{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:15:40Z","timestamp":1778652940695,"version":"3.51.4"},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T00:00:00Z","timestamp":1424304000000},"content-version":"unspecified","delay-in-days":18,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Laryngol. Otol."],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0022215114003351_sec1\"><jats:title>Background:<\/jats:title><jats:p>Genetic alteration of cyclin-dependent kinase inhibitors has been associated with carcinogenesis mechanisms in various organs.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215114003351_sec2\"><jats:title>Objective:<\/jats:title><jats:p>This study aimed to evaluate the expression and mutational analysis of Cip\/Kip family cyclin-dependent kinase inhibitors (p21<jats:sup>CIP1\/WAF1<\/jats:sup>, p27<jats:sup>KIP1<\/jats:sup> and p57<jats:sup>KIP2<\/jats:sup>) in early glottic cancer.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215114003351_sec3\"><jats:title>Methods:<\/jats:title><jats:p>Expressions of Cip\/Kip family and p53 were determined by quantitative reverse transcription polymerase chain reaction and densitometry. For the analysis of p21 inactivation, sequence alteration was assessed using single-strand conformational polymorphism polymerase chain reaction. Additionally, the inactivation mechanism of p27 and p57 were investigated using DNA methylation analysis.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215114003351_sec4\"><jats:title>Results:<\/jats:title><jats:p>Reduced expression of p27 and p57 were detected in all samples, whereas the expression of p21 was incompletely down-regulated in 6 of 11 samples. Additionally, single-strand conformational polymorphism polymerase chain reaction analysis showed the p53 mutation at exon 6. Methylation of p27 and p57 was detected by DNA methylation assay.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215114003351_sec5\"><jats:title>Conclusion:<\/jats:title><jats:p>Our results suggest that the Cip\/Kip family may have a role as a molecular mechanism of carcinogenesis in early glottic cancer.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s0022215114003351","type":"journal-article","created":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T14:59:27Z","timestamp":1424357967000},"page":"168-173","source":"Crossref","is-referenced-by-count":4,"title":["Expression and mutational analysis of Cip\/Kip family in early glottic cancer"],"prefix":"10.1017","volume":"129","author":[{"given":"D-K","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J H","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O J","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C H","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2015,2,19]]},"reference":[{"key":"S0022215114003351_ref9","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2000.14"},{"key":"S0022215114003351_ref11","doi-asserted-by":"crossref","first-page":"661","DOI":"10.4103\/0377-4929.72019","article-title":"Expression of Ki67 and P53 in primary squamous cell carcinoma of the larynx","volume":"53","author":"Ashraf","year":"2010","journal-title":"Indian J Pathol Microbiol"},{"key":"S0022215114003351_ref6","first-page":"1581","article-title":"Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins","volume":"11","author":"Hall","year":"1995","journal-title":"Oncogene"},{"key":"S0022215114003351_ref12","first-page":"542","article-title":"Immunohistochemical localization of glutathione s-transferase isoenzymes (gsta, Gstp, Gstm4, and Gstt1) and tumour marker p53 in matched tissue from normal larynx and laryngeal carcinoma: correlations with prognostic factors","volume":"39","author":"Sedat","year":"2010","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"S0022215114003351_ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-012-1937-3"},{"key":"S0022215114003351_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0964-1955(96)00002-4"},{"key":"S0022215114003351_ref17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6791"},{"key":"S0022215114003351_ref14","first-page":"719","article-title":"Expression of cyclin-dependent kinases inhibitors p21 (WAF1) and p27 (KIP1) in benign, premalignant and malignant laryngeal lesions. Correlation with cell cycle regulatory proteins","volume":"18","author":"Peschos","year":"2004","journal-title":"In Vivo"},{"key":"S0022215114003351_ref3","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200301000-00031"},{"key":"S0022215114003351_ref2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199803000-00007"},{"key":"S0022215114003351_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(99)80026-4"},{"key":"S0022215114003351_ref15","first-page":"262","article-title":"Mechanism for inactivation of the KIP family cyclin-dependent kinase inhibitor genes in gastric cancer cells","volume":"60","author":"Shin","year":"2000","journal-title":"Cancer Res"},{"key":"S0022215114003351_ref19","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.6.639"},{"key":"S0022215114003351_ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200203000-00013"},{"key":"S0022215114003351_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90572-X"},{"key":"S0022215114003351_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90237-8"},{"key":"S0022215114003351_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(96)10030-1"},{"key":"S0022215114003351_ref8","first-page":"1947","article-title":"Immunohistochemical characterization of p57KIP2 expression in human esophageal squamous cell carcinoma","volume":"20","author":"Matsumoto","year":"2000","journal-title":"Anticancer Res"},{"key":"S0022215114003351_ref10","first-page":"1159","article-title":"High frequency of p53 gene alterations associated with protein overexpression in human squamous cell carcinoma of the larynx","volume":"7","author":"Maestro","year":"1992","journal-title":"Oncogene"}],"container-title":["The Journal of Laryngology &amp; Otology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022215114003351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T10:43:46Z","timestamp":1643366626000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022215114003351\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S0022215114003351"],"URL":"https:\/\/doi.org\/10.1017\/s0022215114003351","relation":{},"ISSN":["0022-2151","1748-5460"],"issn-type":[{"value":"0022-2151","type":"print"},{"value":"1748-5460","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2]]}}}