{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:52:57Z","timestamp":1762865577887},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":8419,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["J. Hyg."],"published-print":{"date-parts":[[1986,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Restriction enzyme fingerprints were generated from purified plasmid DNA from 324 clinical isolates that belonged to 7 enterobacterial genera and 88 single plasmids in <jats:italic>Escherichia coli<\/jats:italic> K12 according to the following strategy.<\/jats:p><jats:p>Purified plasmid DNA was digested with <jats:italic>PstI.<\/jats:italic> The number of fragments detected in a 0\u00b78 agarose gel was used to determine which 2 of 6 restriction enzymes including <jats:italic>Pstl<\/jats:italic> was most likely to provide a fingerprint comprising sufficient fragments to ensure specificity but sufficiently few to allow easy visual assessment and minimize coincidental matching. When <jats:italic>PstI<\/jats:italic> produced &gt; 20 fragments, <jats:italic>Eco<\/jats:italic> RI and <jats:italic>HindIII<\/jats:italic> were used; when <jats:italic>PstI<\/jats:italic> generated &lt; 6 fragments <jats:italic>Bsp<\/jats:italic> 1286 and <jats:italic>AvaII<\/jats:italic> were used and <jats:italic>SmaI<\/jats:italic> was employed when between 6 and 20 fragments were obtained from <jats:italic>PstI<\/jats:italic> digests. Using a minimum of 12 fragments from a combination of 2 enzymes as the criterion for characterizing a strain\/plasmid, satisfactory 2-enzyme fingerprints were obtained from 87% of the strains and plasmids studied using <jats:italic>PstI<\/jats:italic> and no more than two additional enzymes per strain. Of the remaining 54 strains, 51 harboured only small plasmids (&lt; 10 kb) and 3 produced satisfactory fingerprints when digested with a fourth enzyme.<\/jats:p>","DOI":"10.1017\/s0022172400065281","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T12:49:38Z","timestamp":1255956578000},"page":"205-210","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":25,"title":["Restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application"],"prefix":"10.1017","volume":"97","author":[{"given":"D. J.","family":"Platt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. S.","family":"Chesham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. A.","family":"Kraft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Taggart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,10,19]]},"reference":[{"key":"S0022172400065281_ref006","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-17-1-79"},{"key":"S0022172400065281_ref003","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-18-2-277"},{"key":"S0022172400065281_ref004","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-16-4-433"},{"key":"S0022172400065281_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0195-6701(81)90066-9"},{"key":"S0022172400065281_ref011","first-page":"273","volume-title":"Microbiology","author":"Richmond","year":"1978"},{"key":"S0022172400065281_ref013","doi-asserted-by":"publisher","DOI":"10.1007\/BF00264835"},{"key":"S0022172400065281_ref005","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-18-1-95"},{"key":"S0022172400065281_ref009","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-21-4-325"},{"key":"S0022172400065281_ref007","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198207013070101"},{"key":"S0022172400065281_ref001","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-20-1-39"},{"key":"S0022172400065281_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(83)90028-8"},{"key":"S0022172400065281_ref012","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/143.2.170"},{"key":"S0022172400065281_ref008","first-page":"107","article-title":"Bacterial plasmids and their fingerprints","volume":"1","author":"Platt","year":"1983","journal-title":"Disease Markers"}],"container-title":["Journal of Hygiene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022172400065281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T03:40:47Z","timestamp":1558496447000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022172400065281\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,10]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,10]]}},"alternative-id":["S0022172400065281"],"URL":"https:\/\/doi.org\/10.1017\/s0022172400065281","relation":{},"ISSN":["0022-1724"],"issn-type":[{"value":"0022-1724","type":"print"}],"subject":[],"published":{"date-parts":[[1986,10]]},"assertion":[{"value":"Cambridge University Press 1986","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}