{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:43:35Z","timestamp":1785437015337,"version":"3.56.0"},"reference-count":35,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"unspecified","delay-in-days":167,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>In vivo<\/jats:italic>\n                    anthelmintic assay models are essential for evaluating candidate compounds in target species. Several rodent-helminth systems have been developed, with the Cobb-Wistar rat-\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    model emerging as promising for early-stage screening. Although this model has demonstrated utility, the development of an alternative model using a more widely accessible rat strain represents a valuable opportunity for advancing\n                    <jats:italic>in vivo<\/jats:italic>\n                    screening of anthelmintics. This study evaluated different immunosuppressants and regimens to identify treatments yielding the highest and most consistent worm burdens in experimentally infected Sprague-Dawley rats. Thirty rats were assigned to five groups (n = 6). Group 1 received unmedicated feed (negative control), while Groups 2, 3, and 4 received hydrocortisone acetate (HCA) in their diet at 60 ppm, 80 ppm, and a two-phase regimen of 200 ppm followed by 60 ppm, respectively. Group 5 received intramuscular methylprednisolone acetate (MPA) on three occasions. All animals were orally infected with\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    third-stage larvae, and necropsies were performed on Day 13 post-infection to recover worm burdens. Treatment had no effect on worm counts. The 80-ppm HCA group produced the highest mean burden with high variance; the 200:60 ppm group showed lower counts but less variance, and the 60-ppm HCA and MPA groups were comparable to controls. There was insufficient evidence to support the Sprague-Dawley rat-\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    model but, given the results of this study, further investigation and development are warranted.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x26101679","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T12:13:58Z","timestamp":1781698438000},"source":"Crossref","is-referenced-by-count":0,"title":["Investigating a Sprague-Dawley rat-based anthelmintic assay using\n                    <i>Trichostrongylus colubriformis<\/i>"],"prefix":"10.1017","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2363-3609","authenticated-orcid":false,"given":"Grethe","family":"Neethling","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009xwd568","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Free State"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5223-9513","authenticated-orcid":false,"given":"Daryl","family":"Codron","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009xwd568","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Free State"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8813-9549","authenticated-orcid":false,"given":"Luther","family":"van der Mescht","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009xwd568","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Free State"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"S0022149X26101679_r11","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2021.0142"},{"key":"S0022149X26101679_r22","unstructured":"Lenth, RV (2024) emmeans: Estimated Marginal Means, aka Least-Squares Means. R package version 1.9.5. Available at: https:\/\/CRAN.R-project.org\/package=emmeans (accessed 5 November 2025)."},{"key":"S0022149X26101679_r32","first-page":"69","article-title":"Prevalence of endoparasitic infections among rats in laboratory animal facility and management","volume":"12","author":"Vellayan","year":"2021","journal-title":"Malaysian Journal of Veterinary Research"},{"key":"S0022149X26101679_r28","unstructured":"R Core Team (2022) R: A language and environment for statistical computing. Version 4.2. Vienna: R Foundation for Statistical Computing. Available at: https:\/\/www.r-project.org\/ (accessed 5 November 2025)."},{"key":"S0022149X26101679_r1","doi-asserted-by":"publisher","DOI":"10.2307\/3277866"},{"key":"S0022149X26101679_r17","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(76)90066-2"},{"key":"S0022149X26101679_r25","unstructured":"Mills, BJ , McTier, TL , Knauer, CS and Woods, DJ (2020) Use of compounds for treating parasitic infections. WO Patent WO2020146338A1, 17 September 2020."},{"key":"S0022149X26101679_r29","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-6-153"},{"key":"S0022149X26101679_r33","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"S0022149X26101679_r15","unstructured":"Hartig, F (2022) DHARMa: Residual Diagnostics for Hierarchical (Multi-Level \/ Mixed) Regression Models. R package version 0.4.7. 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