{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:39:42Z","timestamp":1760888382446},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":8845,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["J. Hyg."],"published-print":{"date-parts":[[1985,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Foods from outbreaks of food poisoning were examined for the presence of staphylococcal enterotoxin A (SEA) by a sandwich ELISA using microtitre trays as the solid phase and SEA antibodies raised in sheep.<\/jats:p><jats:p>The presence of SEA was confirmed by neutralization tests. The toxin was detected in 12 of 15 foods from separate outbreaks of staphylococcal food poisoning; all 15 foods contained a strain of<jats:italic>Staphylococcus aureus<\/jats:italic>which produced SEA. For most foods a simple extraction procedure without a concentration step was sufficient to detect the toxin. The method was semi-quantitative and recoveries of SEA added to control foods varied from 30 to 80%. The foods from outbreaks contained between 1 and 10 \u03bcg of SEA\/100 g. SEA was not found in foods from 21 outbreaks in which an SEA-producing strain of<jats:italic>Staph. aureus<\/jats:italic>was not isolated.<\/jats:p>","DOI":"10.1017\/s0022172400062367","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T12:53:34Z","timestamp":1255956814000},"page":"131-138","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":13,"title":["The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning"],"prefix":"10.1017","volume":"95","author":[{"given":"Antonnette A.","family":"Wieneke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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(1980). Application of ELISA for the Diagnosis and Epidemiology of Hepatitis A, pp. 85\u201386. (Thesis, University of Utrecht). Amsterdam: Rodopi."},{"key":"S0022172400062367_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400022592"},{"key":"S0022172400062367_ref012","volume-title":"Proceedings of 5th International Symposium on Staphylococci and Staphylococcal Infections","author":"Igarashi","year":"1984"},{"key":"S0022172400062367_ref013","first-page":"1138","article-title":"Enzyme immunoassay for staphylococcal enterotoxin A","volume":"63","author":"Kauffman","year":"1980","journal-title":"Journal of the Association of Official Analytical Chemists"},{"key":"S0022172400062367_ref006","first-page":"173","volume-title":"Isolation and Identification Methods for Food Poisoning Organisms","author":"de Saxe","year":"1982"},{"key":"S0022172400062367_ref010","first-page":"273","volume-title":"The Microbiological Safety of Food","author":"Gilbert","year":"1973"},{"key":"S0022172400062367_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90074-6"},{"key":"S0022172400062367_ref021","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1128\/jcm.8.5.473-479.1978","article-title":"Simple assay for staphylococcal enterotoxins A, B and C: modification of enzyme-linked immunosorbent assay","volume":"8","author":"Stiffler-Rosenberg","year":"1978","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022172400062367_ref009","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/AEM.44.6.1349-1355.1982","article-title":"Enzyme-linked immuriosorbent assay for detection of staphylococcal enterotoxins in foods","volume":"44","author":"Freed","year":"1982","journal-title":"Applied and Environmental Microbiology"}],"container-title":["Journal of Hygiene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022172400062367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T16:00:22Z","timestamp":1634054422000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022172400062367\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,8]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985,8]]}},"alternative-id":["S0022172400062367"],"URL":"https:\/\/doi.org\/10.1017\/s0022172400062367","relation":{},"ISSN":["0022-1724"],"issn-type":[{"value":"0022-1724","type":"print"}],"subject":[],"published":{"date-parts":[[1985,8]]},"assertion":[{"value":"Cambridge University Press 1985","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}