{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T01:15:40Z","timestamp":1775438140067,"version":"3.50.1"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2007,6,25]],"date-time":"2007-06-25T00:00:00Z","timestamp":1182729600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Laryngol. Otol."],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0022215107009097_sec1\"><jats:title>Objectives:<\/jats:title><jats:p>To develop and characterise an experimental model of recurrent laryngeal nerve injury for the study of viral gene therapy.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215107009097_sec2\"><jats:title>Methods:<\/jats:title><jats:p>Twenty rats underwent unilateral recurrent laryngeal nerve injury. After vocal fold mobility was observed, larynges were serially sectioned, and immunohistochemical techniques were employed to stain for neurofilament and motor endplates in order for a blinded investigator to determine the percentage of nerve\u2013endplate contact, as a histological indicator of an intact neuromuscular connection.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215107009097_sec3\"><jats:title>Results:<\/jats:title><jats:p>All animal procedures resulted in complete, ipsilateral vocal fold paralysis that recovered by three weeks. The mean nerve\u2013endplate contact percentage was 11.6 per cent at one week, 53.9 per cent at two weeks, 88.6 per cent at three weeks, 81.7 per cent at four weeks and 86.6 per cent at five weeks. The differences between results at week one and week three were statistically significant (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01). The mean nerve\u2013endplate contact percentage on the control side was 86.8 per cent.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215107009097_sec4\"><jats:title>Conclusions:<\/jats:title><jats:p>There was a dramatic, measurable decrease in nerve\u2013endplate contact percentage following crush injury to the recurrent laryngeal nerve. Spontaneous recovery was observed by three weeks post-injury. This model will be used to investigate the potential therapeutic role of viral gene therapy for the treatment of recurrent laryngeal nerve injury.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s0022215107009097","type":"journal-article","created":{"date-parts":[[2007,6,25]],"date-time":"2007-06-25T13:34:04Z","timestamp":1182778444000},"page":"500-505","source":"Crossref","is-referenced-by-count":5,"title":["Development and characterisation of an experimental recurrent laryngeal nerve injury model for the study of viral gene therapy"],"prefix":"10.1017","volume":"122","author":[{"given":"K","family":"Fung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N D","family":"Hogikyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S B","family":"Heavner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Ekbom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E L","family":"Feldman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,6,25]]},"reference":[{"key":"S0022215107009097_ref2","doi-asserted-by":"publisher","DOI":"10.1067\/mhn.2002.123546"},{"key":"S0022215107009097_ref8","first-page":"S110","article-title":"Adeno-associated viral gene expression in the adult rat spinal cord following remote vector delivery","volume":"1","author":"Boulis","year":"2000","journal-title":"Mol Ther"},{"key":"S0022215107009097_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012397570-6\/50015-0"},{"key":"S0022215107009097_ref15","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199107000-00003"},{"key":"S0022215107009097_ref9","first-page":"1308","article-title":"Retrograde adenoviral gene delivery to rat spinal cord via sciatic nerve microinjection","volume":"24","author":"Boulis","year":"1998","journal-title":"Soc Neurosci Abstr"},{"key":"S0022215107009097_ref12","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1994.1104"},{"key":"S0022215107009097_ref10","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200111000-00032"},{"key":"S0022215107009097_ref14","doi-asserted-by":"publisher","DOI":"10.3109\/00016489509139307"},{"key":"S0022215107009097_ref18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302665"},{"key":"S0022215107009097_ref4","first-page":"132","article-title":"CNS gene delivery by retrograde transport of recombinant replication-defective adenoviruses","volume":"2","author":"Ghadge","year":"1995","journal-title":"Gene Ther"},{"key":"S0022215107009097_ref5","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200306000-00013"},{"key":"S0022215107009097_ref11","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.125.5.555"},{"key":"S0022215107009097_ref1","first-page":"423","volume-title":"Complications in Head and Neck Surgery","author":"Eisele","year":"2000"},{"key":"S0022215107009097_ref13","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19981215)54:6<766::AID-JNR4>3.0.CO;2-A"},{"key":"S0022215107009097_ref16","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890160035007"},{"key":"S0022215107009097_ref7","first-page":"1309","article-title":"Remote adenoviral gene delivery to the spinal cord: contralateral delivery and reinjection","volume":"48","author":"Turner","year":"2001","journal-title":"Neurosurgery"},{"key":"S0022215107009097_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03933-1"},{"key":"S0022215107009097_ref6","first-page":"131","article-title":"Characterization of adenoviral gene expression in spinal cord after remote vector delivery","volume":"45","author":"Boulis","year":"1999","journal-title":"Neurosurgery"}],"container-title":["The Journal of Laryngology &amp; Otology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022215107009097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T23:47:55Z","timestamp":1556668075000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022215107009097\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,25]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["S0022215107009097"],"URL":"https:\/\/doi.org\/10.1017\/s0022215107009097","relation":{},"ISSN":["0022-2151","1748-5460"],"issn-type":[{"value":"0022-2151","type":"print"},{"value":"1748-5460","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,25]]}}}