{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:26:23Z","timestamp":1785432383071,"version":"3.56.0"},"reference-count":8,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"unspecified","delay-in-days":167,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pooling of gastropod samples is frequently used in surveys of the neurotropic nematode\n                    <jats:italic>Angiostrongylus cantonensis<\/jats:italic>\n                    to reduce cost and processing time. Here we highlight a potential limitation of this technique using the snail\n                    <jats:italic>Theba pisana<\/jats:italic>\n                    as an example. Pooled samples tested negative using a high-sensitivity qPCR assay, whereas subsequent individual testing revealed positive cases. This pattern is consistent with a dilution effect, where low parasite DNA concentrations could lead to false-negative results. These findings suggest that pooling may reduce diagnostic sensitivity in regions where infection intensities are low and should be applied with caution.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x26101588","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T12:12:04Z","timestamp":1781698324000},"source":"Crossref","is-referenced-by-count":0,"title":["When pooling fails: low-intensity\n                    <i>Angiostrongylus cantonensis<\/i>\n                    infections in\n                    <i>Theba pisana<\/i>\n                    result in false-negativity"],"prefix":"10.1017","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8742-0216","authenticated-orcid":false,"given":"L.","family":"Anettov\u00e1","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0415vcw02","id-type":"ROR","asserted-by":"publisher"}],"name":"Czech University of Life Sciences Prague: Ceska Zemedelska Univerzita v Praze"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6857-2553","authenticated-orcid":false,"given":"S.","family":"Jaume-Ramis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03e10x626","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Balearic Islands: Universitat de les Illes Balears"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Paredes-Esquivel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03e10x626","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Balearic Islands: Universitat de les Illes Balears"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Modry","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0415vcw02","id-type":"ROR","asserted-by":"publisher"}],"name":"Czech University of Life Sciences Prague: Ceska Zemedelska Univerzita v Praze"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"S0022149X26101588_r8","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1791"},{"key":"S0022149X26101588_r1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0013634"},{"key":"S0022149X26101588_r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2023.100610"},{"key":"S0022149X26101588_r6","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-025-01384-8"},{"key":"S0022149X26101588_r7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120686"},{"key":"S0022149X26101588_r2","first-page":"6","article-title":"Biology, systematics, life cycle, and distribution of Angiostrongylus cantonensis, the cause of rat lungworm disease","volume":"72","author":"Cowie","year":"2013","journal-title":"Hawai\u2019i Journal of Medicine and Public Health"},{"key":"S0022149X26101588_r4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0236849"},{"key":"S0022149X26101588_r3","doi-asserted-by":"publisher","DOI":"10.1111\/zph.13131"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X26101588","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T12:12:05Z","timestamp":1781698325000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X26101588\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":8,"alternative-id":["S0022149X26101588"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x26101588","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e66"}}