{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:24:11Z","timestamp":1787066651574,"version":"build-2736575974"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":9149,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["J. Hyg."],"published-print":{"date-parts":[[1984,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Features are described of a new staining method for the detection of\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    oocysts in faeces. Smears, fixed in acid-methanol, are stained with heated safranin and counterstained with methylene blue. Oocysts stain a vivid orange-pink and are easily recognized. The method is rapid and simple with little source of error. The method is more sensitive than currently recommended Ziehl-Neelson methods; of 26 cases of cryptosporidiosis diagnosed by the new method only 19 were detected by acid-fast and only 11 by acid-alcohol-fast methods. Oocysts can be stained by safranin-methylene blue after concentration by various methods, in paraffin-embedded material, and after storage for months at various temperatures.\n                  <\/jats:p>","DOI":"10.1017\/s0022172400064858","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T08:57:16Z","timestamp":1255942636000},"page":"317-323","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":113,"title":["The development and performance of a simple, sensitive method for the detection of\n                    <i>Cryptosporidium<\/i>\n                    oocysts in faeces"],"prefix":"10.1017","volume":"93","author":[{"given":"Derrick","family":"Baxby","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Blundell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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