{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:55:46Z","timestamp":1791330946440,"version":"4.3.1"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"S1","license":[{"start":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T00:00:00Z","timestamp":1344988800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Laryngol. Otol."],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0022215112001661_sec1\"><jats:title>Background:<\/jats:title><jats:p>Chronic tympanic membrane perforations can cause significant morbidity. The term myringoplasty describes the operation used to close such perforations. A variety of graft materials are available for use in myringoplasty, but all have limitations and few studies report post-operative hearing outcomes. Recently, the biomedical applications of silk fibroin protein have been studied. This material's biocompatibility, biodegradability and ability to act as a scaffold to support cell growth prompted an investigation of its interaction with human tympanic membrane keratinocytes.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215112001661_sec2\"><jats:title>Methods and materials:<\/jats:title><jats:p>Silk fibroin membranes were prepared and human tympanic membrane keratinocytes cultured. Keratinocytes were seeded onto the membranes and immunostained for a number of relevant protein markers relating to cell proliferation, adhesion and specific epithelial differentiation.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215112001661_sec3\"><jats:title>Results:<\/jats:title><jats:p>The silk fibroin scaffolds successfully supported the growth and adhesion of keratinocytes, whilst also maintaining their cell lineage.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215112001661_sec4\"><jats:title>Conclusion:<\/jats:title><jats:p>The properties of silk fibroin make it an attractive option for further research, as a potential alternative graft in myringoplasty.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s0022215112001661","type":"journal-article","created":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T06:29:43Z","timestamp":1345012183000},"page":"S13-S20","source":"Crossref","is-referenced-by-count":38,"title":["Utilising silk fibroin membranes as scaffolds for the growth of tympanic 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