{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:44:32Z","timestamp":1757591072589},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Laryngol. Otol."],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0022215121002723_sec_a1\"><jats:title>Objective<\/jats:title><jats:p>To determine the prevalence of human papillomavirus in paediatric tonsils in Southwestern Ontario, Canada.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215121002723_sec_a2\" sec-type=\"materials\"><jats:title>Materials and methods<\/jats:title><jats:p>Patients aged 0\u201318 years undergoing tonsillectomy were recruited. Two specimens (left and right tonsils) were collected from each participant. Tonsillar DNA was analysed using quantitative polymerase chain reaction to determine the presence of human papillomavirus subtypes 6, 11, 16 or 18.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215121002723_sec_a3\" sec-type=\"results\"><jats:title>Results<\/jats:title><jats:p>A total of 102 patients, aged 1\u201318 years (mean age of 5.7 years), were recruited. Ninety-nine surveys were returned. There were 44 females (44.4 per cent) and 55 males (55.6 per cent). Forty patients (40.4 per cent) were firstborn children and 73 (73.7 per cent) were delivered vaginally. Six mothers (6.1 per cent) and one father (1.0 per cent) had prior known human papillomavirus infection, and one mother (1.0 per cent) had a history of cervical cancer. All tonsil specimens were negative for human papillomavirus subtypes 6, 11, 16 and 18.<\/jats:p><\/jats:sec><jats:sec id=\"S0022215121002723_sec_a4\" sec-type=\"conclusions\"><jats:title>Conclusion<\/jats:title><jats:p>No human papillomavirus subtypes 6, 11, 16 or 18 were found in paediatric tonsil specimens from Southwestern Ontario.<\/jats:p><\/jats:sec>","DOI":"10.1017\/s0022215121002723","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T08:36:41Z","timestamp":1633336601000},"page":"243-247","source":"Crossref","is-referenced-by-count":2,"title":["The prevalence of human papillomavirus in paediatric tonsils in Southwestern Ontario"],"prefix":"10.1017","volume":"136","author":[{"given":"M","family":"Wojtera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J W","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J E","family":"Strychowsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Dzioba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Paradis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Husein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M I","family":"Salvadori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A C","family":"Nichols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"key":"S0022215121002723_ref15","doi-asserted-by":"publisher","DOI":"10.4103\/ijpvm.IJPVM_49_17"},{"key":"S0022215121002723_ref2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6606091"},{"key":"S0022215121002723_ref10","unstructured":"10 Qiagen. AllPrep DNA\/RNA Mini Handbook: For simultaneous purification of genomic DNA and total RNA from the same animal cell or tissue sample. In: https:\/\/www.qiagen.com\/us\/resources\/download.aspx?id=580866a6-56c6-4674-8566-2852164d8519&lang=en [29 September 2021]"},{"key":"S0022215121002723_ref16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000270"},{"key":"S0022215121002723_ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-012-0372-5"},{"key":"S0022215121002723_ref5","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.008656"},{"key":"S0022215121002723_ref7","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20011201)92:11<2875::AID-CNCR10130>3.0.CO;2-7"},{"key":"S0022215121002723_ref13","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25396"},{"key":"S0022215121002723_ref4","doi-asserted-by":"publisher","DOI":"10.1002\/hed.22015"},{"key":"S0022215121002723_ref11","unstructured":"11 Merck Canada. \u201cJuvenile-Onset Recurrent Respiratory Papillomatosis in the Era of HPV\u201d and \u201cVaccination Immunotherapy-Updates in the Treatment of Head and Neck Cancer\u201d. Paper presented at: Canadian Society of Otolaryngology \u2013 Head and Neck Surgery 72nd Annual General Meeting, June 17\u201319, 2018, Quebec City, QC. In: https:\/\/www.entcanada.org\/news-events\/annual-meetings\/quebec-city-2018\/ [29 September 2021]"},{"key":"S0022215121002723_ref6","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djg107"},{"key":"S0022215121002723_ref1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.101"},{"key":"S0022215121002723_ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1916-0216-42-9"},{"key":"S0022215121002723_ref3","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.14.1713"},{"key":"S0022215121002723_ref8","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-018-0255-1"},{"key":"S0022215121002723_ref12","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-04-0812"}],"container-title":["The Journal of Laryngology &amp; Otology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022215121002723","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T14:15:16Z","timestamp":1647267316000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022215121002723\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,4]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["S0022215121002723"],"URL":"https:\/\/doi.org\/10.1017\/s0022215121002723","relation":{},"ISSN":["0022-2151","1748-5460"],"issn-type":[{"value":"0022-2151","type":"print"},{"value":"1748-5460","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,4]]}}}