{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:28:02Z","timestamp":1785421682055,"version":"3.56.0"},"reference-count":45,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"unspecified","delay-in-days":97,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"DOI":"10.13039\/100024187","name":"Fundaci\u00f3n ArgenINTA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100024187","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Haemonchus contortus<\/jats:italic>\n                    is considered the most pathogenic gastrointestinal nematode of sheep and, due to the increasing resistance to synthetic anthelmintics, it poses a serious challenge to small ruminant production systems. The aim of this work was to evaluate the\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    anthelmintic activity of quebracho-chestnut tannin extract against\n                    <jats:italic>Haemonchus contortus<\/jats:italic>\n                    in lambs. The\n                    <jats:italic>in vitro<\/jats:italic>\n                    assays (egg hatching inhibition and larval migration inhibition) were performed to determine the effective concentrations required to inhibit 50% of egg hatching and larval migration (EC\n                    <jats:sub>50<\/jats:sub>\n                    ), which were 3.8 and 1.4 mg\/mL, respectively. For the\n                    <jats:italic>in vivo<\/jats:italic>\n                    trial, 18 lambs used were divided into three groups: Group 1 (control), Group 2 (a tannin dose equivalent to 0.3% of dry matter intake), and Group 3 (a tannin dose equivalent to 3% of dry matter intake). No significant differences (p &gt; 0.05) were observed among treatments in faecal egg counts, larval counts from coprocultures, or adult\n                    <jats:italic>H. contortus<\/jats:italic>\n                    recovery. The tannin extract was effective in inhibiting egg hatching and larval migration\n                    <jats:italic>in vitro<\/jats:italic>\n                    at low concentrations. However, when administered\n                    <jats:italic>in vivo<\/jats:italic>\n                    at 0.3% and 3% of dry matter intake, it failed to show any effect on faecal egg counts, larval counts in coproculture, or adult worm burden. Adult worms, eggs, and infective larvae were further examined using scanning electron microscopy. The observations revealed significant cuticular alterations in adult worms, which could potentially interfere with normal feeding and reproductive processes. Additionally, eggs and infective larvae exhibited shell rupture and cuticle damage.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x26101357","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T03:57:22Z","timestamp":1775620642000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>In vivo<\/i>\n                    and\n                    <i>in vitro<\/i>\n                    anthelmintic activity of quebracho-chestnut tannin extract against\n                    <i>Haemonchus contortus<\/i>\n                    in lambs"],"prefix":"10.1017","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6414-5885","authenticated-orcid":false,"given":"M. F.","family":"Bruttomesso","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wm52x94","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)"},{"name":"Instituto de Patobiolog\u00eda Veterinaria (IPVET), Unidad de Doble Dependencia, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1751-7298","authenticated-orcid":false,"given":"G.E.","family":"Morici","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wm52x94","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)"},{"name":"Instituto de Patobiolog\u00eda Veterinaria (IPVET), Unidad de Doble Dependencia, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.R.","family":"Arias","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wm52x94","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)"},{"name":"Instituto de Patobiolog\u00eda Veterinaria (IPVET), Unidad de Doble Dependencia, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4120-0458","authenticated-orcid":false,"given":"C.J.","family":"Garro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wm52x94","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)"},{"name":"Instituto de Patobiolog\u00eda Veterinaria (IPVET), Unidad de Doble Dependencia, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.I.","family":"Lobayan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/029efta16","id-type":"ROR","asserted-by":"publisher"}],"name":"Universidad del Salvador (USAL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9676-1602","authenticated-orcid":false,"given":"M.P.","family":"Palladino","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wm52x94","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)"},{"name":"Instituto de Patobiolog\u00eda Veterinaria (IPVET), Unidad de Doble Dependencia, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0319-0247","authenticated-orcid":false,"given":"F.A.","family":"Illanes","sequence":"additional","affiliation":[{"name":"Centro de Diagn\u00f3stico e Investigaciones Veterinarias (CEDIVE)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7103-2105","authenticated-orcid":false,"given":"J.H.","family":"Schapiro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wm52x94","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)"},{"name":"Instituto de Patobiolog\u00eda Veterinaria (IPVET), Unidad de Doble Dependencia, Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"S0022149X26101357_r3","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114599000501"},{"key":"S0022149X26101357_r12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.12.008"},{"key":"S0022149X26101357_r38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146021"},{"key":"S0022149X26101357_r41","first-page":"355","article-title":"Diagn\u00f3stico de resistencia a los antiparasitarios en rumiantes.\u00a0En: Rodr\u00edguez-Vivas, R. 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Tesis de doctorado, Universidad de la Rep\u00fablica. 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