{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:04:41Z","timestamp":1789718681544,"version":"4.0.1"},"reference-count":77,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"unspecified","delay-in-days":260,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["11220200101713CO"],"award-info":[{"award-number":["11220200101713CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2020-SERIEA-01531"],"award-info":[{"award-number":["PICT-2020-SERIEA-01531"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Morphologically similar diplostomid metacercariae were found infecting four species of Pampean rivulid fishes \u2013\n                    <jats:italic>Austrolebias bellottii<\/jats:italic>\n                    ,\n                    <jats:italic>Amatolebias patriciae<\/jats:italic>\n                    ,\n                    <jats:italic>Argolebias nigripinnis<\/jats:italic>\n                    , and\n                    <jats:italic>Titanolebias elongatus<\/jats:italic>\n                    \u2013 from temporary freshwater ponds in the Pampean region of Argentina. Although these larvae occur in different host species and localities, comparative morphological analyses revealed extensive overlap in diagnostic characters. To assess whether this morphological similarity reflected conspecificity, molecular analyses based on partial 28S rDNA and ITS2 rDNA sequences were performed. Phylogenetic reconstructions recovered all metacercariae from rivulid hosts as a single, well-supported lineage, consistent with their assignment to a single lineage\n                    <jats:italic>Posthodiplostomum<\/jats:italic>\n                    species. Genetic distances among ITS2 sequences were zero, indicating complete sequence identity across hosts and localities. Infection parameters varied among host species and populations, with\n                    <jats:italic>Austrolebias bellottii<\/jats:italic>\n                    and\n                    <jats:italic>Titanolebias elongatus<\/jats:italic>\n                    exhibiting the highest values. Differences in infection levels could reflect variation in local environmental conditions, habitat characteristics, and the availability of avian definitive hosts, potentially influencing parasite transmission dynamics. This study provides integrative morphological and molecular evidence consistent with all metacercariae representing a single\n                    <jats:italic>Posthodiplostomum<\/jats:italic>\n                    lineage infecting multiple Pampean rivulid species and contributes to a better understanding of parasite diversity and transmission patterns in South American temporary ponds.\n                  <\/jats:p>","DOI":"10.1017\/s0022149x26101862","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:59:15Z","timestamp":1789718355000},"source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic relationships of\n                    <i>Posthodiplostomum<\/i>\n                    sp. metacercarial morphotype (Diplostomidae) infecting four Pampean rivulid genera"],"prefix":"10.1017","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-333X","authenticated-orcid":false,"given":"M.M.","family":"Montes","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02a53x034","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE; CONICET-UNLP-CICPBA)"},{"name":"Killifish Foundation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Barneche","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02a53x034","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE; CONICET-UNLP-CICPBA)"},{"name":"Killifish Foundation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8211-4135","authenticated-orcid":false,"given":"T.","family":"Acu\u00f1a Gonz\u00e1lez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02a53x034","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE; CONICET-UNLP-CICPBA)"},{"name":"Killifish Foundation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0454-2603","authenticated-orcid":false,"given":"I.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Killifish Foundation"},{"id":[{"id":"https:\/\/ror.org\/01tjs6929","id-type":"ROR","asserted-by":"publisher"}],"name":"Institulo de Limnolog\u00eda (ILPLA, CONCIET-UNLP-CICPBA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7227-2732","authenticated-orcid":false,"given":"F.","family":"Alonso","sequence":"additional","affiliation":[{"name":"Killifish Foundation"},{"id":[{"id":"https:\/\/ror.org\/00htwgm11","id-type":"ROR","asserted-by":"publisher"}],"name":"Institulo de Bio y Geociencias (IBIGEO; CONICET-UNSa)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8612-3057","authenticated-orcid":false,"given":"M.","family":"Ib\u00e1\u00f1ez Shimabukuro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02a53x034","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE; CONICET-UNLP-CICPBA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Reig Cardarella","sequence":"additional","affiliation":[{"name":"Killifish Foundation"},{"id":[{"id":"https:\/\/ror.org\/00htwgm11","id-type":"ROR","asserted-by":"publisher"}],"name":"Escuela de Tecnolog\u00eda M\u00e9dica y Centro Integrativo de Biolog\u00eda y Qu\u00edmica Aplicada (CIBQA). Universidad Bernardo O\u2019 Higgins"},{"id":[{"id":"https:\/\/ror.org\/03gtdcg60","id-type":"ROR","asserted-by":"publisher"}],"name":"Universidad Diego Portales"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"S0022149X26101862_r35","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"S0022149X26101862_r7","first-page":"185","article-title":"Biodiversity of fish parasites from Guandu River, Southeastern Brazil: an ecological approach","volume":"5","author":"Azevedo","year":"2011","journal-title":"Neotropical Helminthology"},{"key":"S0022149X26101862_r41","doi-asserted-by":"publisher","DOI":"10.3897\/biorisk.5.849"},{"key":"S0022149X26101862_r33","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090228"},{"key":"S0022149X26101862_r65","doi-asserted-by":"publisher","DOI":"10.1645\/12-170.1"},{"key":"S0022149X26101862_r42","doi-asserted-by":"publisher","DOI":"10.1590\/S1519-69842011000400006"},{"key":"S0022149X26101862_r71","doi-asserted-by":"publisher","DOI":"10.1214\/aos\/1176344136"},{"key":"S0022149X26101862_r62","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.116194"},{"key":"S0022149X26101862_r53","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780195135848.001.0001"},{"key":"S0022149X26101862_r30","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.243160"},{"key":"S0022149X26101862_r52","doi-asserted-by":"publisher","DOI":"10.22370\/rbmo.2019.54.3.2015"},{"key":"S0022149X26101862_r43","first-page":"1008","article-title":"What do we know about the effects of pesticides on helminths?","volume":"2","author":"Monte","year":"2017","journal-title":"JSM Biology"},{"key":"S0022149X26101862_r72","doi-asserted-by":"publisher","DOI":"10.1525\/auk.2008.125.1.20"},{"key":"S0022149X26101862_r56","first-page":"121","article-title":"Sobre el ciclo biol\u00f3gico de Posthodiplostomum nanum Dubois, 1937 (Trematoda, Diplostomatidae)","volume":"32","author":"de N\u00fa\u00f1ez M","year":"1973","journal-title":"Physis"},{"key":"S0022149X26101862_r4","doi-asserted-by":"publisher","DOI":"10.1093\/zoolinnean\/zlad032"},{"key":"S0022149X26101862_r75","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025546001611"},{"key":"S0022149X26101862_r59","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182021001748"},{"key":"S0022149X26101862_r27","unstructured":"Froes, R and Pauly, D (Eds) (2021) Titanolebias elongatus . FishBase. Available at: https:\/\/www.fishbase.se\/summary\/Austrolebias-elongatus"},{"key":"S0022149X26101862_r2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01092"},{"key":"S0022149X26101862_r32","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46091-4"},{"key":"S0022149X26101862_r66","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/sys029"},{"key":"S0022149X26101862_r14","first-page":"69","article-title":"Origin of species diversity in the catfish genus Hypostomus (Siluriformes: Loricariidae) inhabiting the Paran\u00e1 River basin, with the description of a new species","volume":"4983","author":"Cardoso","year":"2021","journal-title":"Zootaxa"},{"key":"S0022149X26101862_r22","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-024-08420-w"},{"key":"S0022149X26101862_r8","doi-asserted-by":"publisher","DOI":"10.1007\/s10750-007-9246-3"},{"key":"S0022149X26101862_r54","doi-asserted-by":"publisher","DOI":"10.1645\/12-84.1"},{"key":"S0022149X26101862_r6","first-page":"403","article-title":"Ecologia da comunidade de metazo\u00e1rios parasitos do Acar\u00e1 Geophagus brasiliensis (Quoy e Gaimard, 1824) (Preciformes: Cichlidae) do Rio Guandu, Estado do Rio de Janeiro, Brasil","volume":"28","author":"Azevedo","year":"2006","journal-title":"Acta Scientiarum. Biological Sciences"},{"key":"S0022149X26101862_r57","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-018-1320-y"},{"key":"S0022149X26101862_r31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707763104"},{"key":"S0022149X26101862_r64","first-page":"369","article-title":"Helminth parasites of alien freshwater fishes in Patagonia (Argentina)","volume":"7","author":"Rauque","year":"2018","journal-title":"International Journal for Parasitology: Parasites and Wildlife"},{"key":"S0022149X26101862_r77","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2023.008"},{"key":"S0022149X26101862_r34","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx108"},{"key":"S0022149X26101862_r12","first-page":"289","article-title":"Nematodes of freshwater fishes of the Neotropical region","volume":"38","author":"Bunkley-Williams","year":"2002","journal-title":"Caribbean Journal of Science"},{"key":"S0022149X26101862_r51","doi-asserted-by":"publisher","DOI":"10.3390\/cli9030040"},{"key":"S0022149X26101862_r70","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"S0022149X26101862_r16","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X25000252"},{"key":"S0022149X26101862_r21","unstructured":"Delgado, C and Garc\u00eda, G (2015) Coevolution between Contracaecum (Nematoda, Anisakidae) and Austrolebias (Cyprinodontiformes, Rivulidae) host-parasite complex from SW Atlantic coastal basins. Noticias de la Sociedad Zool\u00f3gica del Uruguay. URL https:\/\/www.szu.org.uy\/noticias\/noticias_szu_08_28.pdf (consultado junio de 2026)."},{"key":"S0022149X26101862_r17","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2017.06.007"},{"key":"S0022149X26101862_r39","first-page":"228","article-title":"Geometric morphometric on a new species of Trichodinidae. A tool to discriminate trichodinid species combined with traditional morphology and molecular analysis","volume":"7","author":"Marcotegui","year":"2018","journal-title":"International Journal for Parasitology: Parasites and Wildlife"},{"key":"S0022149X26101862_r74","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007000248"},{"key":"S0022149X26101862_r40","doi-asserted-by":"publisher","DOI":"10.1111\/cobi.70104"},{"key":"S0022149X26101862_r15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0202024"},{"key":"S0022149X26101862_r55","doi-asserted-by":"publisher","DOI":"10.1079\/9780851995472.0167"},{"key":"S0022149X26101862_r50","doi-asserted-by":"publisher","DOI":"10.22201\/ib.20078706e.2019.90.2652"},{"key":"S0022149X26101862_r49","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200007709X"},{"key":"S0022149X26101862_r18","first-page":"29","volume-title":"Una aproximaci\u00f3n a la realidad regional de la Argentina: teor\u00eda y metodolog\u00eda para una mirada problematizadora y cr\u00edtica","author":"Cieza","year":"2025"},{"key":"S0022149X26101862_r69","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2427.2008.02072.x"},{"key":"S0022149X26101862_r78","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2010.0055"},{"key":"S0022149X26101862_r60","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00117-X"},{"key":"S0022149X26101862_r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcz.2025.04.009"},{"key":"S0022149X26101862_r68","doi-asserted-by":"publisher","DOI":"10.2478\/s11686-011-0081-3"},{"key":"S0022149X26101862_r20","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00127-1"},{"key":"S0022149X26101862_r13","doi-asserted-by":"publisher","DOI":"10.2307\/3284227"},{"key":"S0022149X26101862_r29","doi-asserted-by":"publisher","DOI":"10.1111\/oik.07414"},{"key":"S0022149X26101862_r44","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X18000172"},{"key":"S0022149X26101862_r67","first-page":"121","article-title":"Parasites community of Astronotus crassipinnis (Cichlidae), a fish from the Brazilian Amazon","volume":"64","author":"Santos","year":"2018","journal-title":"Annals of Parasitology"},{"key":"S0022149X26101862_r9","doi-asserted-by":"publisher","DOI":"10.1201\/b19016"},{"key":"S0022149X26101862_r10","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X16000298"},{"key":"S0022149X26101862_r24","doi-asserted-by":"publisher","DOI":"10.1080\/0307945021000005815"},{"key":"S0022149X26101862_r79","unstructured":"WoRMS (2026) Posthodiplostomum Dubois, 1936. World Register of Marine Species. URL https:\/\/www.marinespecies.org\/aphia.php?p=taxdetails&id=108682 (consultado el 20 de junio de 2026)."},{"key":"S0022149X26101862_r61","first-page":"257","article-title":"Infec\u00e7\u00e3o natural de Poecilia reticulata (Actinopterygii: Poeciliidae) por metacerc\u00e1rias na represa da Pampulha, Belo Horizonte, Minas Gerais, Brasil","volume":"38","author":"Pinto","year":"2012","journal-title":"Boletim do Instituto de Pesca"},{"key":"S0022149X26101862_r28","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12194"},{"key":"S0022149X26101862_r46","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X24000658"},{"key":"S0022149X26101862_r37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2008.02059.x"},{"key":"S0022149X26101862_r48","first-page":"125","article-title":"New data on the acanthocephalan Neoechinorhynchus villoldoi Vizca\u00edno, 1992 (Neoechinorhynchidae: Acanthocephala), based on specimens found in Austrolebias bellottii (Steindachner, 1881) (Rivulidae: Cyprinodontiformes) from Punta Indio, Argentina","volume":"11","author":"Montes","year":"2017","journal-title":"Neotropical Helminthology"},{"key":"S0022149X26101862_r25","unstructured":"Fernandes, BMM , Justo, MCN , C\u00e1rdenas, MQ and Cohen, SC (2015) South American trematodes parasites of birds and mammals. Rio de Janeiro, Biblioteca de Ci\u00eancias Biom\u00e9dicas, ICICT, Fiocruz\u2013RJ."},{"key":"S0022149X26101862_r38","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2005.010"},{"key":"S0022149X26101862_r47","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2026.103291"},{"key":"S0022149X26101862_r63","first-page":"141","volume-title":"Technical Bulletin 1","author":"Pritchard","year":"1982"},{"key":"S0022149X26101862_r23","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2005.03.004"},{"key":"S0022149X26101862_r58","first-page":"59","article-title":"Nematode parasite of Cynolebias bellotti (Pisces: Cyprinodonta)","volume":"4","author":"Perea","year":"1933","journal-title":"Revista de Biologia e Higiene"},{"key":"S0022149X26101862_r19","first-page":"33","article-title":"Phylogeny and classification of Rivulidae revisited: origin and evolution of annualism and miniaturization in rivulid fishes (Cyprinodontiformes: Aplocheiloidei)","volume":"3","author":"Costa","year":"1998","journal-title":"Journal of Comparative Biology"},{"key":"S0022149X26101862_r1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpvbd.2021.100051"},{"key":"S0022149X26101862_r3","first-page":"1","article-title":"Los peces estacionales y las especies de Argentina","volume":"10","author":"Alonso","year":"2020","journal-title":"Temas de Biolog\u00eda y Geolog\u00eda del Noroeste Argentino"},{"key":"S0022149X26101862_r36","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2017.06.003"},{"key":"S0022149X26101862_r73","doi-asserted-by":"publisher","DOI":"10.15446\/abc.v22n2.60015"},{"key":"S0022149X26101862_r76","doi-asserted-by":"publisher","DOI":"10.15560\/16.5.1277"},{"key":"S0022149X26101862_r11","first-page":"1","article-title":"Impacto del cambio clim\u00e1tico: un an\u00e1lisis espacial del riesgo futuro al cambio clim\u00e1tico en el sur de la Regi\u00f3n Pampeana (Argentina)","volume":"69","author":"Brendel","year":"2024","journal-title":"Papeles de Geograf\u00eda"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X26101862","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:59:20Z","timestamp":1789718360000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X26101862\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":77,"alternative-id":["S0022149X26101862"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x26101862","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e108"}}