{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:34:18Z","timestamp":1760016858165},"reference-count":11,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":9515,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["J. Hyg."],"published-print":{"date-parts":[[1983,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The detection of<jats:italic>Legionella pneumophila<\/jats:italic>in water via guinea-pig intraperitoneal injection has been compared with direct isolation of these organisms on semiselective BCYE\u03b1 media. Both techniques were of similar sensitivity, detecting as few as 10<jats:sup>2<\/jats:sup><jats:italic>L. pneumophila<\/jats:italic>in 10 ml of water. However, at this concentration, detection using guinea-pig intraperitoneal injection was via the indirect parameters of antibody production and immunofluorescent microscopy. Isolation of<jats:italic>L. pneumophila<\/jats:italic>from guinea-pig tissue for further investigation required &gt; 10<jats:sup>7<\/jats:sup>organisms injected and in this respect is relatively much less sensitive than direct isolation on semiselective BCYE\u03b1 media. Since<jats:italic>L. micdadei<\/jats:italic>and<jats:italic>L. longbeachae<\/jats:italic>are inhibited by one of the selective supplements used, other supplements and techniques need to be developed in order to avoid possible inhibition of other<jats:italic>Legionella<\/jats:italic>species.<\/jats:p>","DOI":"10.1017\/s0022172400060186","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T13:01:37Z","timestamp":1255957297000},"page":"179-187","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":13,"title":["Isolation of<i>Legionella pneumophila<\/i>from water supplies: comparison of methods based on the guinea-pig and culture media"],"prefix":"10.1017","volume":"91","author":[{"given":"R. B.","family":"Fitzgeorge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J.","family":"Dennis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,10,19]]},"reference":[{"key":"S0022172400060186_ref009","first-page":"1902","article-title":"Isolation of Legionella pneumophila from cooling tower water by filtration","volume":"14","author":"Orrison","year":"1981","journal-title":"Applied Environmental Microbiology"},{"key":"S0022172400060186_ref004","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1017\/S0022172400063877","article-title":"Aerosol infection of animals with strains of Legionella pneumophilaof different virulence: comparison with intrnperitoneal and intranasal routes of infection","volume":"90","author":"Fitzoeoroe","year":"1983","journal-title":"Journal of Hygiene"},{"key":"S0022172400060186_ref011","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1128\/AEM.42.5.768-772.1981","article-title":"Glycine containing selective medium for isolation of Legionellacae from environmental water supplies","volume":"14","author":"Wadowsky","year":"1981","journal-title":"Journal of Applied and Environmental Microbiology"},{"key":"S0022172400060186_ref010","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1136\/bmj.282.6263.515","article-title":"Isolation of Legionella pnemnophila from water systems: methods and preliminary results","volume":"282","author":"Town","year":"1981","journal-title":"British Medical Journal"},{"key":"S0022172400060186_ref008","volume-title":"The Accredition and Recognition Schemes for Supplies of Laboratory Animals","year":"1974"},{"key":"S0022172400060186_ref002","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/JCM.14.3.298-303.1981","article-title":"Improved semiselective medium for isolation of L. pneumophilafrom contaminated clinical and environmental specimens","volume":"14","author":"Edelstein","year":"1981","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022172400060186_ref005","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1128\/AEM.41.1.9-16.1981","article-title":"Ecological distribution of Legionella pneumophila","volume":"41","author":"Fliermans","year":"1981","journal-title":"Applied Environmental Microbiology"},{"key":"S0022172400060186_ref006","first-page":"101","article-title":"Isolation of Legionella pneumophila from clinical specimens: salutary effects of lung tissue dilution","volume":"122","author":"Lattimer","year":"1980","journal-title":"American review of respiratory diseases"},{"key":"S0022172400060186_ref007","first-page":"13","volume-title":"Legionnaires11 Disease","author":"Lattimer","year":"1981"},{"key":"S0022172400060186_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)91944-4"},{"key":"S0022172400060186_ref003","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1128\/JCM.15.6.1092-1096.1982","article-title":"Isolation of L. pneumophilafrom hospital potable water specimens: comparison of direct plating with guinea-pig inoculaton","volume":"15","author":"Edelstein","year":"1982","journal-title":"Journal of Clinical Microbiology"}],"container-title":["Journal of Hygiene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022172400060186","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T03:47:24Z","timestamp":1590205644000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022172400060186\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,10]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,10]]}},"alternative-id":["S0022172400060186"],"URL":"https:\/\/doi.org\/10.1017\/s0022172400060186","relation":{},"ISSN":["0022-1724"],"issn-type":[{"value":"0022-1724","type":"print"}],"subject":[],"published":{"date-parts":[[1983,10]]},"assertion":[{"value":"Cambridge University Press 1983","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}