats:italic>\n                    as a model have provided valuable insights into strongyloidiasis, yet efficient, standardised methods for isolating large quantities of viable parasite eggs for biomedical research remain scarce. This study revisits and modernises the classical flotation principle, presenting a saturated-solution centrifugation protocol for egg recovery from infected clawed jirds (\n                    <jats:italic>Meriones unguiculatus<\/jats:italic>\n                    ). Saturated NaCl outperformed sucrose, primarily due to enhanced egg visualisation and reduced microbial contamination, achieving mean recovery of 84.8 \u00b1 6.7% (peaks to 94%). Key variables \u2013 including faecal suspension volume, solution concentration, reprocessing, and the NaCl gradient \u2013 were systematically optimised to maximise recovery and viability. The resulting protocol is cost-effective, rapid, and practical, enabling scalable collection of viable\n                    <jats:italic>S. venezuelensis<\/jats:italic>\n                    eggs (and likely other nematodes) for different applications, including hatching studies, larval development, microenvironmental assays, and drug screening. By integrating classical diagnostics with parametric optimisation, this study exemplifies how methodological advances preserve and renew foundational knowledge, underscoring its epistemological value in experimental parasitology.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x25101144","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T06:08:48Z","timestamp":1770012528000},"source":"Crossref","is-referenced-by-count":0,"title":["A century-old methodology revisited: Quantitative standardisation for collection of\n                    <i>Strongyloides venezuelensis<\/i>\n                    eggs and current applications"],"prefix":"10.1017","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8059-0332","authenticated-orcid":false,"given":"A.N.","family":"Sales","sequence":"first","affiliation":[{"name":"Universidade Federal de Lavras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3962-7359","authenticated-orcid":false,"given":"D.","family":"Pujoni","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7118-7210","authenticated-orcid":false,"given":"A.L.","family":"Melo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9976-6214","authenticated-orcid":false,"given":"V.L.T.","family":"Mati","sequence":"additional","affiliation":[{"name":"Universidade Federal de Lavras"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"S0022149X25101144_r39","doi-asserted-by":"publisher","DOI":"10.1007\/s10329-012-0302-x"},{"key":"S0022149X25101144_r56","doi-asserted-by":"publisher","DOI":"10.1186\/1476-7961-4-8"},{"key":"S0022149X25101144_r22","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1999.37.1.13"},{"key":"S0022149X25101144_r45","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2021.152129"},{"key":"S0022149X25101144_r31","doi-asserted-by":"publisher","DOI":"10.9771\/cmbio.v8i3.4476"},{"key":"S0022149X25101144_r6","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2002.40.4.181"},{"key":"S0022149X25101144_r10","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2019.01.010"},{"key":"S0022149X25101144_r60","doi-asserted-by":"publisher","DOI":"10.2307\/3283165"},{"key":"S0022149X25101144_r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2024.108801"},{"key":"S0022149X25101144_r19","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(69)90127-1"},{"key":"S0022149X25101144_r21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1993.tb00597.x"},{"key":"S0022149X25101144_r35","doi-asserted-by":"publisher","DOI":"10.24039\/rnh201482910"},{"key":"S0022149X25101144_r26","doi-asserted-by":"publisher","DOI":"10.2307\/3276396"},{"key":"S0022149X25101144_r59","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1921.tb60654.x"},{"key":"S0022149X25101144_r44","first-page":"326","article-title":"An ether sedimentation technique for routine stool examinations","volume":"8","author":"Ritchie","year":"1948","journal-title":"Bulletin of United States Army Medical Department"},{"key":"S0022149X25101144_r48","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X20000930"},{"key":"S0022149X25101144_r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.12.010"},{"key":"S0022149X25101144_r24","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.105291"},{"key":"S0022149X25101144_r12","doi-asserted-by":"publisher","DOI":"10.2307\/3281005"},{"key":"S0022149X25101144_r23","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-019-00186-x"},{"key":"S0022149X25101144_r36","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-014-0579-2"},{"key":"S0022149X25101144_r52","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2020.108046"},{"key":"S0022149X25101144_r58","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000082238"},{"key":"S0022149X25101144_r34","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X12000144"},{"key":"S0022149X25101144_r38","doi-asserted-by":"publisher","DOI":"10.9771\/cmbio.v5i1.4564"},{"key":"S0022149X25101144_r18","first-page":"50","article-title":"A new technique for counting nematode eggs in sheep faeces","volume":"12","author":"Gordon","year":"1939","journal-title":"Journal of the Council for Scientific and Industrial Research"},{"key":"S0022149X25101144_r42","first-page":"31","article-title":"A worm in the toilet bowl! 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